NDRG1 (NM_001135242) Human Untagged Clone

CAT#: SC325042

NDRG1 (untagged)-Human N-myc downstream regulated 1 (NDRG1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDRG1
• Synonyms: CAP43; CMT4D; DRG-1; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001135242 edited
  CTGGTGCTGAAGCTCGTCAGTTCACCATCCGCCCTCGGCTTCCGCGGGGCGCTGGGCCGC
  CAGCCTCGGCACCGTCCTTTCCTTTCTCCCTCGCGTTAGGCAGGTGACAGCAGGGACATG
  TCTCGGGAGATGCAGGATGTAGACCTCGCTGAGGTGAAGCCTTTGGTGGAGAAAGGGGAG
  ACCATCACCGGCCTCCTGCAAGAGTTTGATGTCCAGGAGCAGGACATCGAGACTTTACAT
  GGCTCTGTTCACGTCACGCTGTGTGGGACTCCCAAG:GGAAACCGGCCTGTCATCCTCAC
  CTACCATGACATCGGCATGAACCACAAAACCTGCTACAACCCCCTCTTCAACTACGAGGA
  CATGCAGGAGATCACCCAGCACTTTGCCGTCTGCCACGTGGACGCCCCTGGCCAGCAGGA
  CGGCGCAGCCTCCTTCCCCGCAGGGTACATGTACCCCTCCATGGATCAGCTGGCTGAAAT
  GCTTCCTGGAGTCCTTCAACAGTTTGGGCTGAAAAGCATTATTGGCATGGGAACAGGAGC
  AGGCGCCTACATCCTAACTCGATTTGCTCTAAACAACCCTGAGATGGTGGAGGGCCTTGT
  CCTTATCAACGTGAACCCTTGTGCGGAAGGCTGGATGGACTGGGCCGCCTCCAAGATCTC
  AGGATGGACCCAAGCTCTGCCGGACATGGTGGTGTCCCACCTTTTTGGGAAGGAAGAAAT
  GCAGAGTAACGTGGAAGTGGTCCACACCTACCGCCAGCACATTGTGAATGACATGAACCC
  CGGCAACCTGCACCTGTTCATCAATGCCTACAACAGCCGGCGCGACCTGGAGATTGAGCG
  ACCAATGCCGGGAACCCACACAGTCACCCTGCAGTGCCCTGCTCTGTTGGTGGTTGGGGA
  CAGCTCGCCTGCAGTGGATGCCGTGGTGGAGTGCAACTCAAAATTGGACCCAACAAAGAC
  CACTCTCCTCAAGATGGCGGACTGTGGCGGCCTCCCGCAGATCTCCCAGCCGGCCAAGCT
  CGCTGAGGCCTTCAAGTACTTCGTGCAGGGCATGGGATACATGCCCTCGGCTAGCATGAC
  CCGCCTGATGCGGTCCCGCACAGCCTCTGGTTCCAGCGTCACTTCTCTGGATGGCACCCG
  CAGCCGCTCCCACACCAGCGAGGGCACCCGAAGCCGCTCCCACACCAGCGAGGGCACCCG
  CAGCCGCTCGCACACCAGCGAGGGGGCCCACCTGGACATCACCCCCAACTCGGGTGCTGC
  TGGGAACAGCGCCGGGCCCAAGTCCATGGAGGTCTCCTGCTAGGCGGCCTGCCCAGCTGC
  CGCCCCCGGACTCTGATCTCTGTAGTGGCCCCCTCCTCCCCGGCCCCTTTTCGCCCCCTG
  CCTGCCATACTGCGCCTAACTCGGTATTAATCCAAAGCTTATTTTGTAAGAGTGAGCTCT
  GGTGGAGACAAATGAGGTCTATTACGTGGGTGCCCTCTCCAAAGGCGGGGTGGCGGTGGA
  CCAAAGGAAGGAAGCAAGCATCTCCGCATCGCATCCTCTTCCATTAACCAGTGGCCGGTT
  GCCACTCTCCTCCCCTCCCTCAGAGACACCAAACTGCCAAAAACAAGACGCGTAGCAGCA
  CACACTTCACAAAGCCAAGCCTAGGCCGCCCTGAGCATCCTGGTTCAAACGGGTGCCTGG
  TCAGAAGGCCAGCCGCCCACTTCCCGTTTCCTCTTTAACTGAGGAGAAGCTGATCCAGTT
  TCCGGAAACAAAATCCTTTTCTCATTTGGGGAGGGGGGTAATAGTGACATGCAGGCACCT
  CTTTTAAACAGGCAAAACAGGAAGGGGGAAAAGGTGGGATTCATGTCGAGGCTAGAGGCA
  TTTGGAACAACAAATCTACGTAGTTAACTTGAAGAAACCGATTTTTAAAGTTGGTGCATC
  TAGAAAGCTTTGAATGCAGAAGCAAACAAGCTTGATTTTTCTAGCATCCTCTTAATGTGC
  AGCAAAAGCAGGCAACAAAATCTCCTGGCTTTACAGACAAAAATATTTCAGCAAACGTTG
  GGCATCATGGTTTTTGAAGGCTTTAGTTCTGCTTTCTGCCTCTCCTCCACAGCCCCAACC
  TCCCACCCCTGATACATGAGCCAGTGATTATTCTTGTTCAGGGAGAAGATCATTTAGATT
  TGTTTTGCATTCCTTAGAATGGAGGGCAACATTCCACAGCTGCCCTGGCTGTGATGAGTG
  TCCTTGCAGGGGCCGGAGTAGGAGCACTGGGGTGGGGGCGGAATTGGGGTTACTCGATGT
  AAGGGATTCCTTGTTGTTGTGTTGAGATCCAGTGCAGTTGTGATTTCTGTGGATCCCAGC
  TTGGTTCCAGGAATTTTGTGTGATTGGCTTAAATCCAGTTTTCAATCTTCGACAGCTGGG
  CTGGAACGTGAACTCAGTAGCTGAACCTGTCTGACCCGGTCACGTTCTTGGATCCTCAGA
  ACTCTTGCTCTTGTCGGGGTGGGGGTGGGAACTCACGTGGGGAGCGGTGGCTGAGAAAAT
  GTAAGGATTCTGGAATACATATTCCATGGGACTTTCCTTCCCTCTCCTGCTTCCTCTTTT
  CCTGCTCCCTAACCTTTCGCCGAATGGGGCAGCACCACTGACGTTTCTGGGCGGCCAGTG
  CGGCTGCCAGGTTCCTGTACTACTGCCTTGTACTTTTCATTTTGGCTCACCGTGGATTTT
  CTCATAGGAAGTTTGGTCAGAGTGAATTGAATATTGTAAGTCAGCCACTGGGACCCGAGG
  ATTTCTGGGACCCCGCAGTTGGGAGGAGGAAGTAGTCCAGCCTTCCAGGTGGCGTGAGAG
  GCAATGACTCGTTACCTGCCGCCCATCACCTTGGAGGCCTTCCCTGGCCTTGAGTAGAAA
  AGTCGGGGATCGGGGCAAGAGAGGCTGAGTACGGATGGGAAACTATTGTGCACAAGTCTT
  TCCAGAGGAGTTTCTTAATGAGATATTTGTATTTATTTCCAGACCAATAAATTTGTAACT
  TTGCAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001135242
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001135242.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135242.1, NP_001128714.1
• RefSeq Size: 3520 bp
• RefSeq ORF: 1185 bp
• Locus ID: 10397
• UniProt ID: Q92597
• Cytogenetics: 8q24.22
• Gene Summary: This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same isoform (1).