GLI1 (NM_001167609) Human Untagged Clone

CAT#: SC329277

GLI1 (untagged)-Human GLI family zinc finger 1 (GLI1) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLI1
• Synonyms: GLI; PAPA8; PPD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC329277 representing NM_001167609.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTCAACTCGATGACCCCACCACCAATCAGTAGCTATGGCGAGCCCTGCTGTCTCCGGCCCCTCCCC
  AGTCAGGGGGCCCCCAGTGTGGGGACAGAAGTCAAGTTGACCAAGAAGCGGGCACTGTCCATCTCACCT
  CTGTCGGATGCCAGCCTGGACCTGCAGACGGTTATCCGCACCTCACCCAGCTCCCTCGTAGCTTTCATC
  AACTCGCGATGCACATCTCCAGGAGGCTCCTACGGTCATCTCTCCATTGGCACCATGAGCCCATCTCTG
  GGATTCCCAGCCCAGATGAATCACCAAAAAGGGCCCTCGCCTTCCTTTGGGGTCCAGCCTTGTGGTCCC
  CATGACTCTGCCCGGGGTGGGATGATCCCACATCCTCAGTCCCGGGGACCCTTCCCAACTTGCCAGCTG
  AAGTCTGAGCTGGACATGCTGGTTGGCAAGTGCCGGGAGGAACCCTTGGAAGGTGATATGTCCAGCCCC
  AACTCCACAGGCATACAGGATCCCCTGTTGGGGATGCTGGATGGGCGGGAGGACCTCGAGAGAGAGGAG
  AAGCGTGAGCCTGAATCTGTGTATGAAACTGACTGCCGTTGGGATGGCTGCAGCCAGGAATTTGACTCC
  CAAGAGCAGCTGGTGCACCACATCAACAGCGAGCACATCCACGGGGAGCGGAAGGAGTTCGTGTGCCAC
  TGGGGGGGCTGCTCCAGGGAGCTGAGGCCCTTCAAAGCCCAGTACATGCTGGTGGTTCACATGCGCAGA
  CACACTGGCGAGAAGCCACACAAGTGCACGTTTGAAGGGTGCCGGAAGTCATACTCACGCCTCGAAAAC
  CTGAAGACGCACCTGCGGTCACACACGGGTGAGAAGCCATACATGTGTGAGCACGAGGGCTGCAGTAAA
  GCCTTCAGCAATGCCAGTGACCGAGCCAAGCACCAGAATCGGACCCATTCCAATGAGAAGCCGTATGTA
  TGTAAGCTCCCTGGCTGCACCAAACGCTATACAGATCCTAGCTCGCTGCGAAAACATGTCAAGACAGTG
  CATGGTCCTGACGCCCATGTGACCAAACGGCACCGTGGGGATGGCCCCCTGCCTCGGGCACCATCCATT
  TCTACAGTGGAGCCCAAGAGGGAGCGGGAAGGAGGTCCCATCAGGGAGGAAAGCAGACTGACTGTGCCA
  GAGGGTGCCATGAAGCCACAGCCAAGCCCTGGGGCCCAGTCATCCTGCAGCAGTGACCACTCCCCGGCA
  GGGAGTGCAGCCAATACAGACAGTGGTGTGGAAATGACTGGCAATGCAGGGGGCAGCACTGAAGACCTC
  TCCAGCTTGGACGAGGGACCTTGCATTGCTGGCACTGGTCTGTCCACTCTTCGCCGCCTTGAGAACCTC
  AGGCTGGACCAGCTACATCAACTCCGGCCAATAGGGACCCGGGGTCTCAAACTGCCCAGCTTGTCCCAC
  ACCGGTACCACTGTGTCCCGCCGCGTGGGCCCCCCAGTCTCTCTTGAACGCCGCAGCAGCAGCTCCAGC
  AGCATCAGCTCTGCCTATACTGTCAGCCGCCGCTCCTCCCTGGCCTCTCCTTTCCCCCCTGGCTCCCCA
  CCAGAGAATGGAGCATCCTCCCTGCCTGGCCTTATGCCTGCCCAGCACTACCTGCTTCGGGCAAGATAT
  GCTTCAGCCAGAGGGGGTGGTACTTCGCCCACTGCAGCATCCAGCCTGGATCGGATAGGTGGTCTTCCC
  ATGCCTCCTTGGAGAAGCCGAGCCGAGTATCCAGGATACAACCCCAATGCAGGGGTCACCCGGAGGGCC
  AGTGACCCAGCCCAGGCTGCTGACCGTCCTGCTCCAGCTAGAGTCCAGAGGTTCAAGAGCCTGGGCTGT
  GTCCATACCCCACCCACTGTGGCAGGGGGAGGACAGAACTTTGATCCTTACCTCCCAACCTCTGTCTAC
  TCACCACAGCCCCCCAGCATCACTGAGAATGCTGCCATGGATGCTAGAGGGCTACAGGAAGAGCCAGAA
  GTTGGGACCTCCATGGTGGGCAGTGGTCTGAACCCCTATATGGACTTCCCACCTACTGATACTCTGGGA
  TATGGGGGACCTGAAGGGGCAGCAGCTGAGCCTTATGGAGCGAGGGGTCCAGGCTCTCTGCCTCTTGGG
  CCTGGTCCACCCACCAACTATGGCCCCAACCCCTGTCCCCAGCAGGCCTCATATCCTGACCCCACCCAA
  GAAACATGGGGTGAGTTCCCTTCCCACTCTGGGCTGTACCCAGGCCCCAAGGCTCTAGGTGGAACCTAC
  AGCCAGTGTCCTCGACTTGAACATTATGGACAAGTGCAAGTCAAGCCAGAACAGGGGTGCCCAGTGGGG
  TCTGACTCCACAGGACTGGCACCCTGCCTCAATGCCCACCCCAGTGAGGGGCCCCCACATCCACAGCCT
  CTCTTTTCCCATTACCCCCAGCCCTCTCCTCCCCAATATCTCCAGTCAGGCCCCTATACCCAGCCACCC
  CCTGATTATCTTCCTTCAGAACCCAGGCCTTGCCTGGACTTTGATTCCCCCACCCATTCCACAGGGCAG
  CTCAAGGCTCAGCTTGTGTGTAATTATGTTCAATCTCAACAGGAGCTACTGTGGGAGGGTGGGGGCAGG
  GAAGATGCCCCCGCCCAGGAACCTTCCTACCAGAGTCCCAAGTTTCTGGGGGGTTCCCAGGTTAGCCCA
  AGCCGTGCTAAAGCTCCAGTGAACACATATGGACCTGGCTTTGGACCCAACTTGCCCAATCACAAGTCA
  GGTTCCTATCCCACCCCTTCACCATGCCATGAAAATTTTGTAGTGGGGGCAAATAGGGCTTCACATAGG
  GCAGCAGCACCACCTCGACTTCTGCCCCCATTGCCCACTTGCTATGGGCCTCTCAAAGTGGGAGGCACA
  AACCCCAGCTGTGGTCATCCTGAGGTGGGCAGGCTAGGAGGGGGTCCTGCCTTGTACCCTCCTCCCGAA
  GGACAGGTATGTAACCCCCTGGACTCTCTTGATCTTGACAACACTCAGCTGGACTTTGTGGCTATTCTG
  GATGAGCCCCAGGGGCTGAGTCCTCCTCCTTCCCATGATCAGCGGGGCAGCTCTGGACATACCCCACCT
  CCCTCTGGGCCCCCCAACATGGCTGTGGGCAACATGAGTGTCTTACTGAGATCCCTACCTGGGGAAACA
  GAATTCCTCAACTCTAGTGCCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001167609
• Insert Size: 3198 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167609.1
• RefSeq Size: 3483 bp
• RefSeq ORF: 3198 bp
• Locus ID: 2735
• UniProt ID: P08151
• Cytogenetics: 12q13.3
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
• MW: 113.7 kDa
• Gene Summary: This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
  Transcript Variant: This variant (3) lacks an in-frame segment in the 5' coding region, compared to variant 1, resulting in a shorter protein isoform (3), also known as tGLI1. This variant reflects the transcript and protein described by Lo (PubMed ID 19706761). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.