SPINK5 (NM_001127699) Human Tagged ORF Clone

CAT#: RG226261

SPINK5 (tGFP-tagged) - Human serine peptidase inhibitor, Kazal type 5 (SPINK5), transcript variant 3

Product Images

Circular map for RG226261

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SPINK5
• Synonyms: LEKTI; LETKI; NETS; NS; VAKTI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226261 representing NM_001127699
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATAGCCACAGTGTCAGTGCTTCTGCCCTTGGCTCTTTGCCTCATACAAGATGCTGCCAGTAAGA
  ATGAAGATCAGGAAATGTGCCATGAATTTCAGGCATTTATGAAAAATGGAAAACTGTTCTGTCCCCAGGA
  TAAGAAATTTTTTCAAAGTCTTGATGGAATAATGTTCATCAATAAATGTGCCACGTGCAAAATGATACTG
  GAAAAAGAAGCAAAATCACAGAAGAGGGCCAGGCATTTAGCAAGAGCTCCCAAGGCTACTGCCCCAACAG
  AGCTGAATTGTGATGATTTTAAAAAAGGAGAAAGAGATGGGGATTTTATCTGTCCTGATTATTATGAAGC
  TGTTTGTGGCACAGATGGGAAAACATATGACAACAGATGTGCACTGTGTGCTGAGAATGCGAAAACCGGG
  TCCCAAATTGGTGTAAAAAGTGAAGGGGAATGTAAGAGCAGTAATCCAGAGCAGGATGTATGCAGTGCTT
  TTCGGCCCTTTGTTAGAGATGGAAGACTTGGATGCACAAGGGAAAATGATCCTGTTCTTGGTCCTGATGG
  GAAGACGCATGGCAATAAGTGTGCAATGTGTGCTGAGCTGTTTTTAAAAGAAGCTGAAAATGCCAAGCGA
  GAGGGTGAAACTAGAATTCGACGAAATGCTGAAAAGGATTTTTGCAAGGAATATGAAAAACAAGTGAGAA
  ATGGAAGGCTTTTTTGTACACGGGAGAGTGATCCAGTCCGTGGCCCTGACGGCAGGATGCATGGCAACAA
  ATGTGCCCTGTGTGCTGAAATTTTCAAGCAGCGTTTTTCAGAGGAAAACAGTAAAACAGATCAAAATTTG
  GGAAAAGCTGAAGAAAAAACTAAAGTTAAAAGAGAAATTGTGAAACTCTGCAGTCAATATCAAAATCAGG
  CAAAGAATGGAATACTTTTCTGTACCAGAGAAAATGACCCTATTCGTGGTCCAGATGGGAAAATGCATGG
  CAACTTGTGTTCCATGTGTCAAGCCTACTTCCAAGCAGAAAATGAAGAAAAGAAAAAGGCTGAAGCACGA
  GCTAGAAACAAAAGAGAATCTGGAAAAGCAACCTCATATGCAGAGCTTTGCAGTGAATATCGAAAGCTTG
  TGAGGAACGGAAAACTTGCTTGCACCAGAGAGAACGATCCTATCCAGGGCCCAGATGGGAAAGTGCATGG
  CAACACCTGCTCCATGTGTGAGGTCTTCTTCCAAGCAGAAGAAGAAGAAAAGAAAAAGAAGGAAGGTAAA
  TCAAGAAACAAAAGACAATCTAAGAGTACAGCTTCCTTTGAGGAGTTGTGTAGTGAATACCGCAAATCCA
  GGAAAAACGGACGGCTTTTTTGCACCAGAGAGAATGACCCCATCCAGGGCCCAGATGGAAAAATGCATGG
  CAACACCTGCTCCATGTGTGAGGCCTTCTTTCAACAAGAAGAAAGAGCAAGAGCAAAGGCTAAAAGAGAA
  GCTGCAAAGGAAATCTGCAGTGAATTTCGGGACCAAGTGAGGAATGGAACACTTATATGCACCAGGGAGC
  ATAATCCTGTCCGTGGCCCAGATGGCAAAATGCATGGAAACAAGTGTGCCATGTGTGCCAGTGTGTTCAA
  ACTTGAAGAAGAAGAGAAGAAAAATGATAAAGAAGAAAAAGGGAAAGTCGAGGCTGAAAAAGTTAAGAGA
  GAAGCAGTTCAGGAGCTGTGCAGTGAATATCGTCATTATGTGAGGAATGGACGACTCCCCTGTACCAGAG
  AGAATGATCCTATTGAGGGTCTAGATGGGAAAATCCACGGCAACACCTGCTCCATGTGTGAAGCCTTCTT
  CCAGCAAGAAGCAAAAGAAAAAGAAAGAGCTGAACCCAGAGCAAAAGTCAAAAGAGAAGCTGAAAAGGAG
  ACATGCGATGAATTTCGGAGACTTTTGCAAAATGGAAAACTTTTCTGCACAAGAGAAAATGATCCTGTGC
  GTGGCCCAGATGGCAAGACCCATGGCAACAAGTGTGCCATGTGTAAGGCAGTCTTCCAGAAAGAAAATGA
  GGAAAGAAAGAGGAAAGAAGAGGAAGATCAGAGAAATGCTGCAGGACATGGTTCCAGTGGTGGTGGAGGA
  GGAAACACTCAGGACGAATGTGCTGAGTATCGGGAACAAATGAAAAATGGAAGACTCAGCTGTACTCGGG
  AGAGTGATCCTGTACGTGATGCTGATGGCAAATCGTACAACAATCAGTGTACCATGTGTAAAGCAAAATT
  GGAAAGAGAAGCAGAGAGAAAAAATGAGTATTCTCGCTCCAGATCAAATGGGACTGGATCAGAATCAGGG
  AAGGATACATGTGATGAGTTTAGAAGCCAAATGAAAAATGGAAAACTCATCTGCACTCGAGAAAGTGACC
  CTGTCCGGGGTCCAGATGGCAAGACACATGGCAATAAGTGTACTATGTGTAAGGAAAAACTGGAAAGGGA
  AGCAGCTGAAAAAAAAAAGAAAGAGGATGAAGACAGGAGCAATACAGGAGAAAGGAGCAATACAGGAGAA
  AGGAGCAATGACAAAGAGGATCTGTGTCGTGAATTTCGAAGCATGCAGAGAAATGGAAAGCTTATCTGCA
  CCAGAGAAAATAACCCTGTTCGAGGCCCATATGGCAAGATGCACATCAATAAATGTGCTATGTGTCAGAG
  CATCTTTGATCGAGAAGCTAATGAAAGAAAAAAGAAAGATGAAGAGAAATCAAGTAGCAAGCCCTCAAAT
  AATGCAAAGGTTATTTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226261 representing NM_001127699
  Red=Cloning site Green=Tags(s)
  
  MKIATVSVLLPLALCLIQDAASKNEDQEMCHEFQAFMKNGKLFCPQDKKFFQSLDGIMFINKCATCKMIL
  EKEAKSQKRARHLARAPKATAPTELNCDDFKKGERDGDFICPDYYEAVCGTDGKTYDNRCALCAENAKTG
  SQIGVKSEGECKSSNPEQDVCSAFRPFVRDGRLGCTRENDPVLGPDGKTHGNKCAMCAELFLKEAENAKR
  EGETRIRRNAEKDFCKEYEKQVRNGRLFCTRESDPVRGPDGRMHGNKCALCAEIFKQRFSEENSKTDQNL
  GKAEEKTKVKREIVKLCSQYQNQAKNGILFCTRENDPIRGPDGKMHGNLCSMCQAYFQAENEEKKKAEAR
  ARNKRESGKATSYAELCSEYRKLVRNGKLACTRENDPIQGPDGKVHGNTCSMCEVFFQAEEEEKKKKEGK
  SRNKRQSKSTASFEELCSEYRKSRKNGRLFCTRENDPIQGPDGKMHGNTCSMCEAFFQQEERARAKAKRE
  AAKEICSEFRDQVRNGTLICTREHNPVRGPDGKMHGNKCAMCASVFKLEEEEKKNDKEEKGKVEAEKVKR
  EAVQELCSEYRHYVRNGRLPCTRENDPIEGLDGKIHGNTCSMCEAFFQQEAKEKERAEPRAKVKREAEKE
  TCDEFRRLLQNGKLFCTRENDPVRGPDGKTHGNKCAMCKAVFQKENEERKRKEEEDQRNAAGHGSSGGGG
  GNTQDECAEYREQMKNGRLSCTRESDPVRDADGKSYNNQCTMCKAKLEREAERKNEYSRSRSNGTGSESG
  KDTCDEFRSQMKNGKLICTRESDPVRGPDGKTHGNKCTMCKEKLEREAAEKKKKEDEDRSNTGERSNTGE
  RSNDKEDLCREFRSMQRNGKLICTRENNPVRGPYGKMHINKCAMCQSIFDREANERKKKDEEKSSSKPSN
  NAKVIY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127699
• ORF Size: 2748 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127699.2
• RefSeq Size: 2915 bp
• RefSeq ORF: 2751 bp
• Locus ID: 11005
• UniProt ID: Q9NQ38
• Cytogenetics: 5q32
• Gene Summary: This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]