CNOT4 (NM_001008225) Human Untagged Clone

CAT#: SC324655

CNOT4 (untagged)-Human CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CNOT4
• Synonyms: CLONE243; NOT4; NOT4H
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001008225.1 CCACGCGTCCGGGTGGCCCGTCTGGCTGTGGAACCCACCGAAAGCTGAGAGTCTCTCTCT
  CAGCCGTAAAGGTCGGACTCGCTGAAGCAATCAGGCCCGGTTCTGAAGAGTTTTTTCCCC
  GACGCTCGTTCGGTGGTTTCTTGTGAGGACGCGGCAGCCCGTGGAGTCGGCTGAGGGAAA
  AGCGAGGCAAGGCAGGCGGGTCGTCAAAGTCCTACATTTTAGCTTTCAGTGCTGAACTTC
  CTTCTCCCTTAAATTATTATAAACTTTTGCTGCTGTTTAATAAACGTGAAGATGTCTCGC
  AGTCCTGATGCGAAGGAAGACCCTGTGGAGTGCCCTCTTTGCATGGAGCCCTTGGAGATA
  GATGATATCAACTTTTTCCCTTGCACCTGTGGCTACCAGATTTGCCGATTTTGTTGGCAT
  CGAATTCGCACTGATGAAAATGGGCTTTGTCCTGCATGTAGAAAGCCATATCCAGAAGAC
  CCAGCAGTTTATAAACCACTCTCCCAGGAAGAGCTGCAAAGGATAAAGAATGAGAAAAAA
  CAGAAACAAAATGAGAGAAAACAGAAAATATCAGAAAATCGCAAACATTTGGCTAGTGTA
  CGTGTCGTACAAAAAAACCTCGTCTTTGTTGTAGGTTTATCTCAGCGCCTAGCAGACCCA
  GAGGTTTTAAAACGACCAGAATATTTTGGGAAGTTTGGTAAAATACATAAAGTTGTCATC
  AATAATAGCACATCATATGCAGGCTCACAGGGTCCAAGTGCCAGTGCTTATGTAACCTAT
  ATCCGGTCAGAAGACGCTCTCAGAGCCATACAGTGTGTCAACAATGTGGTAGTAGATGGC
  AGAACACTTAAGGCATCTCTAGGTACAACAAAATACTGCAGTTACTTCTTAAAGAATATG
  CAGTGTCCAAAACCTGACTGCATGTATCTTCATGAATTGGGGGATGAGGCGGCCAGCTTC
  ACAAAAGAGGAAATGCAGGCGGGTAAACACCAAGAATATGAACAGAAGCTACTTCAAGAA
  TTATATAAATTAAATCCCAATTTTCTTCAGCTATCTACGGGTTCAGTTGATAAAAATAAG
  AACAAAGTGACACCACTGCAGAGCCCCATTGACAAACCTTCAGATTCTCTCAGTATAGGG
  AACGGTGATAATTCCCAGCAGATATCTAACAGTGATACGCCTTCACCACCACCTGGTTTG
  TCAAAATCCAATCCAGTCATCCCCATCAGTTCATCCAATCACAGTGCACGGTCCCCTTTT
  GAAGGGGCAGTAACAGAGTCACAGTCGTTATTCTCAGACATTTTTCGCCATCCCAACCCT
  ATCCCAAGTGGGCTTCCTCCTTTCCCCAGCTCCCCACAGACATCCAGTGACTGGCCTACA
  GCACCAGAACCACAGAGCCTCTTCACATCAGAAACAATCCCAGTATCATCCTCTACAGAC
  TGGCAAGCAGCTTTTGGCTTTGGTTCTTCTAAACAACCAGAGGATGACTTGGGTTTTGAT
  CCCTTCGATGTCACTCGAAAAGCCTTAGCAGACCTGATTGAGAAGGAACTGTCCGTTCAA
  GACCAACCTTCCCTTTCGCCCACATCTCTTCAGAACTCCTCTTCACACACTACAACCGCC
  AAAGGTCCAGGCTCTGGATTCCTGCATCCTGCTGCAGCTACAAATGCCAATTCTCTCAAT
  AGTACCTTTTCAGTCTTGCCCCAGAGGTTCCCTCAATTTCAGCAGCACCGAGCGGTTTAT
  AATTCATTCAGTTTTCCAGGCCAGGCAGCCCGCTATCCTTGGATGGCCTTTCCACGCAAT
  AGCATCATGCACTTGAACCACACAGCAAACCCCACCTCAAATAGTAATTTCTTGGACTTG
  AATCTCCCGCCACAGCACAACACAGGTCTGGGAGGGATCCCTGTAGCAGGGGAAGAAGAG
  GTGAAGGTTTCGACCATGCCACTGTCAACCTCTTCCCATTCATTACAACAAGGACAGCAG
  CCTACAAGTCTCCACACTACTGTGGCCTGACAACAGAACTGAGAGGAGAGGATTAGACTC
  TGGGGTGCTTGCATGGGCAACTGGATTTTTGCATGATTCCTTTATGATTTTGCTTTTAAT
  GTATACACCCAGAAGAGCCAATATAAACGTTCCTCATGCCTACAGCTAGCGTGTTACCTC
  ATAGTTGTTGAAGTATTTCTTCATTAGGCCTTTAACATAATTTATTAGTGTAATAATTTT
  GGCATTGTTTCTCATGAGTGATACTATTTCTAACTCATAAAAATAAACTTGTAGTACTAA
  GATCCCAGGTGAGATTAGCTAGAATTGTTGTTATTTTGGCTCTATTATTGAAAAATTATA
  CAAAGGTAATGGTAGTATCTTGTTTTCTGGTTTGTGAAAAAATAATATCCTAATCTCCTG
  AAAAATTTGCAAATTAACAAGATAATGTCTATTGCATTCTTTGAATACTTGAGAAGAAAG
  AATACATATAAAACAAAATTGGTGGTGTCATTTAATGCTCAGAAGGATATTGTTGGCGAT
  TGTTTTTAGTTGTCTAGATTTATATCCTATAATATGCATTACATGTGTAGAAACTGTTTT
  GTTCTCTCTCTTTTGCACTTTTGCACTTTCTTTTTGAATGGTTGTTAATTATGCATAGAT
  GGTTAAAAGATAATTATTGTAGCCTAAATGCAATATAATCTCTTTTCTAATGCACTGCCT
  AGTATACCAGGAAATGCCTCAAAAACAGAACTTCAGTTTATAGTTGACTTATCTGAAACT
  TGAATACGGAGTAAACATTGTATGTGAAAAGCAACCATAGTAGTGTAACATTGTTACTGA
  ACAAACAGTAATTCTGTGGGATATTGTTTTGGAAATTTCATTGTTTAATAATTAAAATGT
  GATAATAAGATAATATTATAAGTACCTGACCAGAATGAGACTAAGGCAGAAGAGAAGAGA
  TTGGATGTCCTCTAAAATTAGGCCACCTAAATTGTAACAGATAATTTTAATCAAGAGAAT
  AGTGGAGTACTTTATTAGGACATAATGTGAAGTGATATTTCTGAAAACACATTTCTTGAA
  ATAGTTTTAAAACTGATAATAAAGGAAAAGAAACTGTACTTAGTTTGGAGCAGTGGACAA
  AAGCTGATTCAGAAAACATGACCATATTCTGCCTACATGAAGCATCTGGGAAGAAAGGGA
  AAGTCACACCTCCTCTTGAGATTTTAACCATTATGATAAAAGGTATGCTTTAATTTGTTA
  AAAAAAAAAAGTATGAAAGGGGATAATGTGAATACAAAAAAGAAGCCTAGAGATTGAAAA
  TAATAATAATAGAACATGTTATTAGGAGATGGTACTTAATGTCATGTTGGAGTTGGAGTT
  TTCAAGGTTACCTCTATATAAAATTGGATTATTTTCTGTTAGATATAATGTGACAAATTA
  AATGACTTTATCCCAAGGTACAGTGAGGTAAACAAAAATCTATTCTTAGATATCCTTAGA
  TTTGGTTAACTGAGGCTGTAGAAGCAGCGAATTGTCTAGCCATTTAAGTTATTTTTGTAA
  AATTCAGCATAAACATCAATCTCTAATTTCTATCTAGAAGTGACTTTAAGACATTACAAG
  AACATTGCCAATTTGGAAAATTCCAATAAAATACTGTAGACTTATTAATATTGTAGACTT
  ATTATCAGCCTTTTACTATAGCTTAATTTAATTATGGTTTTCATTTTTACTGTAAGAGGC
  CAAGACTTCACTTATGTCTCCTGTGGAGCTGAGGCTCCTGGGCCTTGTTATTATAACAAA
  CTCTGGCTCCTTGTAATGATGCTTACTAGCTCAAAGCCTTCTCCAGCATTCAGTGTTAGC
  CTTTCATTGCCTGGATCTTCTTACAAAGCCATAGCAAGTGGTTAGTGCCATTTGCTCCTT
  GGTGTTGAGAGATATACCTGTTAAATGAGGCTTCTTTCCTTTCTCATTCATAGCCAGCGT
  GGAAAAATCATCAGGTAGTTTGCCCAGATTTGCCAGAGCCCTAAATAAAATGGCAAAACG
  ATCTCCCTGCCTGCTTCTCCTCCCCACCCCCACTTACATGCACACCGTAAATCAAGAAAT
  GCTTTTTGGGCAATTAGACTTTATCTTCTAAATTTTTGCATAGAATGGCTAAACATGATC
  TTTTGCCTCACTTTGTAATTCCTACACCTTCCTTGTAAGCTTCACCTTTTGCTTACCTAA
  TATTTTATTTAAACATGGTTTTTTAAAAAACCTGAAGCTTTAAAATCATAGTCATAATTA
  ATTTTACACTAAAGGACAGAAGAAATTATACAACACATAAAAGAAGCCACTACTTCACAT
  AGCAAGCAAATTGAAATCAAAATGACAGAAGAATGAGAAATTCTTTAAAAGCTTCAGGTT
  TTTTTTGTTGTTGTTGTTAAATTCCTGCATGATGCATCATGTGCAAGGGAAACTTAATAT
  ACTTTTACACCCTTAACCTTTATAAAGTGACTAGCTTTTCCCTTAACATAGGAATTTTTA
  ACCTGGAGCCCAGATTTTTGAGAGTACACACAAAATTGTGGGTTTGTGTGCATGTACATA
  TGTCCATTTTTTTCTAGGGAGAAGATCCATAGTTATTAGATTTGTGAAGGATTTGGTGAT
  CCCCTTCTCCCATCAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001008225
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008225.1, NP_001008226.1
• RefSeq Size: 2808 bp
• RefSeq ORF: 1719 bp
• Locus ID: 4850
• UniProt ID: O95628
• Cytogenetics: 7q33
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: RNA degradation
• Gene Summary: The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon and differs in the 3' UTR and coding sequence compared to variant 6. The resulting isoform (b) lacks an internal 3 aa segment and has a shorter and distinct C-terminus compared to isoform f. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.