DYRK2 (NM_003583) Human Untagged Clone

CAT#: SC323525

DYRK2 (untagged)-Kinase deficient mutant (K251M) of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DYRK2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323525 sequence for NM_003583 edited (data generated by NextGen Sequencing)
  ATGAATGATCACCTGCATGTCGGCAGCCACGCTCACGGACAGATCCAGGTTCAACAGTTG
  TTTGAGGATAACAGTAACAAGCGGACAGTGCTCACGACACAACCAAATGGGCTTACAACA
  GTGGGCAAAACGGGCTTGCCAGTGGTGCCAGAGCGGCAGCTGGACAGCATTCATAGACGG
  CAGGGGAGCTCCACCTCTCTAAAGTCCATGGAAGGCATGGGGAAGGTGAAAGCCACCCCC
  ATGACACCTGAACAAGCAATGAAGCAATACATGCAAAAACTCACAGCCTTCGAACACCAT
  GAGATTTTCAGCTACCCTGAAATATATTTCTTGGGTCTAAATGCTAAGAAGCGCCAGGGC
  ATGACAGGTGGGCCCAACAATGGTGGCTATGATGATGACCAGGGATCATATGTGCAGGTG
  CCCCACGATCACGTGGCTTACAGGTATGAGGTCCTCAAGGTCATTGGGAAGGGGAGCTTT
  GGGCAGGTGGTCAAGGCCTACGATCACAAAGTCCACCAGCACGTGGCCCTAATGATGGTG
  CGGAATGAGAAGCGCTTCCACCGGCAAGCAGCGGAGGAGATCCGAATCCTGGAACACCTG
  CGGAAGCAGGACAAGGATAACACAATGAATGTCATCCATATGCTGGAGAATTTCACCTTC
  CGCAACCACATCTGCATGACGTTTGAGCTGCTGAGCATGAACCTCTATGAGCTCATCAAG
  AAGAATAAATTCCAGGGCTTCAGTCTGCCTTTGGTTCGCAAGTTTGCCCACTCGATTCTG
  CAGTGCTTGGATGCTTTGCACAAAAACAGAATAATTCACTGTGACCTTAAGCCCGAGAAC
  ATTTTGTTAAAGCAGCAGGGTAGAAGCGGTATTAAAGTAATTGATTTTGGCTCCAGTTGT
  TACGAGCATCAGCGTGTCTACACGTACATCCAGTCGCGTTTTTACCGGGCTCCAGAAGTG
  ATCCTTGGGGCCAGGTATGGCATGCCCATTGATATGTGGAGCCTGGGCTGCATTTTAGCA
  GAGCTCCTGACGGGTTACCCCCTCTTGCCTGGGGAAGATGAAGGGGACCAGCTGGCCTGT
  ATGATTGAACTGTTGGGCATGCCCTCACAGAAACTGCTGGATGCATCCAAACGAGCCAAA
  AATTTTGTGAGCTCCAAGGGTTATCCCCGTTACTGCACTGTCACGACTCTCTCAGATGGC
  TCTGTGGTCCTAAACGGAGGCCGTTCCCGGAGGGGGAAACTGAGGGGCCCACCGGAGAGC
  AGAGAGTGGGGGAACGCGCTGAAGGGGTGTGATGATCCCCTTTTCCTTGACTTCTTAAAA
  CAGTGTTTAGAGTGGGATCCTGCAGTGCGCATGACCCCAGGCCAGGCTTTGCGGCACCCC
  TGGCTGAGGAGGCGGTTGCCAAAGCCTCCCACCGGGGAGAAAACGTCAGTGAAAAGGATA
  ACTGAGAGCACCGGTGCTATCACATCTATATCCAAGTTACCTCCACCTTCTAGCTCAGCT
  TCCAAACTGAGGACTAATTTGGCGCAGATGACAGATGCCAATGGGAATATTCAGCAGAGG
  ACAGTGTTGCCAAAACTTGTTAGCTGA
  
  Clone variation with respect to NM_003583.3
  533 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003583 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGCGCGCAGGGGA
  GGGCGAGGCATGTGCACGGGCCGGAGGGTGCTGCAGCCGCCCGAGGAAGAGGAGGACGGCGGCGAGGAGG
  AGAGCGGGGGGCTCGCGGCGGCGGGCCCCGGCCGAGGGGATGCAGTGGACTGTGTGTGTCTGGCTGTAGC
  AGACGCGAGGCGGCGACGAGGGCGCCGGGGACCCGCGCGAGGGGGCGGGCCGGGAGGCGGGCGGGCGGGC
  GGGCCGCCAGAAAGTAGCAGCAAGGACCGGGCGGCCGGGCGGACCGGCAAGCCCCTGGAAATGGCATTTT
  TCTTTCTCCCAGGCGGGCCATGTGTTACCCCAGGAAAACCTTTCGGCCGGCCGCTTCCCGCCCGCCTTAC
  CCGAACCGAATTGGCGGGCATTAAGCCCCACATGGAATAATCCCCTGATGTGTCGGCACCCACGCCTCAC
  GGCAGATATCCAGTTTCAAAAGTTTGTTTGGGGAAAACAGTAACAACGGACAGTGGGCTAGGACCCCACC
  AATGGGGCTCACCAAAGTGCAAAAACGGGTCTCCCAATGTGTGCCAGAGGGACCCTGGCACACATTATAG
  ACACGGCCGGGGGATCTCCCTCTCTCTAATGCACAGTGAAGCATATGAGAGAGAACCACACCCCTGTAGA
  CCCGTGCAACAATGAAGACATCATTGCGCAAACTCCAGGCTCGACACACTGAGATTTTAGCTCCTGAGAA
  TATATCTTGGGTAAGCTAGAAACCACGCATCACAGTGCACAATGGCTATGAGAACAGGATATTGCAGTGC
  CGATACTGCTCAGTAGATTTCAAGCATGTAGAGCCATGCAAGGCTGAAGGATCTC
  
  Kinase domain sequence

  >SC323525 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TCAGATMGTGGCTTAAGGTATGAGGTCCTCAAGGTCATTGGGAAGGGGAGCTTTGGGCAGGTGGTCAAGG
  CCTACGATCACAAAGTCCACCAGCACGTGGCCCTAATGATGGTGCGGAATGAGAAGCGCTTCCACCGGCA
  AGCAGCGGAGGAGATCCGAATCCTGGAACACCTGCGGAAGCAGGACAAGGATAACACAATGAATGTCATC
  CATATGCTGGAGAATTTCACCTTCCGCAACCACATCTGCATGACG
  
• Restriction Sites: Please inquire
• ACCN: NM_003583
• Insert Size: 5880 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003583.2, NP_003574.1
• RefSeq Size: 3466 bp
• RefSeq ORF: 1587 bp
• Locus ID: 8445
• UniProt ID: Q92630
• Cytogenetics: 12q15
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: DYRK2 belongs to a family of protein kinases whose members are presumed to be involved in cellular growth and/or development. The family is defined by structural similarity of their kinase domains and their capability to autophosphorylate on tyrosine residues. DYRK2 has demonstrated tyrosine autophosphorylation and catalyzed phosphorylation of histones H3 and H2B in vitro. Two isoforms of DYRK2 have been isolated. The predominant isoform, isoform 1, lacks a 5' terminal insert. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) does not include the 149 bp insert in the 5' end of the transcript and thus results in a shorter amino-terminal protein.