PCDH20 (NM_022843) Human Untagged Clone

CAT#: SC328032

PCDH20 (untagged)-Human protocadherin 20 (PCDH20)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCDH20
• Synonyms: PCDH13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC328032 representing NM_022843.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCGCGGCCGAGGGAATGCGCGCAGCTCACAGGCCCTGGGAGTGAGCTGGTGCCCGGCGACCTGGCAC
  CCGCGCCTGGATATGGGGCGTCTACATCGTCCCAGGAGCAGCACCAGCTACAGGAACCTGCCGCATCTG
  TTTCTGTTTTTCCTCTTCGTGGGACCCTTCAGCTGCCTCGGGAGTTACAGCCGGGCCACCGAGCTTCTG
  TACAGCCTAAACGAGGGACTACCCGCGGGGGTGCTCATCGGCAGCCTGGCCGAGGACCTGCGGCTGCTG
  CCCAGGTCTGCAGGGAGGCCGGACCCGCAGTCGCAGCTGCCAGAGCGCACCGGTGCTGAGTGGAACCCC
  CCTCTCTCCTTCAGCCTGGCCTCCCGGGGACTGAGTGGCCAGTACGTGACCCTAGACAACCGCTCTGGG
  GAGCTGCACACTTCAGCTCAGGAGATCGACAGGGAGGCCCTGTGTGTTGAAGGGGGTGGAGGGACTGCG
  TGGAGCGGCAGCGTTTCCATCTCCTCCTCTCCTTCTGACTCTTGTCTTTTGCTGCTGGATGTGCTTGTC
  CTGCCTCAGGAATACTTCAGGTTTGTGAAGGTGAAGATCGCCATCAGAGACATCAATGACAACGCCCCG
  CAGTTCCCTGTTTCCCAGATCTCGGTGTGGGTCCCGGAAAATGCACCTGTAAACACCCGACTGGCCATA
  GAGCATCCTGCTGTGGACCCAGATGTAGGCATTAATGGGGTACAGACCTATCGCTTACTGGACTACCAT
  GGTATGTTCACCCTGGACGTGGAGGAGAATGAGAATGGGGAGCGCACCCCCTACCTAATTGTCATGGGT
  GCTTTGGACAGGGAAACCCAGGACCAGTATGTGAGCATCATCATAGCTGAGGATGGTGGGTCTCCACCA
  CTTTTGGGCAGTGCCACTCTCACCATTGGCATCAGTGACATTAATGACAATTGCCCTCTCTTCACAGAC
  TCACAAATCAATGTCACTGTGTATGGGAATGCTACAGTGGGCACCCCAATTGCAGCTGTCCAGGCTGTG
  GATAAAGACTTGGGGACCAATGCTCAAATTACTTATTCTTACAGTCAGAAAGTTCCACAAGCATCTAAG
  GATTTATTTCACCTGGATGAAAACACTGGAGTCATTAAACTTTTCAGTAAGATTGGAGGAAGTGTTCTG
  GAGTCCCACAAGCTCACCATCCTTGCTAATGGACCAGGCTGCATCCCTGCTGTAATCACTGCTCTTGTG
  TCCATTATTAAAGTTATTTTCAGACCCCCTGAAATTGTCCCTCGTTACATAGCAAACGAGATAGATGGT
  GTGGTTTATCTGAAAGAACTGGAACCCGTTAACACTCCCATTGCGTTTTTCACCATAAGAGATCCAGAA
  GGTAAATACAAGGTTAACTGCTACCTGGATGGTGAAGGGCCGTTTAGGTTATCACCTTACAAACCATAC
  AATAATGAATATTTACTAGAGACCACAAAACCTATGGACTATGAGCTACAGCAGTTCTATGAAGTAGCT
  GTGGTGGCTTGGAACTCTGAGGGATTTCATGTCAAAAGGGTCATTAAAGTGCAACTTTTAGATGACAAT
  GATAATGCTCCAATTTTCCTTCAACCCTTAATAGAACTAACCATCGAAGAGAACAACTCACCCAATGCC
  TTTTTGACTAAGCTGTATGCTACAGATGCCGACAGCGAGGAGAGAGGCCAAGTTTCATATTTTCTGGGA
  CCTGATGCTCCATCATATTTTTCCTTAGACAGTGTCACAGGAATTCTGACAGTTTCTACTCAGCTGGAC
  CGAGAAGAGAAAGAAAAGTACAGATACACTGTCAGAGCTGTTGACTGTGGGAAGCCACCCAGAGAATCA
  GTAGCCACTGTGGCCCTCACAGTGTTGGATAAAAATGACAACAGTCCTCGGTTTATCAACAAGGACTTC
  AGCTTTTTTGTGCCTGAAAACTTTCCAGGCTATGGTGAGATTGGAGTAATTAGTGTAACAGATGCTGAC
  GCTGGACGAAATGGATGGGTCGCCCTCTCTGTGGTGAACCAGAGTGATATTTTTGTCATAGATACAGGA
  AAGGGTATGCTGAGGGCTAAAGTCTCTTTGGACAGAGAGCAGCAAAGCTCCTATACTTTGTGGGTTGAA
  GCTGTTGATGGGGGTGAGCCTGCCCTCTCCTCTACAGCAAAAATCACAATTCTCCTTCTAGATATCAAT
  GACAACCCTCCTCTTGTTTTGTTTCCTCAGTCTAATATGTCTTATCTGTTAGTACTGCCTTCTACTCTG
  CCAGGCTCCCCGGTTACAGAAGTCTATGCTGTCGACAAAGACACAGGCATGAATGCTGTCATAGCTTAC
  AGCATCATAGGGAGAAGAGGTCCTAGGCCTGAGTCCTTCAGGATTGACCCTAAAACTGGCAACATTACT
  TTGGAAGAGGCATTGCTGCAGACAGATTATGGGCTCCATCGCTTACTGGTGAAAGTGAGTGATCATGGT
  TATCCCGAGCCTCTCCACTCCACAGTCATGGTGAACCTATTTGTCAATGACACTGTCAGTAATGAGAGT
  TACATTGAGAGTCTTTTAAGAAAAGAACCAGAGATTAATATAGAGGAGAAAGAACCACAAATCTCAATA
  GAACCGACTCATAGGAAGGTAGAATCTGTGTCTTGTATGCCCACCTTAGTAGCTCTGTCTGTAATAAGC
  TTGGGTTCCATCACACTGGTCACAGGGATGGGCATATACATCTGTTTAAGGAAAGGGGAAAAGCATCCC
  AGGGAAGATGAAAATTTGGAAGTACAGATTCCACTGAAAGGAAAAATTGACTTGCATATGCGAGAGAGA
  AAGCCAATGGATATTTCTAATATTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_022843
• Insert Size: 2856 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022843.3
• RefSeq Size: 4786 bp
• RefSeq ORF: 2856 bp
• Locus ID: 64881
• UniProt ID: Q8N6Y1
• Cytogenetics: 13q21.2
• Domains: CA
• Protein Families: Druggable Genome, Transmembrane
• MW: 104.9 kDa
• Gene Summary: This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. This gene encodes a protein which contains 6 extracellular cadherin domains, a transmembrane domain and a cytoplasmic tail differing from those of the classical cadherins. Although its specific function is undetermined, the cadherin-related neuronal receptor is thought to play a role in the establishment and function of specific cell-cell connections in the brain. [provided by RefSeq, Jul 2008]