HPGDS (NM_014485) Human Untagged Clone

CAT#: SC324532

HPGDS (untagged)-Human hematopoietic prostaglandin D synthase (HPGDS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HPGDS
• Synonyms: GSTS; GSTS1; GSTS1-1; PGD2; PGDS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014485.2 GGCACAGTCACATACCCAGGGATACAAGACACTGCAGACTCCCGAGAGACATAACACAGA
  ATTGCACCATGCCAAACTACAAACTCACTTATTTTAATATGAGGGGGAGAGCAGAAATTA
  TTCGTTACATATTTGCTTATTTGGACATACAGTATGAAGACCACAGAATAGAACAAGCTG
  ACTGGCCTGAAATCAAATCAACTCTCCCATTTGGAAAAATCCCCATTTTGGAAGTTGATG
  GACTTACTCTTCACCAGAGCCTAGCAATAGCAAGATATTTGACCAAAAACACAGATTTGG
  CTGGAAACACAGAAATGGAACAATGTCATGTTGATGCTATTGTGGACACTCTGGATGATT
  TCATGTCATGTTTTCCTTGGGCAGAGAAAAAGCAAGATGTGAAAGAGCAGATGTTCAATG
  AGCTGCTCACGTATAATGCGCCTCATCTTATGCAAGACTTGGACACATATTTAGGGGGGA
  GAGAATGGCTTATTGGTAACTCTGTAACTTGGGCAGACTTCTACTGGGAGATTTGCAGTA
  CCACACTTTTGGTCTTTAAGCCTGACCTGTTAGACAACCATCCAAGGCTGGTGACTTTAC
  GGAAGAAAGTCCAAGCCATTCCTGCCGTCGCTAACTGGATAAAACGAAGGCCCCAAACCA
  AACTCTAGCTGATCCATGTTGCCTTCAAGTTTGTTTTTCTCGGGGGCATCTCTCTCATCA
  GATAAGACAGCTACATCAGCCTGCCAGATAATCCACATGCTCCCTCCCCAGCTCCACTAA
  GATTTTCACTTTAGCCATATTCTGATTTTTAAAAAGGAAAATAAAAACAAATCTTTCTTC
  AGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_014485
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014485.2, NP_055300.1
• RefSeq Size: 1615 bp
• RefSeq ORF: 600 bp
• Locus ID: 27306
• UniProt ID: O60760
• Cytogenetics: 4q22.3
• Domains: GST_N, GST_C
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: Prostaglandin-D synthase is a sigma class glutathione-S-transferase family member. The enzyme catalyzes the conversion of PGH2 to PGD2 and plays a role in the production of prostanoids in the immune system and mast cells. The presence of this enzyme can be used to identify the differentiation stage of human megakaryocytes. [provided by RefSeq, Jul 2008]