MYLK3 (NM_182493) Human Untagged Clone

CAT#: SC323576

MYLK3 (untagged)-Kinase deficient mutant (K520M) of Human myosin light chain kinase 3 (MYLK3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYLK3
• Synonyms: caMLCK; MLCK; MLCK2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323576 sequence for NM_182493 edited (data generated by NextGen Sequencing)
  ATGTCAGGAACCTCCAAGGAGAGTCTGGGGCATGGGGGGCTGCCAGGGTTGGGCAAGACC
  TGCTTAACAACCATGGACACAAAGCTGAACATGCTGAACGAGAAGGTGGACCAGCTCCTG
  CACTTCCAAGAAGATGTCACAGAGAAGTTGCAGAGCATGTGCCGAGACATGGGCCACCTG
  GAGCGGGGCCTGCACAGGCTGGAGGCCTCCCGGGCACCGGGCCCGGGCGGGGCTGATGGG
  GTTCCCCACATTGACACCCAGGCTGGGTGGCCCGAGGTCCTGGAGCTGGTGAGGGCCATG
  CAGCAGGATGCGGCCCAGCACGGTGCCAGGCTGGAGGCCCTCTTCAGGATGGTGGCTGCG
  GTGGACAGGGCCATCGCTTTGGTGGGGGCCACGTTCCAGAAATCAAAGGTGGCGGATTTC
  CTCATGCAGGGGCGTGTGCCCTGGAGGAGAGGCAGCCCAGGTGACAGCCCTGAGGAGAAT
  AAAGAGCGAGTGGAAGAAGAGGGAGGAAAACCAAAGCATGTGCTGAGCACCAGTGGGTTG
  CAGTCTGATGCCAGGGAGCCTGGGGAAGAGAGCCAGAAGGCGGACGTGCTGGAGGGGACA
  GCGGAGAGGCTGCCCCCCATCAGAGCGTCAGGGCTGGGAGCTGACCCCGCCCAGGCAGTG
  GTCTCACCGGGCCAGGGAGATGGTGTTCCTGGCCCAGCCCAGGCATTCCCTGGCCACCTG
  CCCCTGCCCACAAAGGTGGAAGCCAAGGCTCCTGAGACACCCAGCGAGAACCTCAGGACT
  GGCCTGGAATTGGCTCCAGCACCCGGCAGGGTCAATGTGGTCTCCCCGAGCCTGGAGGTT
  GCACCAGGTGCAGGACAAGGAGCATCGTCCAGCAGGCCTGACCCTGAGCCCTTAGAGGAA
  GGCACGAGGCTGACTCCAGGGCCTGGCCCTCAGTGCCCAGGGCCTCCAGGGCTGCCAGCC
  CAGGCCAGGGCAACCCACAGTGGTGGAGAAACACCTCCAAGGATCTCCATCCACATACAA
  GAGATGGATACTCCTGGGGAGATGCTGATGACAGGCAGGGGCAGCCTTGGACCCACCCTC
  ACCACAGAGGCTCCAGCAGCTGCCCAGCCAGGCAAGCAGGGCCCACCTGGGACCGGGCGC
  TGCCTCCAAGCCCCTGGGACTGAGCCCGGAGAACAGACCCCTGAAGGAGCCAGAGAGCTC
  TCCCCGCTGCAGGAGAGCAGCAGCCCCGGGGGAGTGAAGGCAGAGGAGGAGCAAAGGGCT
  GGGGCCGAGCCTGGCACGAGACCAAGCTTGGCCAGGAGTGACGACAATGACCACGAGGTT
  GGGGCCCTGGGCCTGCAGCAGGGCAAAAGCCCAGGGGCGGGAAACCCTGAGCCTGAGCAG
  GACTGTGCAGCCAGGGCTCCGGTGAGAGCTGAAGCAGTAAGGAGGATGCCCCCAGGCGCC
  GAGGCTGGCAGCGTGGTTCTGGATGACAGTCCGGCCCCACCAGCTCCTTTTGAACACCGG
  GTAGTGAGCGTCAAGGAGACCTCCATCTCTGCGGGTTACGAGGTGTGCCAGCACGAAGTC
  TTGGGAGGGGGTCGGTTTGGCCAGGTCCACAGGTGCACAGAGAAGTCCACAGGCCTCCCA
  CTGGCTGCCAWGATCATCAAAGTGAAGAGCGCCAAGGACCGGGAGGACGTGAAGAACGAG
  ATCAACATCATGAACCAGCTCAGCCACGTGAACCTGATCCAGCTCTATGACGCCTTCGAG
  AGCAAGCACAGCTGCACCCTTGTCATGGAGTACGTGGACGGGGGTGAGCTCTTCGACCGG
  ATCACAGATGAGAAGTACCACCTGACTGAGCTGGATGTGGTCCTGTTCACCAGGCAGATC
  TGTGAGGGTGTGCATTACCTGCACCAGCACTACATCCTGCACCTGGACCTCAAGCCGGAG
  AACATATTGTGCGTCAATCAGACAGGACATCAAATTAAGATCATTGACTTTGGGCTGGCC
  AGAAGGTACAAGCCTCGAGAGAAGCTGAAGGTGAACTTCGGCACTCCTGAGTTCCTGGCC
  CCAGAAGTCGTCAATTATGAGTTTGTCTCATTCCCCACAGACATGTGGAGTGTGGGAGTC
  ATCACCTACATGCTACTCAGTGGCTTGTCCCCATTTCTAGGGGAAACAGATGCAGAGACC
  ATGAATTTCATTGTAAACTGTAGCTGGGATTTTGATGCTGACACCTTTGAAGGGCTCTCG
  GAGGAGGCCAAGGACTTTGTTTCCCGGTTGCTGGTCAAAGAGAAGAGCTGCAGAATGAGT
  GCCACACAGTGCCTGAAACACGAGTGGCTGAATAATTTGCCTGCCAAAGCTTCAAGATCC
  AAAACTCGTCTCAAATCCCAACTACTGCTGCAGAAATACATAGCTCAAAGAAAATGGAAG
  AAACATTTCTATGTGGTGACTGCTGCCAACAGGTTAAGGAAATTTCCAACTTCTCCCTAA
  
  Clone variation with respect to NM_182493.2
  538 g=>t;1631 a=>w
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_182493 unedited
  CACCCCCCCCGTTGAAACCCAAGGGCCGGGAGGCGTGGCAGAAAAAAGGTCTACACAAGCCGAGCTCGTT
  TAGGGAACCGAAAAAATTCCGTCCACCACTCACTATAGGGCGGGCGCCAATTCCGCACGAGGCTGACAGC
  AGTCTGTAAACTACGGAGGACAATGACCTTGCACACACCAATGCCTGAGTGAAAACCATGGGTCTGTGCC
  TCTCCTTATTCCCTCCTCATGCCCTTGTCCGCCCTTCCCACCGGTTTGGAACCTCCCAGAGAGTCTGGGG
  GCAGGGGGGCGCCGCCAGGGATGAGCAAAACCTGCTTAACACACTGGACCCAAAACTGAACTTGCTTATT
  GAATATGGTGAACCCCATCCCGCACTCCCAAAAAAATTTAAAACACCAATTTCCAAGACATGTCCCCAGA
  AATAGGCCCACGTGAACAGGGGCCTTGAAAGCCTTGAAAGACCCCCCCGGCACCGGGCCCCAGACAGGGA
  TAAAAGGGCTCCCCCAAAATGACACCAAACTAGGATGTTCCAGGTTCTCCAAACC
  
  Kinase domain sequence

  >SC323576 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AWATCTCTGCGGATMGAGGTGTGCCAGCACGAAGTCTTGGGAGGGGGTCGGTTTGGCCAGGTCCACAGGT
  GCACAGAGAAGTCCACAGGCCTCCCACTGGCTGCCATGATCATCAAAGTGAAGAGCGCCAAGGACCGGGA
  GGACGTGAAGAACGAGATCAACATCATGAACCAGCTCAGCCACGTGAACCTGATCCAGCTCTATGACGCC
  TTCGAGAGCAAGCACAGCTGCACCCTTGTCATGGAGTACGTGGAC
  
• Restriction Sites: Please inquire
• ACCN: NM_182493
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182493.1, NP_872299.1
• RefSeq Size: 2760 bp
• RefSeq ORF: 2388 bp
• Locus ID: 91807
• UniProt ID: Q32MK0
• Cytogenetics: 16q11.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Gene Summary: Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 [PubMed 18202317]).[supplied by OMIM, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).