IMPA1 (NM_001144878) Human Untagged Clone

CAT#: SC325787

IMPA1 (untagged)-Human inositol(myo)-1(or 4)-monophosphatase 1 (IMPA1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IMPA1
• Synonyms: IMP; IMPA; MRT59
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC325787 representing NM_001144878.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGGCAGCGCCCAGGTCCTGTGCTGCCCGCAGTCGCGGTGCTGGGACAGGTGGCAAAAAGGAAGGTG
  GCCTGGCTCCTACGCTGGAAGGCTGTGACCAGGACAGAAACAGCAGGAAACAGTTCAGGTGTTTATGGC
  TTTGGGAAAATGAAAATATTTGTCAAATATTTTCAGAAGATGGCTGATCCTTGGCAGGAATGCATGGAT
  TATGCAGTAACTCTAGCAAGACAAGCTGGAGAGGTAGTTTGTGAAGCTATAAAAAATGAAATGAATGTT
  ATGCTGAAAAGTTCTCCAGTTGATTTGGTAACTGCTACGGACCAAAAAGTTGAAAAAATGCTTATCTCT
  TCCATAAAGGAAAAGTATCCATCTCACAGTTTCATTGGTGAAGAATCTGTGGCAGCTGGGGAAAAAAGT
  ATCTTAACCGACAACCCCACATGGATCATTGACCCTATTGATGGAACAACTAACTTTGTACATAGATTT
  CCTTTTGTAGCTGTTTCAATTGGCTTTGCTGTAAATAAAAAGATAGAATTTGGAGTTGTGTACAGTTGT
  GTGGAAGGCAAGATGTACACTGCCAGAAAAGGAAAAGGTGCCTTTTGTAATGGTCAAAAACTACAAGTT
  TCACAACAAGAAGATATTACCAAATCTCTCTTGGTGACTGAGTTGGGCTCTTCCAGAACACCAGAGACT
  GTGAGAATGGTTCTTTCTAATATGGAAAAGCTTTTTTGCATTCCTGTTCATGGGATCCGGAGTGTTGGA
  ACAGCAGCTGTTAATATGTGCCTTGTGGCAACTGGCGGAGCAGATGCATATTATGAAATGGGAATTCAC
  TGCTGGGATGTTGCAGGAGCTGGCATTATTGTTACTGAAGCTGGTGGCGTGCTAATGGATGTTACAGGT
  GGACCATTTGATTTGATGTCACGAAGAGTAATTGCTGCAAATAATAGAATATTAGCAGAAAGGATAGCT
  AAAGAAATTCAGGTTATACCTTTGCAACGAGACGACGAAGATTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001144878
• Insert Size: 1011 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001144878.1
• RefSeq Size: 3597 bp
• RefSeq ORF: 1011 bp
• Locus ID: 3612
• UniProt ID: P29218
• Cytogenetics: 8q21.13
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 36.7 kDa
• Gene Summary: This gene encodes an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13. [provided by RefSeq, Dec 2014]
  Transcript Variant: This variant (2) contains an additional in-frame coding exon compared to transcript variant 1, resulting in an isoform (2) with a longer and a novel N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no quality transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.