Eph receptor A4 (EPHA4) (NM_004438) Human Untagged Clone

CAT#: SC323668

EPHA4 (untagged)-Kinase deficient mutant (K653M) of Human EPH receptor A4 (EPHA4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Eph receptor A4
• Synonyms: EK8; HEK8; SEK; TYRO1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004438, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGGGATTTTCTATTTCGCCCTATTTTCGTGTCTCTTCGGGATTTGCGACGCTGTCACAGGTTCCA
  GGGTATACCCCGCGAATGAAGTTACCTTATTGGATTCCAGATCTGTTCAGGGAGAACTTGGGTGGATAGC
  AAGCCCTCTGGAAGGAGGGTGGGAGGAAGTGAGTATCATGGATGAAAAAAATACACCAATCCGAACCTAC
  CAAGTGTGCAATGTGATGGAACCCAGCCAGAATAACTGGCTACGAACTGATTGGATCACCCGAGAAGGGG
  CTCAGAGGGTGTATATTGAGATTAAATTCACCTTGAGGGACTGCAATAGTCTTCCGGGCGTCATGGGGAC
  TTGCAAGGAGACGTTTAACCTGTACTACTATGAATCAGACAACGACAAAGAGCGTTTCATCAGAGAGAAC
  CAGTTTGTCAAAATTGACACCATTGCTGCTGATGAGAGCTTCACCCAAGTGGACATTGGTGACAGAATCA
  TGAAGCTGAACACCGAGATCCGGGATGTAGGGCCATTAAGCAAAAAGGGGTTTTACCTGGCTTTTCAGGA
  TGTGGGGGCCTGCATCGCCCTGGTATCAGTCCGTGTGTTCTATAAAAAGTGTCCACTCACAGTCCGCAAT
  CTGGCCCAGTTTCCTGACACCATCACAGGGGCTGATACGTCTTCCCTGGTGGAAGTTCGAGGCTCCTGTG
  TCAACAACTCAGAAGAGAAAGATGTGCCAAAAATGTACTGTGGGGCAGATGGTGAATGGCTGGTACCCAT
  TGGCAACTGCCTATGCAACGCTGGGCATGAGGAGCGGAGCGGAGAATGCCAAGCTTGCAAAATTGGATAT
  TACAAGGCTCTCTCCACGGATGCCACCTGTGCCAAGTGCCCACCCCACAGCTACTCTGTCTGGGAAGGAG
  CCACCTCGTGCACCTGTGACCGAGGCTTTTTCAGAGCTGACAACGATGCTGCCTCTATGCCCTGCACCCG
  TCCACCATCTGCTCCCCTGAACTTGATTTCAAATGTCAACGAGACATCTGTGAACTTGGAATGGAGTAGC
  CCTCAGAATACAGGTGGCCGCCAGGACATTTCCTATAATGTGGTATGCAAGAAATGTGGAGCTGGTGACC
  CCAGCAAGTGCCGACCCTGTGGAAGTGGGGTCCACTACACCCCACAGCAGAATGGCTTGAAGACCACCAA
  AGTCTCCATCACTGACCTCCTAGCTCATACCAATTACACCTTTGAAATCTGGGCTGTGAATGGAGTGTCC
  AAATATAACCCTAACCCAGACCAATCAGTTTCTGTCACTGTGACCACCAACCAAGCAGCACCATCATCCA
  TTGCTTTGGTCCAGGCTAAAGAAGTCACAAGATACAGTGTGGCACTGGCTTGGCTGGAACCAGATCGGCC
  CAATGGGGTAATCCTGGAATATGAAGTCAAGTATTATGAGAAGGATCAGAATGAGCGAAGCTATCGTATA
  GTTCGGACAGCTGCCAGGAACACAGATATCAAAGGCCTGAACCCTCTCACTTCCTATGTTTTCCACGTGC
  GAGCCAGGACAGCAGCTGGCTATGGAGACTTCAGTGAGCCCTTGGAGGTTACAACCAACACAGTGCCTTC
  CCGGATCATTGGAGATGGGGCTAACTCCACAGTCCTTCTGGTCTCTGTCTCGGGCAGTGTGGTGCTGGTG
  GTAATTCTCATTGCAGCTTTTGTCATCAGCCGGAGACGGAGTAAATACAGTAAAGCCAAACAAGAAGCGG
  ATGAAGAGAAACATTTGAATCAAGGTGTAAGAACATATGTGGACCCCTTTACGTACGAAGATCCCAACCA
  AGCAGTGCGAGAGTTTGCCAAAGAAATTGACGCATCCTGCATTAAGATTGAAAAAGTTATAGGAGTTGGT
  GAATTTGGTGAGGTATGCAGTGGGCGTCTCAAAGTGCCTGGCAAGAGAGAGATCTGTGTGGCTATCAAGA
  CTCTGAAAGCTGGTTATACAGACAAACAGAGGAGAGACTTCCTGAGTGAGGCCAGCATCATGGGACAGTT
  TGACCATCCGAACATCATTCACTTGGAAGGCGTGGTCACTAAATGTAAACCAGTAATGATCATAACAGAG
  TACATGGAGAATGGCTCCTTGGATGCATTCCTCAGGAAAAATGATGGCAGATTTACAGTCATTCAGCTGG
  TGGGCATGCTTCGTGGCATTGGGTCTGGGATGAAGTATTTATCTGATATGAGCTATGTGCATCGTGATCT
  GGCCGCACGGAACATCCTGGTGAACAGCAACTTGGTCTGCAAAGTGTCTGATTTTGGCATGTCCCGAGTG
  CTTGAGGATGATCCGGAAGCAGCTTACACCACCAGGGGTGGCAAGATTCCTATCCGGTGGACTGCGCCAG
  AAGCAATTGCCTATCGTAAATTCACATCAGCAAGTGATGTATGGAGCTATGGAATCGTTATGTGGGAAGT
  GATGTCGTACGGGGAGAGGCCCTATTGGGATATGTCCAATCAAGATGTGATTAAAGCCATTGAGGAAGGC
  TATCGGTTACCCCCTCCAATGGACTGCCCCATTGCGCTCCACCAGCTGATGCTAGACTGCTGGCAGAAGG
  AGAGGAGCGACAGGCCTAAATTTGGGCAGATTGTCAACATGTTGGACAAACTCATCCGCAACCCCAACAG
  CTTGAAGAGGACAGGGACGGAGAGCTCCAGACCTAACACTGCCTTGTTGGATCCAAGCTCCCCTGAATTC
  TCTGCTGTGGTATCAGTGGGCGATTGGCTCCAGGCCATTAAAATGGACCGGTATAAGGATAACTTCACAG
  CTGCTGGTTATACCACACTAGAGGCTGTGGTGCACGTGAACCAGGAGGACCTGGCAAGAATTGGTATCAC
  AGCCATCACGCACCAGAATAAGATTTTGAGCAGTGTCCAGGCAATGCGAACCCAAATGCAGCAGATGCAC
  GGCAGAATGGTTCCCGTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004438 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAGCGTTGGCACC
  GGCGAACCATGGCTGGGATTTTCTATTTCGCCCTATTTTCGTGTCTCTTCGGGATTTGCGACGCTGTCAC
  AGGTTCCAGGGTATACCCCGCGAATGAAGTTACCTTATTGGATTCCAGATCTGTTCAGGGAGAACTTGGG
  TGGATAGCAAGCCCTCTGGAAGGGAGGGGTGGGAGGGAAGTGAGTATCATGGATGAAAAAAAATACACCA
  ATCCCGAACCTACCAAGTGTGGCAAATGTGATGGACCCCAGGCAAGAATTACCTGGGCTAACGGACCTGT
  GAATGGGATATCACCCCGAGAAAGGGCTCTCAAAGGGGTGTTATTTTGGAGAATAAAAATTCACCCTGAG
  AGGGACTGGCATTGTCCCTTCCGGCCCGTCTGGGGGACTTTGCAAGGAGACACGTTAAACTGGTCCTACT
  ATTTAATTCAAAACACCGACCAAAAAAGCTTTTCATTCCAAAAAAACCCATTTTGCCAAATTTCACCCCT
  TGGGGGGGTAAAAAGACTTTCCCCCAAGGGGACTTTGGGGAACAAAACCTAAAACCTAACCCCGATTCCG
  GGGGTGGGGGGCCTTTAACCAAAGGGGTTTACCCGGGTTTTTGAAGTGGGGGGGCCCCGCCCCCCCCTGT
  TATCCCCGGGGGTTTTTAAAAAAAGTGGCCCACCAACCCCATCATGGGCACTTTTTCGCGACCCCCCTCG
  GGGGGGGTAAACTTTCTCGTGGGAGATATTAGCGCCTCTGTGACACCCTACAAAAAAAAGTGTCCCCAAT
  ATCTCGCGGCAGAGAGTGATGGGTCTACTTCTGCTGCACCATCACTGCGTAGGAGAGAACGCAAGAAGAC
  ACTCTCGT
  
  Kinase domain sequence

  >SC323668 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CCRRTGRGCATCCTGCWTAGATTGAAAGTTATAGGAGTTGGTGAATTTGGTGAGGTATGCAGTGGGCGTC
  TCAAAGTGCCTGGCAAGAGAGAGATCTGTGTGGCTATCATGACTcTGAAAGCTGGTTATACAGACAAACA
  GAGGAGAGACTTCCTGAGTGAGGCCAGCATCATGGGACAGTTTGACCATCCGAACATCATTCACTTGGAA
  GGCGTGGTCACTAAATGTAAACCAGTAATGATCATAACAGAGTAC
  
• Restriction Sites: Please inquire
• ACCN: NM_004438
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004438.3, NP_004429.1
• RefSeq Size: 6364 bp
• RefSeq ORF: 2961 bp
• Locus ID: 2043
• UniProt ID: P54764
• Cytogenetics: 2q36.1
• Domains: pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.