Eph receptor A4 (EPHA4) (NM_004438) Human Untagged Clone

CAT#: SC117354

EPHA4 (untagged)-Human EPH receptor A4 (EPHA4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Eph receptor A4
• Synonyms: EK8; HEK8; SEK; TYRO1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004438 edited
  ATGGCTGGGATTTTCTATTTCGCCCTATTTTCGTGTCTCTTCGGGATTTGCGACGCTGTC
  ACAGGTTCCAGGGTATACCCCGCGAATGAAGTTACCTTATTGGATTCCAGATCTGTTCAG
  GGAGAACTTGGGTGGATAGCAAGCCCTCTGGAAGGAGGGTGGGAGGAAGTGAGTATCATG
  GATGAAAAAAATACACCAATCCGAACCTACCAAGTGTGCAATGTGATGGAACCCAGCCAG
  AATAACTGGCTACGAACTGATTGGATCACCCGAGAAGGGGCTCAGAGGGTGTATATTGAG
  ATTAAATTCACCTTGAGGGACTGCAATAGTCTTCCGGGCGTCATGGGGACTTGCAAGGAG
  ACGTTTAACCTGTACTACTATGAATCAGACAACGACAAAGAGCGTTTCATCAGAGAGAAC
  CAGTTTGTCAAAATTGACACCATTGCTGCTGATGAGAGCTTCACCCAAGTGGACATTGGT
  GACAGAATCATGAAGCTGAACACCGAGATCCGGGATGTAGGGCCATTAAGCAAAAAGGGG
  TTTTACCTGGCTTTTCAGGATGTGGGGGCCTGCATCGCCCTGGTATCAGTCCGTGTGTTC
  TATAAAAAGTGTCCACTCACAGTCCGCAATCTGGCCCAGTTTCCTGACACCATCACAGGG
  GCTGATACGTCTTCCCTGGTGGAAGTTCGAGGCTCCTGTGTCAACAACTCAGAAGAGAAA
  GATGTGCCAAAAATGTACTGTGGGGCAGATGGTGAATGGCTGGTACCCATTGGCAACTGC
  CTATGCAACGCTGGGCATGAGGAGCGGAGCGGAGAATGCCAAGCTTGCAAAATTGGATAT
  TACAAGGCTCTCTCCACGGATGCCACCTGTGCCAAGTGCCCACCCCACAGCTACTCTGTC
  TGGGAAGGAGCCACCTCGTGCACCTGTGACCGAGGCTTTTTCAGAGCTGACAACGATGCT
  GCCTCCATGCCCTGCACCCGTCCACCATCTGCTCCCCTGAACTTGATTTCAAATGTCAAC
  GAGACATCTGTGAACTTGGAATGGAGTAGCCCTCAGAATACAGGTGGCCGCCAGGACATT
  TCCTATAATGTGGTATGCAAGAAATGTGGAGCTGGTGACCCCAGCAAGTGCCGACCCTGT
  GGAAGTGGGGTCCACTACACCCCACAGCAGAATGGCTTGAAGACCACCAAAGTCTCCATC
  ACTGACCTCCTAGCTCATACCAATTACACCTTTGAAATCTGGGCTGTGAATGGAGTGTCC
  AAATATAACCCTAACCCAGACCAATCAGTTTCTGTCACTGTGACCACCAACCAAGCAGCA
  CCATCATCCATTGCTTTGGTCCAGGCTAAAGAAGTCACAAGATACAGTGTGGCACTGGCT
  TGGCTGGAACCAGATCGGCCCAATGGGGTAATCCTGGAATATGAAGTCAAGTATTATGAG
  AAGGATCAGAATGAGCGAAGCTATCGTATAGTTCGGACAGCTGCCAGGAACACAGATATC
  AAAGGCCTGAACCCTCTCACTTCCTATGTTTTCCACGTGCGAGCCAGGACAGCAGCTGGC
  TATGGAGACTTCAGTGAGCCCTTGGAGGTTACAACCAACACAGTGCCTTCCCGGATCATT
  GGAGATGGGGCTAACTCCACAGTCCTTCTGGTCTCTGTCTCGGGCAGTGTGGTGCTGGTG
  GTAATTCTCATTGCAGCTTTTGTCATCAGCCGGAGACGGAGTAAATACAGTAAAGCCAAA
  CAAGAAGCGGATGAAGAGAAACATTTGAATCAAGGTGTAAGAACATATGTGGACCCCTTT
  ACGTACGAAGATCCCAACCAAGCAGTGCGAGAGTTTGCCAAAGAAATTGACGCATCCTGC
  ATTAAGATTGAAAAAGTTATAGGAGTTGGTGAATTTGGTGAGGTATGCAGTGGGCGTCTC
  AAAGTGCCTGGCAAGAGAGAGATCTGTGTGGCTATCAAGACTCTGAAAGCTGGTTATACA
  GACAAACAGAGGAGAGACTTCCTGAGTGAGGCCAGCATCATGGGACAGTTTGACCATCCG
  AACATCATTCACTTGGAAGGCGTGGTCACTAAATGTAAACCAGTAATGATCATAACAGAG
  TACATGGAGAATGGCTCCTTGGATGCATTCCTCAGGAAAAATGATGGCAGATTTACAGTC
  ATTCAGCTGGTGGGCATGCTTCGTGGCATTGGGTCTGGGATGAAGTATTTATCTGATATG
  AGCTATGTGCATCGTGATCTGGCCGCACGGAACATCCTGGTGAACAGCAACTTGGTCTGC
  AAAGTGTCTGATTTTGGCATGTCCCGAGTGCTTGAGGATGATCCGGAAGCAGCTTACACC
  ACCAGGGGTGGCAAGATTCCTATCCGGTGGACTGCGCCAGAAGCAATTGCCTATCGTAAA
  TTCACATCAGCAAGTGATGTATGGAGCTATGGAATCGTTATGTGGGAAGTGATGTCGTAC
  GGGGAGAGGCCCTATTGGGATATGTCCAATCAAGATGTGATTAAAGCCATTGAGGAAGGC
  TATCGGTTACCCCCTCCAATGGACTGCCCCATTGCGCTCCACCAGCTGATGCTAGACTGC
  TGGCAGAAGGAGAGGAGCGACAGGCCTAAATTTGGGCAGATTGTCAACATGTTGGACAAA
  CTCATCCGCAACCCCAACAGCTTGAAGAGGACAGGGACGGAGAGCTCCAGACCTAACACT
  GCCTTGTTGGATCCAAGCTCCCCTGAATTCTCTGCTGTGGTATCAGTGGGCGATTGGCTC
  CAGGCCATTAAAATGGACCGGTATAAGGATAACTTCACAGCTGCTGGTTATACCACACTA
  GAGGCTGTGGTGCACGTGAACCAGGAGGACCTGGCAAGAATTGGTATCACAGCCATCACG
  CACCAGAATAAGATTTTGAGCAGTGTCCAGGCAATGCGAACCCAAATGCAGCAGATGCAC
  GGCAGAATGGTTCCCGTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004438 unedited
  GTAATACGAACTTACTATAGGGCGGCCGCGAATTCGCACGAGGGCAGCGTTGGCACCGGC
  GAACCTGGCTGGGATTTTCTATTTCGCCCTATTTTCGTGTCTCTTCGGGATTTGCGACGC
  TGTCACAGGTTCCAGGGTATACCCCGCGAATGAAGTTACCTTATTGGATTCCAGATCTGT
  TCAGGGAGAACTTGGGTGGATAGCAAGCCCTCTGGAAGGAGGGTGGGAGGAAGTGAGTAT
  CATGGATGAAAAAAATACACCAATCCGAACCTACCAAGTGTGCAATGTGATGGAACCCAG
  CCAGAATAACTGGCTACGAACTGATTGGATCACCCGAGAAGGGGCTCAGAGGGTGTATAT
  TGAGATTAAATTCACCTTGAGGGACTGCAATAGTCTTCCGGGCGTCATGGGGACTTGCAA
  GGAGACGTTTAACCTGTACTACTATGAATCAGACAACGACAAAGAGCGTTTCATCAGAGA
  GAACCAGTTTGTCAAAATTGACACCATTGCTGCTGATGAGAGCTTCACCCAAGTGGACAT
  TGGTGACAGAATCATGAAGCTGAACACCGAGATCCGGGATGTAGGGCCATTAAGCAAAAA
  GGGGTTTTACCTGGCTNTTCANGATGTGGGGGCCTGCATCGCCCTGGTATCAGTCCGTGT
  GTTCTATAAAAAGTGTCCACTCACAGTCCGCAATCTGGCCCAGTTTCCTGACACCATCAC
  AGGGGCTGATACGCTCTCCCTGGGGGAAGTTCGAGGCTCCTGTGTCCACAACTCAAAGAG
  AAAGATGTGCCCAAATGTACTGGTGGGCAAATGGTGAAGGGCTGGACCCATTGACACTGC
  CTATNCACGCTGGCATGAGAGACGACGGAGATGCCAACTTGCAATTGGATTTCCAAGGCT
  TTTTCCGGATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004438 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTAATTTCAGAGGCGAAGA
  CGAAGTAAAAAAATGCAGTTCTTCAATTAAAGTGCATGGATGAGGTAAACTAATTTCAAG
  AGTTTTGAGTTTATTCAGTACTGGCTCAGACGGGAACCATTCTGCCGTGCATCTGCTGCA
  TTTGGGTTCGCATTGCCTGGACACTGCTCAAAATCTTATTCTGGTGCGTGATGGCTGTGA
  TACCAATTCTTGCCAGGTCCTCCTGGTTCACGTGCACCACAGCCTCTAGTGTGGTATAAC
  CAGCAGCTGTGAAGTTATCCTTATACCGGTCCATTTTAATGGCCTGGAGCCAATCGCCCA
  CTGATACCACAGCAGAGAATTCAGGGGAGCTTGGATCCAACAAGGCAGTGTTAGGTCTGG
  AGCTCTCCGTCCCTGTCCTCTTCAAGCTGTTGGGGTTGCGGATGATTTTGTCCAACATGT
  TGACAATCTGCCCAAATTTAGGCCTGTCGCTCCTCTCCTTCTGCCAGCAGTCTAGCATCA
  GCTGGTGGAGCGCAATGGGGCAGTCCATTGGAGGGGGTAACCGATAGCCTTCCTCAATGG
  CTTTAATCACATCTTGATTGGACATATCCCAATAGGGCCTCTCCCCGTACGACATCACTT
  CCCACATAACGATTCCATAGCTCCATACATCACTTGCTGATGTGAATTTACGATAGGCAA
  TTGCTTCTGGCGCAGTCCACCGGATAGGAATCTTGCCACCCCTGGTGGTGTAAGCTGCTT
  CCGGATCATCCCTCAGCACTCGGGACATGCCANAATCAGACACTTTGCAGACCAAGTTGC
  CTGTCACCAGGATGTTCCCGTGCGCCAGATCACGATGCACATAGCTCATATCAGAAAATA
  CTTCATCCAGNACCAATGCCACGAGCATGCCCACAGCTGATGACTGTAATCGCCATCTTT
  TTCCTGAGATGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004438
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004438.3, NP_004429.1
• RefSeq Size: 6364 bp
• RefSeq ORF: 2961 bp
• Locus ID: 2043
• UniProt ID: P54764
• Cytogenetics: 2q36.1
• Domains: pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.