EEF2K (NM_013302) Human Tagged ORF Clone

CAT#: RG207496

EEF2K (tGFP-tagged) - Human eukaryotic elongation factor-2 kinase (EEF2K)

Product Images

Circular map for RG207496

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EEF2K
• Synonyms: CaMKIII; eEF-2K; HSU93850
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207496 representing NM_013302
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACGAAGATCTCATCTTCCGCCTGGAAGGCGTTGATGGCGGCCAGTCCCCCCGAGCTGGCCGTG
  ATGGTGATTCTGATGGGGACAGCGACGATGAGGAAGGTTACTTCATCTGCCCCATCACGGATGACCCAAG
  CTCGAACCAGAATGTCAATTCCAAGGTTAATAAGTACTACAGCAACCTAACAAAAAGTGAGCGGTATAGC
  TCCAGCGGGTCCCCGGCAAACTCCTTCCACTTCAAGGAAGCCTGGAAGCACGCAATCCAGAAGGCCAAGC
  ACATGCCCGACCCCTGGGCTGAGTTCCACCTGGAAGATATTGCCACCGAACGTGCTACTCGACACAGGTA
  CAACGCCGTCACCGGGGAATGGCTGGATGATGAAGTTCTGATCAAGATGGCATCTCAGCCCTTCGGCCGA
  GGAGCAATGAGGGAGTGCTTCCGGACGAAGAAGCTCTCCAACTTCTTGCATGCCCAGCAGTGGAAGGGAG
  CCTCCAACTACGTGGCGAAGCGCTACATCGAGCCCGTAGACCGGGATGTGTACTTTGAGGACGTGCGTCT
  ACAGATGGAGGCCAAGCTCTGGGGGGAGGAGTATAATCGGCACAAGCCCCCCAAGCAGGTGGACATCATG
  CAGATGTGCATCATCGAGCTGAAGGACAGACCGGGCAAGCCCCTCTTCCACCTGGAGCACTACATCGAGG
  GCAAGTACATCAAGTACAACTCCAACTCTGGCTTTGTCCGCGATGACAACATCCGCCTGACGCCGCAGGC
  CTTCAGCCACTTCACTTTTGAGCGTTCCGGCCATCAGCTGATAGTGGTGGACATCCAGGGAGTTGGGGAT
  CTCTACACTGACCCACAGATCCACACGGAGACGGGCACTGACTTTGGAGACGGCAACCTAGGTGTCCGCG
  GGATGGCGCTCTTCTTCTACTCTCATGCCTGCAACCGGATTTGCGAGAGCATGGGCCTTGCTCCCTTTGA
  CCTCTCGCCCCGGGAGAGGGATGCAGTGAATCAGAACACCAAGCTGCTGCAATCAGCCAAGACCATCTTG
  AGAGGAACAGAGGAAAAATGTGGGAGCCCCCGAGTAAGGACCCTCTCTGGGAGCCGGCCACCCCTGCTCC
  GTCCCCTTTCAGAGAACTCTGGAGACGAGAACATGAGCGACGTGACCTTCGACTCTCTCCCTTCTTCCCC
  ATCTTCGGCCACACCACACAGCCAGAAGCTAGACCACCTCCATTGGCCAGTGTTCAGTGACCTCGATAAC
  ATGGCATCCAGAGACCATGATCATCTAGACAACCACCGGGAGTCTGAGAATAGTGGGGACAGCGGATACC
  CCAGTGAGAAGCGGGGTGAGCTGGATGACCCTGAGCCCCGAGAACATGGCCACTCATACAGTAATCGGAA
  GTACGAGTCTGACGAAGACAGCCTGGGCAGCTCTGGACGGGTATGTGTAGAGAAGTGGAATCTCCTCAAC
  TCCTCCCGCCTCCACCTGCCGAGGGCTTCGGCCGTGGCCCTGGAAGTGCAAAGGCTTAATGCTCTGGACC
  TCGAAAAGAAAATCGGGAAGTCCATTTTGGGGAAGGTCCATCTGGCCATGGTGCGCTACCACGAGGGTGG
  GCGCTTCTGCGAGAAGGGCGAGGAGTGGGACCAGGAGTCGGCTGTCTTCCACCTGGAGCACGCAGCCAAC
  CTGGGCGAGCTGGAGGCCATCGTGGGCCTGGGACTCATGTACTCGCAGTTGCCTCATCACATCCTAGCCG
  ATGTCTCTCTGAAGGAGACAGAAGAGAACAAAACCAAAGGATTTGATTACTTACTAAAGGCCGCTGAAGC
  TGGCGACAGGCAGTCCATGATCCTAGTGGCGCGAGCTTTTGACTCTGGCCAGAACCTCAGCCCGGACAGG
  TGCCAAGACTGGCTAGAGGCCCTGCACTGGTACAACACTGCCCTGGAGATGACGGACTGTGATGAGGGCG
  GTGAGTACGACGGAATGCAGGACGAGCCCCGGTACATGATGCTGGCCAGGGAGGCCGAGATGCTGTTCAC
  AGGAGGCTACGGGCTGGAGAAGGACCCGCAGAGATCAGGGGACTTGTATACCCAGGCAGCAGAGGCAGCG
  ATGGAAGCCATGAAGGGCCGACTGGCCAACCAGTACTACCAAAAGGCTGAAGAGGCCTGGGCCCAGATGG
  AGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207496 representing NM_013302
  Red=Cloning site Green=Tags(s)
  
  MADEDLIFRLEGVDGGQSPRAGRDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYS
  SSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGR
  GAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIM
  QMCIIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGD
  LYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTIL
  RGTEEKCGSPRVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDN
  MASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLN
  SSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAAN
  LGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDR
  CQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAA
  MEAMKGRLANQYYQKAEEAWAQMEE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013302
• ORF Size: 2175 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013302.3, NP_037434.1
• RefSeq Size: 7412 bp
• RefSeq ORF: 2178 bp
• Locus ID: 29904
• UniProt ID: O00418
• Cytogenetics: 16p12.2
• Domains: Alpha_kinase
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]