GRK6 (NM_002082) Human Untagged Clone

CAT#: SC108487

GRK6 (untagged)-Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRK6
• Synonyms: GPRK6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002082 edited
  TCCGAGCGCTTTTCCCCGCAGGCCCGCTCACTTTGCTCACAGCTCCTCTGCAAGGACCCT
  GCCGAACGCCTGGGGTGTCGTGGGGGCAGTGCCCGCGAGGTGAAGGAGCACCCCCTCTTT
  AAGAAGCTGAACTTCAAGCGGCTGGGAGCTGGCATGCTGGAGCCGCCCTTCAAGCCTGAC
  CCCCAGGCCATTTACTGCAAGGATGTTCTGGACATTGAACAGTTCTCTACGGTCAAGGGC
  GTGGAGCTGGAGCCTACCGACCAGGACTTCTACCAGAAGTTTGCCACAGGCAGTGTGCCC
  ATCCCCTGGCAGAACGAGATGGTGGAGACCGAGTGCTTCCAGGAGCTGAATGTCTTTGGG
  CTGGATGGCTCAGTTCCCCCAGACCTGGACTGGAAGGGCCAGCCACCTGCACCTCCTAAA
  AAGGGACTGCTGCAGAGACTCTTCAGTCGCCAAGATTGCTGTGGAAACTGCAGCGACAGC
  GAGGAAGAGCTCCCCACCCGCCTCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002082 unedited
  ACCTCAGGATTTGTAACCGACTTACTATAGGCGGCCGCGCAATTCGCACGAGGGGAGGCC
  GGGGAGGCCGCGGCGCGGTCACTGCGAGCCGAGCCGAGCCGCGCCGAGCCGCGCCGATCG
  CCATCCGGCCTCGGCACTCGCGCGCGATCCCGGCCGGCGGCGCGGCCCGGCGGGCCAGGC
  GGCGCCACAGCCCATGGAGCTCGAGAACATCGTATCGAACACGGTGCTACTCAAGGCCCG
  GGAAGGTGGCGGTGGAAATCGCAAAGGCAAAAGCAAGAAATGGCGGCAGATGCTCCAGTT
  CCCTCACATCAGCCAGTGCGAAGAGCTGCGGCTCAGCCTCGAGCGTGACTATCACAGCCT
  GTGCGAGCGGCAGCCCATTGGGCGCCTGCTGTTCCGAGAGTTCTGTGCCACGAGGCCGGA
  GCTGAGCCGCTGCGTCGCCTTCCTGGATGGGGTGGCCGAGTATGAAGTGACCCCGGATGA
  CAAGCGGAAGGCATGTGGGCGGCAGCTAACGCAGAATTTTCTGAGCCACACGGGTCCTGA
  CCTCATCCCTGAGGTCCCCCGGCAGCTGGTGACGAACTGCACCCAGCGGCTGGAGCAGGG
  TCCCTGCAAAGACCTTTTCCAGGAACTCACCCGGCTGACCCACGAGTACCTGAGCGTGGC
  CCCTTTTGCCGACTACCTCGACAGCATCTACTTCAACCGTTTCCTGCAGTGGAAGTGGCT
  GGGAAAGCAGCCAGTGACCAAAAACACCTTCAGGCAATACCGAGTCCTGGGCAAAGTGGC
  TTTGGGGAAGTGTGCGCCTGCCAGGTGCGGGCCACAGGTAAGATGTATGCCTGCCAAGAA
  CCTAGAGAAAAGCCGATCAAGAAGCGCAAAGGGGAAGCCCATGCCGCTGTACGCAGAACC
  CAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002082 unedited
  CCTGTGGTCTTTGTTATGAGGGGAGTCCGCATGTTTGAGGTTGACCGGGATGCCCCACCC
  CCTCCTAGGGCTGGGCTTGGGCGTTTCTGAAAGAGTGTCTGCAGGACAGAGCCCAGTATC
  AGGGACCAGGCTGTGGGAGCTGGAAGGCAGAGGCAGACCTATCTGGGCCGGCCGGGGCAG
  TGGGCTGATGGGTATCACAGGCAGCCCAATCTCTGGCCACTTTGGCACAGGCGTAGGGCA
  TACAGAGGCCCCAGACCCAGCGCTGACAAGCACTGAGAAGGGACAGCTTTGGGCCCCAGA
  GGGTAGCAGCCTGAGCCCAGGGCAGCAGGGGGAGGCACGAGTCTTACTCAGAGGCCGGGG
  TGGCCAGCACAGTGCTGAAGGCTCAGGGGTGTGACCACCCTCAGCCATCCAAGCTGGGCC
  AGAGGAGCCTTGAGTTTGGCAGTGGCTCCTCTGTCAGCTCCCGCTCTCCCAGCCAGGGCT
  GGCTGCCCCCCAGAGCCCAAGCGGGGGCGCCAGGTCTGCCGCGGGAATCAACAATGCTCT
  GGTCGCTATATACATTCGCACCACTACATAACTACAGAAAGGCCCGAGCTCCCCGCGATA
  ACAACTGACCGGGACGCCCCTTCGGTCCATGTGTGGTTCCACGCCCCCGCCAGAACTCAC
  CCAGCCTATGCGGCTGATGGACGGTTTGACCCCCGCTACCGTTTTCCTCGTGCTGTCCAC
  GCCCCCCCTCCCGCGGATACCGGGCCCCATCCCCGGCAATCTGTCACCGCGCCAGTGGCG
  TTGCTCGCTTACACTCCCGCGGGGTAATCTACGTCGCCTTCCGCCGCCCCGGGTTTTCCC
  CCCCCTTTCTTCCCGCCCTCTTCTTCGTGCTCCCCCTTCTCGCCGTCCCTCCTTTGATGC
  CGTGCCCCGCTCTCCACCACGGACGGGCCCCGTCTCCGCGCTGTGTCATGATTCGGCCCC
  CCCCGCTTCCCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002082
• Insert Size: 2880 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002082.2, NP_002073.2
• RefSeq Size: 2932 bp
• RefSeq ORF: 1770 bp
• Locus ID: 2870
• UniProt ID: P43250
• Cytogenetics: 5q35.3
• Domains: RGS, pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• Gene Summary: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (B).