EEF2K (NM_013302) Human Untagged Clone

CAT#: SC323690

EEF2K (untagged)-Kinase deficient mutant (K170M) of Human eukaryotic elongation factor-2 kinase (EEF2K)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EEF2K
• Synonyms: CaMKIII; eEF-2K; HSU93850
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013302, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGACGAAGATCTCATCTTCCGCCTGGAAGGCGTTGATGGCGGCCAGTCCCCCCGAGCTGGCCATG
  ATGGTGATTCTGATGGGGACAGCGACGATGAGGAAGGTTACTTCATCTGCCCCATCACGGATGACCCAAG
  CTCGAACCAGAATGTCAATTCCAAGGTTAATAAGTACTACAGCAACCTAACAAAAAGTGAGCGGTATAGC
  TCCAGCGGGTCCCCGGCAAACTCCTTCCACTTCAAGGAAGCCTGGAAGCACGCAATCCAGAAGGCCAAGC
  ACATGCCCGACCCCTGGGCTGAGTTCCACCTGGAAGATATTGCCACCGAACGTGCTACTCGACACAGGTA
  CAACGCCGTCACCGGGGAATGGCTGGATGATGAAGTTCTGATCAAGATGGCATCTCAGCCCTTCGGCCGA
  GGAGCAATGAGGGAGTGCTTCCGGACGAAGAAGCTCTCCAACTTCTTGCATGCCCAGCAGTGGAAGGGCG
  CCTCCAACTACGTGGCGAAGCGCTACATCGAGCCCGTAGACCGGGATGTGTACTTTGAGGACGTGCGTCT
  ACAGATGGAGGCCAAGCTCTGGGGGGAGGAGTATAATCGGCACAAGCCCCCCAAGCAGGTGGACATCATG
  CAGATGTGCATCATCGAGCTGAAGGACAGACCGGGCAAGCCCCTCTTCCACCTGGAGCACTACATCGAGG
  GCAAGTACATCAAGTACAACTCCAACTCTGGCTTTGTCCGCGATGACAACATCCGCCTGACGCCGCAGGC
  CTTCAGCCACTTCACTTTTGAGCGTTCCGGCCATCAGCTGATAGTGGTGGACATCCAGGGAGTTGGGGAT
  CTCTACACTGACCCACAGATCCACACGGAGACGGGCACTGACTTTGGAGACGGCAACCTAGGTGTCCGCG
  GGATGGCGCTCTTCTTCTACTCTCATGCCTGCAACCGGATTTGCGAGAGCATGGGCCTTGCTCCCTTTGA
  CCTCTCGCCCCGGGAGAGGGATGCAGTGAATCAGAACACCAAGCTGCTGCAATCAGCCAAGACCATCTTG
  AGAGGAACAGAGGAAAAATGTGGGAGCCCCCGAGTAAGGACCCTCTCTGGGAGCCGGCCACCCCTGCTCC
  GTCCCCTTTCAGAGAACTCTGGAGACGAGAACATGAGCGACGTGACCTTCGACTCTCTCCCTTCTTCCCC
  ATCTTCGGCCACACCACACAGCCAGAAGCTAGACCACCTCCATTGGCCAGTGTTCAGTGACCTCGATAAC
  ATGGCATCCAGAGACCATGATCATCTAGACAACCACCGGGAGTCTGAGAATAGTGGGGACAGCGGATACC
  CCAGTGAGAAGCGGGGTGAGCTGGATGACCCTGAGCCCCGAGAACATGGCCACTCATACAGTAATCGGAA
  GTACGAGTCTGACGAAGACAGCCTGGGCAGCTCTGGACGGGTATGTGTAGAGAAGTGGAATCTCCTCAAC
  TCCTCCCGCCTCCACCTGCCGAGGGCTTCGGCCGTGGCCCTGGAAGTGCAAAGGCTTAATGCTCTGGACC
  TCGAAAAGAAAATCGGGAAGTCCATTTTGGGGAAGGTCCATCTGGCCATGGTGCGCTACCACGAGGGTGG
  GCGCTTCTGCGAGAAGGGCGAGGAGTGGGACCAGGAGTCGGCTGTCTTCCACCTGGAGCACGCAGCCAAC
  CTGGGCGAGCTGGAGGCCATCGTGGGCCTGGGACTCATGTACTCGCAGTTGCCTCATCACATCCTAGCCG
  ATGTCTCTCTGAAGGAGACAGAAGAGAACAAAACCAAAGGATTTGATTACTTACTAAAGGCCGCTGAAGC
  TGGCGACAGGCAGTCCATGATCCTAGTGGCGCGAGCTTTTGACTCTGGCCAGAACCTCAGCCCGGACAGG
  TGCCAAGACTGGCTAGAGGCCCTGCACTGGTACAACACTGCCCTGGAGATGACGGACTGTGATGAGGGCG
  GTGAGTACGACGGAATGCAGGACGAGCCCCGGTACATGATGCTGGCCAGGGAGGCCGAGATGCTGTTCAC
  AGGAGGCTACGGGCTGGAGAAGGACCCGCAGAGATCAGGGGACTTGTATACCCAGGCAGCAGAGGCAGCG
  ATGGAAGCCATGAAGGGCCGACTGGCCAACCAGTACTACCAAAAGGCTGAAGAGGCCTGGGCCCAGATGG
  AGGAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_013302 unedited
  ACCGCTCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAACCAGAATGTCAA
  TTCCAAGGTTAATAAGTACTACAGCAACCTAACAAAAAGTGAGCGGTATAGCTCCAGCGGGTCCCCGGCA
  AACTCCTTCCACTTCAAGGAAGCCTGGAAGCACGCAATCCAGAAGGCCAAGCACATGCCCGACCCCTGGG
  CTGAGTTCCACCTGGAAGATATTGCCACCGAACGTGCTACTCGACACAGGTACAACGCCGTCACCGGGGA
  AATTGCTGGGATGATGAAGTTCTGATCAAGATGGCATCTCAGCCCTTCGCCCGGAGGAGCAATGAGTGAG
  TGCTTCCGGACGAAAGAAGCCTCTCCAACTTTCTTGCATGCCCCAGCAGTTGGAGGGAACCCTCCACTAC
  TTGGCCGATGCCGCTACATCGAACCCCGTAGAACGGGGATTGGTACTTGAAGGAGTGGCTTCACCGATTG
  GAGGGCCAAGCCTCGGGGGGAAGGAATTAAAATGGGCAAAAGCCCCAAGCCGGTTGACATATATGCAGTT
  GTGACTTCTGAGGTAAAGGACGACCGGCAAAGCCCCCTCTCACCCGGGAACATACTGAGGGCCGGATACT
  AGATCACATCTCAATCTGTGCTGTGCGCGAGAGACATTCCCGAGACGCAAGGCTTACGCATTCATTGAAC
  GTCGGCACTACGAATGTGTGAATCAGGATTGGACTCTACTGAACCAGTTCCACGACGCACTGACTTGAAC
  GACCAGTGCGAAGGCTTTTACCTAAGCGAAGAATCGGACCCTGACTGCCTGACTGCCGAAAAAC
  
  Kinase domain sequence

  >SC323690 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CRMATCKCGCCTTCGGCGAGGAGCATGAGTGAGTGCTTCCGGACGAAGAAGCTCTCCAACTTCTTGCATG
  CCCAGCAGTGGAAGGGAGCCTCCAACTACGTGGCGATGCGCTACATCGAGCCCGTAGACCGGGATGTGTA
  CTTTGAGGACGTGCGTCTACAGATGGAGGCCAAGCTCTGGGGGGAGGAGTATAATCGGCACAAGCCCCCC
  AAGCAGGTGGACATCATGCAGATGTGCATCATCGAGCTGAAGGAC
  
• Restriction Sites: Please inquire
• ACCN: NM_013302
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013302.2, NP_037434.1
• RefSeq Size: 3599 bp
• RefSeq ORF: 2178 bp
• Locus ID: 29904
• UniProt ID: O00418
• Cytogenetics: 16p12.2
• Domains: Alpha_kinase
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]