MYLK3 (NM_182493) Human Tagged ORF Clone

CAT#: RG222246

MYLK3 (tGFP-tagged) - Human myosin light chain kinase 3 (MYLK3)

Product Images

Circular map for RG222246

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MYLK3
• Synonyms: caMLCK; MLCK; MLCK2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222246 representing NM_182493
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGAACCTCCAAGGAGAGTCTGGGGCATGGGGGGCTGCCAGGGTTGGGCAAGACCTGCTTAACAA
  CCATGGACACAAAGCTGAACATGCTGAACGAGAAGGTGGACCAGCTCCTGCACTTCCAAGAAGATGTCAC
  AGAGAAGTTGCAGAGCATGTGCCGAGACATGGGCCACCTGGAGCGGGGCCTGCACAGGCTGGAGGCCTCC
  CGGGCACCGGGCCCGGGCGGGGCTGATGGGGTTCCCCACATTGACACCCAGGCTGGGTGGCCCGAGGTCC
  TGGAGCTGGTGAGGGCCATGCAGCAGGATGCGGCCCAGCACGGTGCCAGGCTGGAGGCCCTCTTCAGGAT
  GGTGGCTGCGGTGGACAGGGCCATCGCTTTGGTGGGGGCCACGTTCCAGAAATCAAAGGTGGCGGATTTC
  CTCATGCAGGGGCGTGTGCCCTGGAGGAGAGGCAGCCCAGGTGACAGCCCTGAGGAGAATAAAGAGCGAG
  TGGAAGAAGAGGGAGGAAAACCAAAGCATGTGCTGAGCACCAGTGGGGTGCAGTCTGATGCCAGGGAGCC
  TGGGGAAGAGAGCCAGAAGGCGGACGTGCTGGAGGGGACAGCGGAGAGGCTGCCCCCCATCAGAGCGTCA
  GGGCTGGGAGCTGACCCCGCCCAGGCAGTGGTCTCACCGGGCCAGGGAGATGGTGTTCCTGGCCCAGCCC
  AGGCATTCCCTGGCCACCTGCCCCTGCCCACAAAGGTGGAAGCCAAGGCTCCTGAGACACCCAGCGAGAA
  CCTCAGGACTGGCCTGGAATTGGCTCCAGCACCCGGCAGGGTCAATGTGGTCTCCCCGAGCCTGGAGGTT
  GCACCAGGTGCAGGACAAGGAGCATCGTCCAGCAGGCCTGACCCTGAGCCCTTAGAGGAAGGCACGAGGC
  TGACTCCAGGGCCTGGCCCTCAGTGCCCAGGGCCTCCAGGGCTGCCAGCCCAGGCCAGGGCAACCCACAG
  TGGTGGAGAAACACCTCCAAGGATCTCCATCCACATACAAGAGATGGATACTCCTGGGGAGATGCTGATG
  ACAGGCAGGGGCAGCCTTGGACCCACCCTCACCACAGAGGCTCCAGCAGCTGCCCAGCCAGGCAAGCAGG
  GCCCACCTGGGACCGGGCGCTGCCTCCAAGCCCCTGGGACTGAGCCCGGAGAACAGACCCCTGAAGGAGC
  CAGAGAGCTCTCCCCGCTGCAGGAGAGCAGCAGCCCCGGGGGAGTGAAGGCAGAGGAGGAGCAAAGGGCT
  GGGGCCGAGCCTGGCACGAGACCAAGCTTGGCCAGGAGTGACGACAATGACCACGAGGTTGGGGCCCTGG
  GCCTGCAGCAGGGCAAAAGCCCAGGGGCGGGAAACCCTGAGCCTGAGCAGGACTGTGCAGCCAGGGCTCC
  GGTGAGAGCTGAAGCAGTAAGGAGGATGCCCCCAGGCGCCGAGGCTGGCAGCGTGGTTCTGGATGACAGT
  CCGGCCCCACCAGCTCCTTTTGAACACCGGGTAGTGAGCGTCAAGGAGACCTCCATCTCTGCGGGTTACG
  AGGTGTGCCAGCACGAAGTCTTGGGAGGGGGTCGGTTTGGCCAGGTCCACAGGTGCACAGAGAAGTCCAC
  AGGCCTCCCACTGGCTGCCAAGATCATCAAAGTGAAGAGCGCCAAGGACCGGGAGGACGTGAAGAACGAG
  ATCAACATCATGAACCAGCTCAGCCACGTGAACCTGATCCAGCTCTATGACGCCTTCGAGAGCAAGCACA
  GCTGCACCCTTGTCATGGAGTACGTGGACGGGGGTGAGCTCTTCGACCGGATCACAGATGAGAAGTACCA
  CCTGACTGAGCTGGATGTGGTCCTGTTCACCAGGCAGATCTGTGAGGGTGTGCATTACCTGCACCAGCAC
  TACATCCTGCACCTGGACCTCAAGCCGGAGAACATATTGTGCGTCAATCAGACAGGACATCAAATTAAGA
  TCATTGACTTTGGGCTGGCCAGAAGGTACAAGCCTCGAGAGAAGCTGAAGGTGAACTTCGGCACTCCTGA
  GTTCCTGGCCCCAGAAGTCGTCAATTATGAGTTTGTCTCATTCCCCACAGACATGTGGAGTGTGGGAGTC
  ATCACCTACATGCTACTCAGTGGCTTGTCCCCATTTCTAGGGGAAACAGATGCAGAGACCATGAATTTCA
  TTGTAAACTGTAGCTGGGATTTTGATGCTGACACCTTTGAAGGGCTCTCGGAGGAGGCCAAGGACTTTGT
  TTCCCGGTTGCTGGTCAAAGAGAAGAGCTGCAGAATGAGTGCCACACAGTGCCTGAAACACGAGTGGCTG
  AATAATTTGCCTGCCAAAGCTTCAAGATCCAAAACTCGTCTCAAATCCCAACTACTGCTGCAGAAATACA
  TAGCTCAAAGAAAATGGAAGAAACATTTCTATGTGGTGACTGCTGCCAACAGGTTAAGGAAATTTCCAAC
  TTCTCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222246 representing NM_182493
  Red=Cloning site Green=Tags(s)
  
  MSGTSKESLGHGGLPGLGKTCLTTMDTKLNMLNEKVDQLLHFQEDVTEKLQSMCRDMGHLERGLHRLEAS
  RAPGPGGADGVPHIDTQAGWPEVLELVRAMQQDAAQHGARLEALFRMVAAVDRAIALVGATFQKSKVADF
  LMQGRVPWRRGSPGDSPEENKERVEEEGGKPKHVLSTSGVQSDAREPGEESQKADVLEGTAERLPPIRAS
  GLGADPAQAVVSPGQGDGVPGPAQAFPGHLPLPTKVEAKAPETPSENLRTGLELAPAPGRVNVVSPSLEV
  APGAGQGASSSRPDPEPLEEGTRLTPGPGPQCPGPPGLPAQARATHSGGETPPRISIHIQEMDTPGEMLM
  TGRGSLGPTLTTEAPAAAQPGKQGPPGTGRCLQAPGTEPGEQTPEGARELSPLQESSSPGGVKAEEEQRA
  GAEPGTRPSLARSDDNDHEVGALGLQQGKSPGAGNPEPEQDCAARAPVRAEAVRRMPPGAEAGSVVLDDS
  PAPPAPFEHRVVSVKETSISAGYEVCQHEVLGGGRFGQVHRCTEKSTGLPLAAKIIKVKSAKDREDVKNE
  INIMNQLSHVNLIQLYDAFESKHSCTLVMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQH
  YILHLDLKPENILCVNQTGHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGV
  ITYMLLSGLSPFLGETDAETMNFIVNCSWDFDADTFEGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWL
  NNLPAKASRSKTRLKSQLLLQKYIAQRKWKKHFYVVTAANRLRKFPTSP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182493
• ORF Size: 2457 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182493.3
• RefSeq Size: 7998 bp
• RefSeq ORF: 2460 bp
• Locus ID: 91807
• UniProt ID: Q32MK0
• Cytogenetics: 16q11.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Gene Summary: Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 [PubMed 18202317]).[supplied by OMIM, Jul 2008]