SPINK5 (NM_001127699) Human Untagged Clone

CAT#: SC323237

SPINK5 (untagged)-Human serine peptidase inhibitor, Kazal type 5 (SPINK5), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPINK5
• Synonyms: LEKTI; LETKI; NETS; NS; VAKTI
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001127699, the custom clone sequence may differ by one or more nucleotides
  ATGAAGATAGCCACAGTGTCAGTGCTTCTGCCCTTGGCTCTTTGCCTCATACAAGATGCT
  GCCAGTAAGAATGAAGATCAGGAAATGTGCCATGAATTTCAGGCATTTATGAAAAATGGA
  AAACTGTTCTGTCCCCAGGATAAGAAATTTTTTCAAAGTCTTGATGGAATAATGTTCATC
  AATAAATGTGCCACGTGCAAAATGATACTGGAAAAAGAAGCAAAATCACAGAAGAGGGCC
  AGGCATTTAGCAAGAGCTCCCAAGGCTACTGCCCCAACAGAGCTGAATTGTGATGATTTT
  AAAAAAGGAGAAAGAGATGGGGATTTTATCTGTCCTGATTATTATGAAGCTGTTTGTGGC
  ACAGATGGGAAAACATATGACAACAGATGTGCACTGTGTGCTGAGAATGCGAAAACCGGG
  TCCCAAATTGGTGTAAAAAGTGAAGGGGAATGTAAGAGCAGTAATCCAGAGCAGGATGTA
  TGCAGTGCTTTTCGGCCCTTTGTTAGAGATGGAAGACTTGGATGCACAAGGGAAAATGAT
  CCTGTTCTTGGTCCTGATGGGAAGACGCATGGCAATAAGTGTGCAATGTGTGCTGAGCTG
  TTTTTAAAAGAAGCTGAAAATGCCAAGCGAGAGGGTGAAACTAGAATTCGACGAAATGCT
  GAAAAGGATTTTTGCAAGGAATATGAAAAACAAGTGAGAAATGGAAGGCTTTTTTGTACA
  CGGGAGAGTGATCCAGTCCGTGGCCCTGACGGCAGGATGCATGGCAACAAATGTGCCCTG
  TGTGCTGAAATTTTCAAGCAGCGTTTTTCAGAGGAAAACAGTAAAACAGATCAAAATTTG
  GGAAAAGCTGAAGAAAAAACTAAAGTTAAAAGAGAAATTGTGAAACTCTGCAGTCAATAT
  CAAAATCAGGCAAAGAATGGAATACTTTTCTGTACCAGAGAAAATGACCCTATTCGTGGT
  CCAGATGGGAAAATGCATGGCAACTTGTGTTCCATGTGTCAAGCCTACTTCCAAGCAGAA
  AATGAAGAAAAGAAAAAGGCTGAAGCACGAGCTAGAAACAAAAGAGAATCTGGAAAAGCA
  ACCTCATATGCAGAGCTTTGCAGTGAATATCGAAAGCTTGTGAGGAACGGAAAACTTGCT
  TGCACCAGAGAGAACGATCCTATCCAGGGCCCAGATGGGAAAGTGCATGGCAACACCTGC
  TCCATGTGTGAGGTCTTCTTCCAAGCAGAAGAAGAAGAAAAGAAAAAGAAGGAAGGTAAA
  TCAAGAAACAAAAGACAATCTAAGAGTACAGCTTCCTTTGAGGAGTTGTGTAGTGAATAC
  CGCAAATCCAGGAAAAACGGACGGCTTTTTTGCACCAGAGAGAATGACCCCATCCAGGGC
  CCAGATGGAAAAATGCATGGCAACACCTGCTCCATGTGTGAGGCCTTCTTTCAACAAGAA
  GAAAGAGCAAGAGCAAAGGCTAAAAGAGAAGCTGCAAAGGAAATCTGCAGTGAATTTCGG
  GACCAAGTGAGGAATGGAACACTTATATGCACCAGGGAGCATAATCCTGTCCGTGGCCCA
  GATGGCAAAATGCATGGAAACAAGTGTGCCATGTGTGCCAGTGTGTTCAAACTTGAAGAA
  GAAGAGAAGAAAAATGATAAAGAAGAAAAAGGGAAAGTCGAGGCTGAAAAAGTTAAGAGA
  GAAGCAGTTCAGGAGCTGTGCAGTGAATATCGTCATTATGTGAGGAATGGACGACTCCCC
  TGTACCAGAGAGAATGATCCTATTGAGGGTCTAGATGGGAAAATCCACGGCAACACCTGC
  TCCATGTGTGAAGCCTTCTTCCAGCAAGAAGCAAAAGAAAAAGAAAGAGCTGAACCCAGA
  GCAAAAGTCAAAAGAGAAGCTGAAAAGGAGACATGCGATGAATTTCGGAGACTTTTGCAA
  AATGGAAAACTTTTCTGCACAAGAGAAAATGATCCTGTGCGTGGCCCAGATGGCAAGACC
  CATGGCAACAAGTGTGCCATGTGTAAGGCAGTCTTCCAGAAAGAAAATGAGGAAAGAAAG
  AGGAAAGAAGAGGAAGATCAGAGAAATGCTGCAGGACATGGTTCCAGTGGTGGTGGAGGA
  GGAAACACTCAGGACGAATGTGCTGAGTATCGGGAACAAATGAAAAATGGAAGACTCAGC
  TGTACTCGGGAGAGTGATCCTGTACGTGATGCTGATGGCAAATCGTACAACAATCAGTGT
  ACCATGTGTAAAGCAAAATTGGAAAGAGAAGCAGAGAGAAAAAATGAGTATTCTCGCTCC
  AGATCAAATGGGACTGGATCAGAATCAGGGAAGGATACATGTGATGAGTTTAGAAGCCAA
  ATGAAAAATGGAAAACTCATCTGCACTCGAGAAAGTGACCCTGTCCGGGGTCCAGATGGC
  AAGACACATGGCAATAAGTGTACTATGTGTAAGGAAAAACTGGAAAGGGAAGCAGCTGAA
  AAAAAAAAGAAAGAGGATGAAGACAGGAGCAATACAGGAGAAAGGAGCAATACAGGAGAA
  AGGAGCAATGACAAAGAGGATCTGTGTCGTGAATTTCGAAGCATGCAGAGAAATGGAAAG
  CTTATCTGCACCAGAGAAAATAACCCTGTTCGAGGCCCATATGGCAAGATGCACATCAAT
  AAATGTGCTATGTGTCAGAGCATCTTTGATCGAGAAGCTAATGAAAGAAAAAAGAAAGAT
  GAAGAGAAATCAAGTAGCAAGCCCTCAAATAATGCAAAGGTTATTTAT
  
• Restriction Sites: Please inquire
• ACCN: NM_001127699
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127699.1, NP_001121171.1
• RefSeq Size: 2915 bp
• RefSeq ORF: 2751 bp
• Locus ID: 11005
• UniProt ID: Q9NQ38
• Cytogenetics: 5q32
• Gene Summary: This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (3, also known as SPINK5sh) differs in the 3' UTR and lacks several exons in the 3' coding region, compared to variant 1. The resulting protein (isoform c, also known as the LEKTIsh or short isoform) has a distinct C-terminus and is shorter than isoform a.