FMO5 (NM_001144829) Human Untagged Clone

CAT#: SC325963

FMO5 (untagged)-Human flavin containing monooxygenase 5 (FMO5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FMO5
• Synonyms: hBVMO1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC325963 representing NM_001144829.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACTAAGAAAAGAATTGCTGTGATTGGGGGAGGAGTGAGCGGGCTCTCTTCCATCAAGTGCTGCGTA
  GAAGAAGGCTTGGAACCTGTCTGCTTTGAAAGGACTGATGACATCGGAGGGCTCTGGAGGTTCCAGGAA
  AATCCTGAAGAAGGAAGGGCCAGTATTTACAAATCAGTGATCATCAATACTTCTAAAGAGATGATGTGC
  TTCAGTGACTATCCAATCCCAGATCATTATCCCAACTTCATGCATAATGCCCAGGTCCTGGAGTATTTC
  AGGATGTATGCCAAAGAATTTGACCTTCTAAAGTATATTCGATTTAAGACCACTGTGTGCAGTGTGAAG
  AAGCAGCCTGATTTTGCCACTTCAGGCCAATGGGAAGTGGTCACTGAATCTGAAGGGAAAAAGGAGATG
  AATGTCTTTGATGGAGTCATGGTTTGCACTGGCCATCACACCAATGCTCATCTACCTCTGGAAAGCTTC
  CCTGGAATTGAGAAGTTCAAAGGGCAGTACTTCCACAGTCGAGACTATAAGAACCCAGAGGGATTCACT
  GGAAAGAGAGTCATTATAATTGGCATTGGGAATTCTGGAGGGGATCTGGCTGTAGAGATTAGCCAAACA
  GCCAAGCAGGTTTTCCTCAGCACCAGGAGAGGGGCTTGGATCCTGAATCGTGTAGGGGACTACGGATAT
  CCTGCTGATGTGTTGTTCTCTTCTCGACTTACACATTTTATATGGAAGATCTGTGGCCAATCATTAGCA
  AACAAATATTTGGAAAAAAAGATAAACCAAAGGTTTGACCATGAAATGTTTGGCCTGAAGCCTAAACAC
  AGAGCTCTGAGTCAGCATCCAACCTTAAATGATGACCTGCCAAATCGTATCATTTCTGGCTTGGTGAAA
  GTGAAAGGAAATGTGAAGGAATTCACGGAGACAGCTGCCATATTTGAGGATGGCTCCAGGGAGGATGAC
  ATTGATGCTGTTATCTTTGCCACAGGCTATAGCTTTGACTTTCCATTTCTGGAAGATTCCGTCAAAGTG
  GTCAAAAACAAGATATCCCTGTATAAAAAGGTCTTCCCTCCTAACCTGGAAAGGCCAACTCTTGCAATC
  ATAGGCTTGATTCAGCCCTTAGGAGCCATTATGCCCATTTCAGAGCTCCAAGGACGCTGGGCCACTCAG
  GTATTTAAAGGTCTAAAGACATTGCCCTCACAGAGTGAAATGATGGCAGAAATATCTAAAGCTCAAGAG
  GAAATTGACAAAAGTCTAACCATGAGAAAAACATCAGACAAACCCAAGTTGAAGAATATTCTACAAATA
  CCTGACTACCTCAAAACTGTCAAGATCATCAACAAAGAAAGTCTGAGAAATTGTCCCGGAATAAAGGGG
  CCTAAGGAGACATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001144829
• Insert Size: 1395 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001144829.2
• RefSeq Size: 2014 bp
• RefSeq ORF: 1395 bp
• Locus ID: 2330
• UniProt ID: P49326
• Cytogenetics: 1q21.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450
• MW: 52.5 kDa
• Gene Summary: Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR and contains an alternate 3' terminal exon including coding region, compared to variant 1. The resulting isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.