GRK6 (NM_002082) Human Untagged Clone

CAT#: SC323679

GRK6 (untagged)-Kinase deficient mutant (K215M) of Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRK6
• Synonyms: GPRK6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323679 sequence for NM_002082 edited (data generated by NextGen Sequencing)
  ATGGAGCTCGAGAACATCGTAGCGAACACGGTGCTACTCAAGGCCCGGGAAGGTGGCGGT
  GGAAATCGCAAAGGCAAAAGCAAGAAATGGCGGCAGATGCTCCAGTTCCCTCACATCAGC
  CAGTGCGAAGAGCTGCGGCTCAGCCTCGAGCGTGACTATCACAGCCTGTGCGAGCGGCAG
  CCCATTGGGCGCCTGCTGTTCCGAGAGTTCTGTGCCACGAGGCCGGAGCTGAGCCGCTGC
  GTCGCCTTCCTGGATGGGGTGGCCGAGTATGAAGTGACCCCGGATGACAAGCGGAAGGCA
  TGTGGGCGGCAGCTAACGCAGAATTTTCTGAGCCACACGGGTCCTGACCTCATCCCTGAG
  GTCCCCCGGCAGCTGGTGACGAACTGCACCCAGCGGCTGGAGCAGGGTCCCTGCAAAGAC
  CTTTTCCAGGAACTCACCCGGCTGACCCACGAGTACCTGAGCGTGGCCCCTTTTGCCGAC
  TACCTCGACAGCATCTACTTCAACCGTTTCCTGCAGTGGAAGTGGCTGGAAAGGCAGCCA
  GTGACCAAAAACACCTTCAGGCAATACCGAGTCCTGGGCAAAGGTGGCTTTGGGGAGGTG
  TGCGCCTGCCAGGTGCGGGCCACAGGTAAGATGTATGCCTGCATGAAGCTAGAGAAAAAG
  CGGATCAAGAAGCGGAAAGGGGAGGCCATGGCGCTGAACGAGAAGCAGATCCTGGAGAAA
  GTGAACAGTAGGTTTGTAGTGAGCTTGGCCTACGCCTATGAGACCAAGGACGCGCTGTGC
  CTGGTGCTGACACTGATGAACGGGGGCGACCTCAAGTTCCACATCTACCACATGGGCCAG
  GCTGGCTTCCCCGAAGCGCGGGCCGTCTTCTACGCCGCCGAGATCTGCTGTGGCCTGGAG
  GACCTGCACCGGGAGCGCATCGTGTACAGGGACCTGAAGCCCGAGAACATCTTGCTGGAT
  GACCACGGCCACATCCGCATCTCTGACCTGGGACTAGCTGTGCATGTGCCCGAGGGCCAG
  ACCATCAAAGGGCGTGTGGGCACCGTGGGTTACATGGCTCCGGAGGTGGTGAAGAATGAA
  CGGTACACGTTCAGCCCTGACTGGTGGGCGCTCGGCTGCCTCCTGTACGAGATGATCGCA
  GGCCAGTCGCCCTTCCAGCAGAGGAAGAAGAAGATCAAGCGGGAGGAGGTGGAGCGGCTG
  GTGAAGGAGGTCCCCGAGGAGTATTCCGAGCGCTTTTCCCCGCAGGCCCGCTCACTTTGC
  TCACAGCTCCTCTGCAAGGACCCTGCCGAACGCCTGGGGTGTCGTGGGGGCAGTGCCCGC
  GAGGTGAAGGAGCACCCCCTCTTTAAGAAGCTGAACTTCAAGCGGCTGGGAGCTGGCATG
  CTGGAGCCGCCCTTCAAGCCTGACCCCCAGGCCATTTACTGCAAGGATGTTCTGGACATT
  GAACAGTTCTCTACGGTCAAGGGCGTGGAGCTGGAGCCTACCGACCAGGACTTCTACCAG
  AAGTTTGCCACAGGCAGTGTGCCCATCCCCTGGCAGAACGAGATGGTGGAGACCGAGTGC
  TTCCAGGAGCTGAATGTCTTTGGGCTGGATGGCTCAGTTCCCCCAGACCTGGACTGGAAG
  GGCCAGCCACCTGCACCTCCTAAAAAGGGACTGCTGCAGAGACTCTTCAGTCGCCAANNG
  ATTGCTGTGGAAACTGCAGCGACAGCGAGGAAGAGCTCCCCACCCGCCTCTAGCCCCCAG
  CCCGAGGCCCCCACCAGCAGTTGGCGGTAG
  
  Clone variation with respect to NM_002082.3
  644 a=>t;1392 g=>c;1566 a=>g;1678 a=>n;1679 g=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002082 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGGCCGGGGAGG
  CCGCGGCGCGGTCACTGCGAGCCGAGCCGAGCCGCGCCGAGCCGCGCCGATCGCCATCCGGCCTCGGCAC
  TCGCGCGCGATCCCGGCCGGCGGCGCGGCCCGGCGGGCCAGGCGGCGCCACAGCCCATGGAGCTCGAGAA
  CATCGTAGCGAACACGTGCTACTCAAGCCCCGGGAAGGTGGCGGGTGGAAATCGCAAAAGGCAAAAGCAA
  GAAATGGCGGGCAGAATGGCTTCCAGTTTCCCTCACATCAAGCCCAGTGCCGAAAAAGCTGCGCGGCTCC
  AGCCTCCGAGCGCGTGACTATATCACAGCTGGGGCCAAGCCGGCACCCAATTTGGCCCGCTGTGCTGGTT
  CGAAAGATTTTTGGGCCACAAGGCGGGACCCTAACCCCCCTGCTTCCCTTTTCTGGGATGGGGGGGGCCC
  AATTGAAATGAGCCCCGGAGGAAAAAGCCGGAAGGGTTTGGGGGGGGAACCTAACCCCAAATTTTTTCAA
  GCCAACAGGGCCCTGACCCCTACCCCGAGGGTCCCCCCGGCACTGGGGAAAAAAATGAAACAAAGGGGTG
  GGGCAAGGGGCCTGGCGAGAACATTTTTCCGAAAAATAAACGGGGGAAAAAAGAATACCCGAGCGTGGGG
  CCCTTTTTTCGCCAACCTCCGCCAGAAGCTTCTTTTTCCGCTTTTTTGCAGTGAAAATACTCGAAAAGAA
  GCCCCTGTGCAAAAAAACACTCTTGGCGGAACTACTCCTCTGCGAAAAAGGCTTTTTGGAAGAGTGTTCC
  TCCCATGCTGCCCACACAAAAAATATTATCCCTCTATCCACTATAAAACCATATTATCATACGATAAGAG
  GGGGTGCGTCACTGCGCATCACGACAAACATTGCTATA
  
  Kinase domain sequence

  >SC323679 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CTTYCGACMGTGMAAAACACCTTCAGGCATACCGAGTCCTGGGCAAAGGTGGCTTTGGGGAGGTGTGCGC
  CTGCCAGGTGCGGGCCACAGGTAAGATGTATGCCTGCATGAAGCTAGAGAAAAAGCGGATCAAGAAGCGG
  AAAGGGGAGGCCATGGCGCTGAACGAGAAGCAGATCCTGGAGAAAGTGAACAGTAGGTTTGTAGTGAGCT
  TGGCCTACGCCTATGAGACCAAGGACGCGCTGTGCCTGGTGCTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_002082
• Insert Size: 2880 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002082.2, NP_002073.2
• RefSeq Size: 2932 bp
• RefSeq ORF: 1770 bp
• Locus ID: 2870
• UniProt ID: P43250
• Cytogenetics: 5q35.3
• Domains: RGS, pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• Gene Summary: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (B).