NDRG1 (NM_001135242) Human Tagged ORF Clone

CAT#: RC225609

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  • TrueORF®

NDRG1 (Myc-DDK-tagged)-Human N-myc downstream regulated 1 (NDRG1), transcript variant 1



  "NM_001135242" in other vectors (6)

Reconstitution Protocol

USD 686.00


Availability*
4 Weeks

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NDRG1
Synonyms CAP43; CMT4D; DRG-1; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC225609 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCGGGAGATGCAGGATGTAGACCTCGCTGAGGTGAAGCCTTTGGTGGAGAAAGGGGAGACCATCA
CCGGCCTCCTGCAAGAGTTTGATGTCCAGGAGCAGGACATCGAGACTTTACATGGCTCTGTTCACGTCAC
GCTGTGTGGGACTCCCAAGGGAAACCGGCCTGTCATCCTCACCTACCATGACATCGGCATGAACCACAAA
ACCTGCTACAACCCCCTCTTCAACTACGAGGACATGCAGGAGATCACCCAGCACTTTGCCGTCTGCCACG
TGGACGCCCCTGGCCAGCAGGACGGCGCAGCCTCCTTCCCCGCAGGGTACATGTACCCCTCCATGGATCA
GCTGGCTGAAATGCTTCCTGGAGTCCTTCAACAGTTTGGGCTGAAAAGCATTATTGGCATGGGAACAGGA
GCAGGCGCCTACATCCTAACTCGATTTGCTCTAAACAACCCTGAGATGGTGGAGGGCCTTGTCCTTATCA
ACGTGAACCCTTGTGCGGAAGGCTGGATGGACTGGGCCGCCTCCAAGATCTCAGGATGGACCCAAGCTCT
GCCGGACATGGTGGTGTCCCACCTTTTTGGGAAGGAAGAAATGCAGAGTAACGTGGAAGTGGTCCACACC
TACCGCCAGCACATTGTGAATGACATGAACCCCGGCAACCTGCACCTGTTCATCAATGCCTACAACAGCC
GGCGCGACCTGGAGATTGAGCGACCAATGCCGGGAACCCACACAGTCACCCTGCAGTGCCCTGCTCTGTT
GGTGGTTGGGGACAGCTCGCCTGCAGTGGATGCCGTGGTGGAGTGCAACTCAAAATTGGACCCAACAAAG
ACCACTCTCCTCAAGATGGCGGACTGTGGCGGCCTCCCGCAGATCTCCCAGCCGGCCAAGCTCGCTGAGG
CCTTCAAGTACTTCGTGCAGGGCATGGGATACATGCCCTCGGCTAGCATGACCCGCCTGATGCGGTCCCG
CACAGCCTCTGGTTCCAGCGTCACTTCTCTGGATGGCACCCGCAGCCGCTCCCACACCAGCGAGGGCACC
CGAAGCCGCTCCCACACCAGCGAGGGCACCCGCAGCCGCTCGCACACCAGCGAGGGGGCCCACCTGGACA
TCACCCCCAACTCGGGTGCTGCTGGGAACAGCGCCGGGCCCAAGTCCATGGAGGTCTCCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC225609 protein sequence
Red=Cloning site Green=Tags(s)

MSREMQDVDLAEVKPLVEKGETITGLLQEFDVQEQDIETLHGSVHVTLCGTPKGNRPVILTYHDIGMNHK
TCYNPLFNYEDMQEITQHFAVCHVDAPGQQDGAASFPAGYMYPSMDQLAEMLPGVLQQFGLKSIIGMGTG
AGAYILTRFALNNPEMVEGLVLINVNPCAEGWMDWAASKISGWTQALPDMVVSHLFGKEEMQSNVEVVHT
YRQHIVNDMNPGNLHLFINAYNSRRDLEIERPMPGTHTVTLQCPALLVVGDSSPAVDAVVECNSKLDPTK
TTLLKMADCGGLPQISQPAKLAEAFKYFVQGMGYMPSASMTRLMRSRTASGSSVTSLDGTRSRSHTSEGT
RSRSHTSEGTRSRSHTSEGAHLDITPNSGAAGNSAGPKSMEVSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001135242
ORF Size 1182 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001135242.2
RefSeq Size 3520 bp
RefSeq ORF 1185 bp
Locus ID 10397
UniProt ID Q92597
Cytogenetics 8q24.22
MW 42.8 kDa
Gene Summary This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Other Versions

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