Eph receptor A4(EPHA4) (NM_004438) Human Tagged ORF Clone

CAT#: RC211230

EPHA4 (Myc-DDK-tagged)-Human EPH receptor A4 (EPHA4)

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  • TrueORF®

USD 577


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Size
    • 10 ug

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Specifications

Product Data
Product Name Eph receptor A4(EPHA4) (NM_004438) Human Tagged ORF Clone
Symbol EPHA4
Synonyms HEK8; SEK; TYRO1
Vector pCMV6-Entry
Sequence Data
ORF Nucleotide Sequence
>RC211230 representing NM_004438
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGGATTTTCTATTTCGCCCTATTTTCGTGTCTCTTCGGGATTTGCGACGCTGTCACAGGTTCCA
GGGTATACCCCGCGAATGAAGTTACCTTATTGGATTCCAGATCTGTTCAGGGAGAACTTGGGTGGATAGC
AAGCCCTCTGGAAGGAGGGTGGGAGGAAGTGAGTATCATGGATGAAAAAAATACACCAATCCGAACCTAC
CAAGTGTGCAATGTGATGGAACCCAGCCAGAATAACTGGCTACGAACTGATTGGATCACCCGAGAAGGGG
CTCAGAGGGTGTATATTGAGATTAAATTCACCTTGAGGGACTGCAATAGTCTTCCGGGCGTCATGGGGAC
TTGCAAGGAGACGTTTAACCTGTACTACTATGAATCAGACAACGACAAAGAGCGTTTCATCAGAGAGAAC
CAGTTTGTCAAAATTGACACCATTGCTGCTGATGAGAGCTTCACCCAAGTGGACATTGGTGACAGAATCA
TGAAGCTGAACACCGAGATCCGGGATGTAGGGCCATTAAGCAAAAAGGGGTTTTACCTGGCTTTTCAGGA
TGTGGGGGCCTGCATCGCCCTGGTATCAGTCCGTGTGTTCTATAAAAAGTGTCCACTCACAGTCCGCAAT
CTGGCCCAGTTTCCTGACACCATCACAGGGGCTGATACGTCTTCCCTGGTGGAAGTTCGAGGCTCCTGTG
TCAACAACTCAGAAGAGAAAGATGTGCCAAAAATGTACTGTGGGGCAGATGGTGAATGGCTGGTACCCAT
TGGCAACTGCCTATGCAACGCTGGGCATGAGGAGCGGAGCGGAGAATGCCAAGCTTGCAAAATTGGATAT
TACAAGGCTCTCTCCACGGATGCCACCTGTGCCAAGTGCCCACCCCACAGCTACTCTGTCTGGGAAGGAG
CCACCTCGTGCACCTGTGACCGAGGCTTTTTCAGAGCTGACAACGATGCTGCCTCTATGCCCTGCACCCG
TCCACCATCTGCTCCCCTGAACTTGATTTCAAATGTCAACGAGACATCTGTGAACTTGGAATGGAGTAGC
CCTCAGAATACAGGTGGCCGCCAGGACATTTCCTATAATGTGGTATGCAAGAAATGTGGAGCTGGTGACC
CCAGCAAGTGCCGACCCTGTGGAAGTGGGGTCCACTACACCCCACAGCAGAATGGCTTGAAGACCACCAA
AGTCTCCATCACTGACCTCCTAGCTCATACCAATTACACCTTTGAAATCTGGGCTGTGAATGGAGTGTCC
AAATATAACCCTAACCCAGACCAATCAGTTTCTGTCACTGTGACCACCAACCAAGCAGCACCATCATCCA
TTGCTTTGGTCCAGGCTAAAGAAGTCACAAGATACAGTGTGGCACTGGCTTGGCTGGAACCAGATCGGCC
CAATGGGGTAATCCTGGAATATGAAGTCAAGTATTATGAGAAGGATCAGAATGAGCGAAGCTATCGTATA
GTTCGGACAGCTGCCAGGAACACAGATATCAAAGGCCTGAACCCTCTCACTTCCTATGTTTTCCACGTGC
GAGCCAGGACAGCAGCTGGCTATGGAGACTTCAGTGAGCCCTTGGAGGTTACAACCAACACAGTGCCTTC
CCGGATCATTGGAGATGGGGCTAACTCCACAGTCCTTCTGGTCTCTGTCTCGGGCAGTGTGGTGCTGGTG
GTAATTCTCATTGCAGCTTTTGTCATCAGCCGGAGACGGAGTAAATACAGTAAAGCCAAACAAGAAGCGG
ATGAAGAGAAACATTTGAATCAAGGTGTAAGAACATATGTGGACCCCTTTACGTACGAAGATCCCAACCA
AGCAGTGCGAGAGTTTGCCAAAGAAATTGACGCATCCTGCATTAAGATTGAAAAAGTTATAGGAGTTGGT
GAATTTGGTGAGGTATGCAGTGGGCGTCTCAAAGTGCCTGGCAAGAGAGAGATCTGTGTGGCTATCAAGA
CTCTGAAAGCTGGTTATACAGACAAACAGAGGAGAGACTTCCTGAGTGAGGCCAGCATCATGGGACAGTT
TGACCATCCGAACATCATTCACTTGGAAGGCGTGGTCACTAAATGTAAACCAGTAATGATCATAACAGAG
TACATGGAGAATGGCTCCTTGGATGCATTCCTCAGGAAAAATGATGGCAGATTTACAGTCATTCAGCTGG
TGGGCATGCTTCGTGGCATTGGGTCTGGGATGAAGTATTTATCTGATATGAGCTATGTGCATCGTGATCT
GGCCGCACGGAACATCCTGGTGAACAGCAACTTGGTCTGCAAAGTGTCTGATTTTGGCATGTCCCGAGTG
CTTGAGGATGATCCGGAAGCAGCTTACACCACCAGGGGTGGCAAGATTCCTATCCGGTGGACTGCGCCAG
AAGCAATTGCCTATCGTAAATTCACATCAGCAAGTGATGTATGGAGCTATGGAATCGTTATGTGGGAAGT
GATGTCGTACGGGGAGAGGCCCTATTGGGATATGTCCAATCAAGATGTGATTAAAGCCATTGAGGAAGGC
TATCGGTTACCCCCTCCAATGGACTGCCCCATTGCGCTCCACCAGCTGATGCTAGACTGCTGGCAGAAGG
AGAGGAGCGACAGGCCTAAATTTGGGCAGATTGTCAACATGTTGGACAAACTCATCCGCAACCCCAACAG
CTTGAAGAGGACAGGGACGGAGAGCTCCAGACCTAACACTGCCTTGTTGGATCCAAGCTCCCCTGAATTC
TCTGCTGTGGTATCAGTGGGCGATTGGCTCCAGGCCATTAAAATGGACCGGTATAAGGATAACTTCACAG
CTGCTGGTTATACCACACTAGAGGCTGTGGTGCACGTGAACCAGGAGGACCTGGCAAGAATTGGTATCAC
AGCCATCACGCACCAGAATAAGATTTTGAGCAGTGTCCAGGCAATGCGAACCCAAATGCAGCAGATGCAC
GGCAGAATGGTTCCCGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC211230 representing NM_004438
Red=Cloning site Green=Tags(s)

MAGIFYFALFSCLFGICDAVTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTY
QVCNVMEPSQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIREN
QFVKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRN
LAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEERSGECQACKIGY
YKALSTDATCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSS
PQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTKVSITDLLAHTNYTFEIWAVNGVS
KYNPNPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRI
VRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANSTVLLVSVSGSVVLV
VILIAAFVISRRRSKYSKAKQEADEEKHLNQGVRTYVDPFTYEDPNQAVREFAKEIDASCIKIEKVIGVG
EFGEVCSGRLKVPGKREICVAIKTLKAGYTDKQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITE
YMENGSLDAFLRKNDGRFTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGMSRV
LEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEG
YRLPPPMDCPIALHQLMLDCWQKERSDRPKFGQIVNMLDKLIRNPNSLKRTGTESSRPNTALLDPSSPEF
SAVVSVGDWLQAIKMDRYKDNFTAAGYTTLEAVVHVNQEDLARIGITAITHQNKILSSVQAMRTQMQQMH
GRMVPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_004438
ORF Size 2958 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_004438.1, NM_004438.2, NM_004438.3, NM_004438.4, NP_004429
RefSeq Size 6364
RefSeq ORF 2961
Locus ID 2043
Cytogenetics 2q36.1
Domains pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
Protein Families Protein Kinase, Transmembrane, Druggable Genome
Protein Pathways Axon guidance
MW 109.7 kDa
Gene Summary This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015].
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Citations