ATP6V0A1 (NM_001130021) Human Untagged Clone

CAT#: SC323217

ATP6V0A1 (untagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a1 (ATP6V0A1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP6V0A1
• Synonyms: a1; ATP6N1; ATP6N1A; Stv1; Vph1; VPP1
• Vector: pCMV6-AC-Myc-DDK
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001130021, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGGAGCTTTTCCGGAGTGAAGAAATGACACTGGCCCAGCTTTTTCTACAGTCAGAGGCTGCTTATT
  GTTGTGTCAGTGAATTAGGAGAACTTGGAAAGGTTCAGTTTCGTGACTTAAATCCAGATGTGAATGTTTT
  CCAACGGAAATTTGTGAATGAAGTTAGAAGATGTGAAGAAATGGATCGAAAGCTTCGATTTGTTGAGAAA
  GAGATAAGAAAAGCTAACATTCCGATTATGGACACCGGTGAAAACCCAGAGGTTCCCTTCCCCCGGGACA
  TGATTGACTTAGAGGCCAATTTTGAGAAGATTGAAAATGAACTGAAGGAAATCAACACAAACCAGGAAGC
  TCTGAAGAGAAACTTCCTGGAACTGACCGAATTAAAATTTATACTTCGCAAAACTCAGCAATTTTTTGAT
  GAGATGGCGGATCCAGACTTGTTGGAAGAGTCCTCATCCCTCTTGGAGCCAAGTGAGATGGGAAGAGGCA
  CTCCTTTAAGACTTGGCTTCGTGGCTGGTGTCATTAACCGGGAGCGCATCCCTACTTTTGAGCGCATGCT
  TTGGCGGGTATGCCGGGGAAATGTGTTCCTGCGACAGGCTGAAATCGAGAACCCCCTGGAGGATCCTGTG
  ACTGGCGACTACGTGCACAAGTCTGTGTTTATCATTTTCTTCCAAGGCGATCAGCTGAAAAACAGAGTCA
  AGAAAATCTGTGAAGGGTTCCGAGCCTCACTCTATCCCTGTCCTGAGACACCACAGGAGAGGAAGGAAAT
  GGCTTCTGGAGTGAATACCAGGATTGATGATCTCCAAATGGTTCTGAATCAAACGGAGGATCACCGCCAG
  AGGGTTCTGCAGGCAGCTGCTAAGAACATCCGTGTCTGGTTCATCAAAGTGCGGAAGATGAAGGCCATCT
  ATCACACCCTGAACCTGTGCAACATAGATGTGACTCAGAAATGCTTGATTGCAGAGGTCTGGTGCCCTGT
  CACCGACCTTGACTCCATCCAGTTTGCACTCAGAAGGGGCACGGAACACAGTGGTTCCACTGTACCTTCC
  ATTTTGAACAGGATGCAGACAAACCAGACTCCCCCAACCTATAACAAAACCAACAAGTTTACCTATGGCT
  TTCAGAACATAGTAGATGCTTATGGAATTGGAACTTACCGAGAGATAAATCCAGCTCCGTATACTATTAT
  CACGTTCCCTTTTCTATTTGCTGTGATGTTTGGAGACTTCGGTCATGGCATTTTAATGACCCTTTTTGCT
  GTGTGGATGGTACTGAGGGAGAGCCGGATCCTTTCCCAGAAGAATGAGAATGAGATGTTTAGCACTGTGT
  TCAGTGGTCGATACATTATTTTATTGATGGGTGTGTTCTCCATGTACACTGGCCTCATCTACAATGATTG
  CTTTTCCAAGTCTCTTAATATCTTTGGGTCATCCTGGAGTGTACGGCCGATGTTTACTTATAATTGGACT
  GAAGAGACGCTTCGGGGGAACCCTGTTCTACAGCTGAACCCAGCCCTCCCTGGAGTGTTTGGTGGACCAT
  ACCCTTTTGGCATTGATCCAATTTGGAACATTGCTACCAATAAACTGACGTTCTTGAACTCCTTTAAGAT
  GAAGATGTCTGTTATCCTTGGTATCATCCATATGCTGTTTGGAGTCAGCCTGAGTCTGTTCAACCATATC
  TATTTCAAGAAGCCCCTGAATATCTACTTTGGATTTATTCCTGAAATAATCTTCATGACCTCTTTGTTTG
  GCTATTTGGTTATCCTTATTTTTTACAAGTGGACGGCCTATGATGCTCATACCTCTGAGAATGCACCAAG
  CCTTCTGATCCATTTCATAAACATGTTCCTCTTTTCCTACCCAGAGTCTGGTTATTCAATGTTGTATTCT
  GGACAGAAAGGAATTCAGTGTTTCCTGGTAGTGGTTGCACTACTGTGTGTACCTTGGATGCTGCTGTTTA
  AACCATTGGTCCTTCGCCGTCAGTATTTGAGGAGAAAGCATTTGGGAACTCTCAACTTTGGTGGGATCAG
  GGTGGGCAACGGACCGACAGAGGAGGATGCTGAGATTATTCAGCATGACCAGCTCTCCACCCACTCAGAG
  GACGCAGACGAGCCTTCCGAGGACGAAGTGTTTGACTTTGGGGACACCATGGTCCACCAGGCCATCCACA
  CCATCGAGTACTGCCTGGGCTGCATCTCCAACACTGCCTCCTACTTGCGGCTCTGGGCCCTCAGCCTCGC
  TCATGCGCAGCTGTCTGAGGTGCTTTGGACCATGGTGATCCACATCGGCCTGAGCGTGAAGAGCTTGGCG
  GGAGGTTTGGTGCTGTTCTTCTTCTTCACTGCCTTTGCCACCCTGACCGTGGCCATCCTCCTGATCATGG
  AGGGCCTCTCGGCCTTTCTCCACGCACTGCGCTTACACTGGGTTGAGTTCCAGAATAAATTCTACAGCGG
  GACCGGTTTCAAGTTCTTACCCTTCTCCTTCGAGCATATTCGGGAAGGGAAGTTTGAAGAGTGA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_001130021
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130021.1, NP_001123493.1
• RefSeq Size: 4205 bp
• RefSeq ORF: 2514 bp
• Locus ID: 535
• UniProt ID: Q93050
• Cytogenetics: 17q21.2
• Protein Families: Transmembrane
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction and contains an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.