ZGPAT (NM_001083113) Human Untagged Clone

CAT#: SC323980

ZGPAT (untagged)-Human zinc finger, CCCH-type with G patch domain (ZGPAT), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZGPAT
• Synonyms: GPATC6; GPATCH6; KIAA1847; ZC3H9; ZC3HDC9; ZIP
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001083113.1 CCACGCGTCCGGCGGCGGCGCTCGGGAGGAAGTGCCGATCGGCTGCTGGGGCGAAAAGGG
  GGCGCCGGGCCGCTCTAGCCGCCCTGGTCCAGCGCCTCCCTCTCTCAGCATGGACGAGGA
  GAGCCTGGAGTCGGCCTTGCAGACCTACCGTGCGCAGCTGCAGCAGGTGGAGCTGGCCTT
  GGGCGCCGGCCTGGATTCGTCTGAGCAGGCTGACCTGCGCCAGCTGCAGGGGGACCTGAA
  GGAGCTCATCGAGCTCACCGAGGCCAGCCTGGTGTCTGTCAGGAAGAGCAGGTTGTTGGC
  CGCGCTGGACGAAGAGCGCCCGGGCCGCCAGGAAGATGCTGAGTACCAGGCTTTCCGGGA
  GGCCATCACTGAGGCGGTGGAGGCACCAGCAGCGGCCCGTGGGTCCGGATCAGAGACCGT
  TCCTAAAGCAGAGGCGGGGCCAGAATCTGCGGCAGGTGGGCAGGAGGAGGAAGAGGGAGA
  GGACGAGGAAGAGCTGAGTGGGACAAAGGTGAGCGCGCCCTACTACAGCTCCTGGGGCAC
  TCTGGAGTATCACAACGCCATGGTGGTGGGAACGGAAGAGGCGGAGGATGGCTCGGCGGG
  TGTCCGTGTGCTTTACCTGTACCCCACTCACAAGTCTCTGAAGCCGTGCCCGTTCTTCCT
  GGAGGGAAAGTGCCGCTTTAAGGAGAACTGCAGGTTCTCCCATGGGCAGGTGGTCTCTCT
  GGATGAGCTGCGCCCCTTCCAGGACCCAGACCTGAGCTCCCTGCAGGCCGGCTCTGCGTG
  TCTGGCCAAGCACCAGGATGGCCTCTGGCACGCAGCACGCATCACCGATGTGGACAACGG
  CTACTACACAGTCAAGTTTGACTCGCTGCTGCTGAGGGAGGCCGTGGTGGAGGGGGACGG
  CATCCTGCCCCCACTGCGCACAGAGGCCACAGAGTCCGACTCAGACAGCGACGGTACGGG
  TGACTCCAGCTATGCCAGAGTGGTGGGGTCAGATGCTGTGGACTCTGGGACCTGCAGCTC
  TGCCTTTGCTGGCTGGGAGGTGCACACGCGAGGTATAGGCTCCAGACTCCTCACCAAGAT
  GGGCTATGAGTTTGGCAAGGGTTTGGGCCGACACGCGGAAGGCCGGGTGGAGCCCATCCA
  TGCTGTGGTGTTGCCTCGAGGGAAGTCGCTGGACCAGTGTGTGGAGACCCTGCAGAAGCA
  GACCAGGGTTGGCAAGGCTGGCACCAACAAGCCCCCCAGGTGCCGGGGAAGAGGGGCCAG
  GCCTGGGGGCCGCCCAGCTCCTCGGAATGTGTTTGACTTCCTCAATGAAAAGCTGCAAGG
  TCAGGCTCCTGGGGCCCTAGAAGCCGGGGCGGCCCCAGCGGGGAGGAGGAGCAAGGACAT
  GTACCATGCCAGCAAGAGTGCCAAGCGGGCCCTGAGCCTGCGGCTCTTCCAGACTGAGGA
  GAAGATCGAGCGAACCCAGCGGGACATCAGGAGCATCCAGGAGGCTCTCGCCCGCAACGC
  TGGCCGGCATAGCGTGGCGTCAGCCCAGCTGCAGGAGAAGCTGGCAGGAGCCCAGCGCCA
  GCTGGGGCAGCTCCGGGCTCAGGAAGCCGGCCTGCAGCAGGAGCAGAGGAAGGCAGACAC
  CCACAAGAAGATGACTGAGTTCTAGAGACCCCACAAGCACTATGGACGAAGCGTGGGACC
  CCAGCACGGGCTGCCCTCAGGAAGACCAGTGTTGCCCGAGGAGGGGCCGGCCTGCTGGCC
  TGGGGCGTGCGGACACTGCTGAGTGGAGACAGAGCTGCGGGGTCCCATCTGGACACTTAC
  TTGCCCACCTGCCAGTGTCTTGGGCATTTCCTTGGCAAGGACATTAAAGTGATTTCATCA
  CAGTGTCAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001083113
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001083113.1, NP_001076582.1
• RefSeq Size: 1925 bp
• RefSeq ORF: 1536 bp
• Locus ID: 84619
• UniProt ID: Q8N5A5
• Cytogenetics: 20q13.33
• Gene Summary: Transcription repressor that specifically binds the 5'-GGAG[GA]A[GA]A-3' consensus sequence. Represses transcription by recruiting the chromatin multiprotein complex NuRD to target promoters. Negatively regulates expression of EGFR, a gene involved in cell proliferation, survival and migration. Its ability to repress genes of the EGFR pathway suggest it may act as a tumor suppressor. Able to suppress breast carcinogenesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate in-frame splice site in the middle portion of the coding region, compared to variant 1. This results in a shorter protein (isoform c), compared to isoform a. Variants 3, 4 and 5 encode the same isoform (c).