LC3B (MAP1LC3B) (NM_022818) Human Untagged Clone

CAT#: SC322139

MAP1LC3B (untagged)-Human microtubule-associated protein 1 light chain 3 beta (MAP1LC3B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LC3B
• Synonyms: ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322139 ACGGGCGAGCGGCGACCCTGCCTCCCGGAGACGGCGCGGCCTGCCCTGCGCGCCTCAGCC
  CCGGGTGCCGGCGTCTCGGGCAGCACCACCAAGTCTCTCTGGAGGGGAAAGGATGGTCGG
  ATTTGCCCCATGTCCCTTTCTCTGACCCCTCCCTCAAGAGTGCCCCGGGACACCCCGCCT
  GTGGCTCAGCCTCCCCCGCCCCGCGCTGCCATCTCCTCAGGGCCGGGCAGCAGGCTCCCG
  AGCGCCCACAGACCCGGGGTGCGGCCCAGCCCACAACCGTCACCTCAGGGGCCTCAGGCG
  CCCAGCGGTGCTGGGCGGGGCTGGGGCACGACCGGGAGCATGCGCAGAGCGCGCGTTTCG
  CCCATCGCGCACGCGCACACACCTGCTCCGCCCCCACGCTGCGTGCCGCTGCTGGGTTCC
  GCCACGCCCGTCATGGCGGCGGCCCCGGCCGGCTCTGGCCCCGCCCCTCGGTGACGCGTC
  GCGAGTCACCTGACCAGGCTGCGGGCTGAGGAGATACAAGGGAAGTGGCTATCGCCAGAG
  TCGGATTCGCCGCCGCAGCAGCCGCCGCCCCCGGGAGCCGCCGGGACCCTCGCGTCGTCG
  CCGCCGCCGCCGCCCAGATCCCCGCACCATGCCGTCGGAGAAGACCTTCAAGCAGCGCCG
  CACCTTCGAACAAAGAGTAGAAGATGTCCGACTTATTCGAGAGCAGCATCCAACCAAAAT
  CCCGGTGATAATAGAACGATACAAGGGTGAGAAGCAGCTTCCTGTTCTGGATAAAACAAA
  GTTCCTTGTACCTGACCATGTCAACATGAGTGAGCTCATCAAGATAATTAGAAGGCGCTT
  ACAGCTCAATGCTAATCAGGCCTTCTTCCTGTTGGTGAACGGACACAGCATGGTCAGCGT
  CTCCACACCAATCTCAGAGGTGTATGAGAGTGAGAAAGATGAAGATGGATTCCTGTACAT
  GGTCTATGCCTCCCAGGAGACGTTCGGGATGAAATTGTCAGTGTAAAACCAGAAAAAATG
  CATCTCTTCTAGAATTGTTTAAACCCTTACCAAGGAAAAAAAAGGGGTGTTACCAACTGA
  GATCGATCAGTTCATCCAATCACAGATCATGAAACAGTAGTGTTCCCACCTAGGAGTGTT
  AGGAAGTTGTGTTTGTGTTTCAAGCAGAAAAACTGAGCTCCAAGTGAGCACATTCAGCTT
  TGGAAACTATATTATTTAATGTAGGCTAGCTTGTTTTCAAATTTTAAAAGTTTAAAAATA
  AAATACTTTGCATTCTAAGTTGCCAATAAAATAGACCTTCAAGTTATTTTAATGCTCTTT
  TCTCACTAATAGGAACTTGTAATTCCAGCAGTAATTTAAAGGCTTTCAGAGAGACCCTGA
  GTCTTCTCTTCAGGTTCACAGAACCCGCCGCCTTTTTGGGTAGAAGTTTTCTACTCAGCT
  AGAGAGATCTCCCTAAGAGGATCTTTAGGCCTGAGTTGTGAAGCGCAACCCCCGCAAAAC
  GCATTTGCCATCACAGTTGGCACAAACGCAGGGTAAACGGGCTGTGTGAGAAAACGGCCC
  TGACTGTAAACTGCTGAAGGTCCCTGACTCCTAAGAGAACCACACCCAAAGTCCTCACTC
  TTGCAGGGGTAGACATTTCTGGTTTGGTTTGTTCTCTAGATAGTTACACACATAAAGACA
  CCACTCAAAAGGAAACTTGAATAATTTATAATTTTGATCGAGTTTCTTAAAAGACCCTGG
  AGAAAGAGTGGCATTTCTTCTGTTTCAGGTTTTGTCTGAGTTCAAACTAGTGCCTGTGTT
  GTTACGGAAAGCAGCAGTGTACCAGTGTCACTCTGGAGTACAGCGGGAGAAACACAAAAT
  AGTATAACTGAAAACATTAACATTCAGACACACTCCCTTCTGCCTTCCGGCTTAAAGCTG
  TGGATGATCCACGTTTTTGTTTTTTTAATGTTAAATGTGTAACTCAGTATTACTGAAAAG
  GTACCCACATTTTGAATAGTAGTTATCACTCTTAGGTCAGACAGCCATCAGAATTCTCCC
  ACACCAAGTGCATGTCAGTTGTGGAGAAAACATAGCAAAAAGAGCCGTACGCTCTTTACA
  GATACTAATGTCAAGAGTTAAACCTCCTCAGGTTCAACCTGTGATAAAAGACTAGTGCTT
  CCCAGTACTTGCATGGGGTTCACTATTTATAGTTTTCTTGGGAGTATCACAGGAAAATCA
  CAATTACACCACTTTAGACCCTATGTGTAGCAGGTCACAACTTACCCTTGTGTGTTTAGA
  TGTGTATGAAATACCTGTATACGTTAGTGAAAGCTGTTTACTGTAACGGGGAAAACCAGA
  TTCTTTGCATCTGGGCCCTCTACTGATTGTTAAAGGAGTTCCTGTCACCTGCTCCCCCCA
  CCCCCGCATGCGTCTGTCCACTTGGCTAACTTTTAATATGTGTATTTTTACATTATGTAT
  ATTCTTAACTGGACTGTCTCGTTTAGACTGTATACATCATATCTGACATTATTGTAACTA
  CCGTGTGATCAGTAAGATTCCTGTAAGAAATACTGCTTTTTAAGAAAAAAAATAACATGC
  TGAGGGGTGACCTATATCCCATGTGAGTGGTCACTTTATTTATAGGATCTTTAAAACATT
  TTTAATGAACTAAGTTGAATAAAGGCACAATTAAAAACTGTCATCAAAAAAAAAAAAAAA
  A
• Restriction Sites: Please inquire
• ACCN: NM_022818
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022818.3, NP_073729.1
• RefSeq Size: 2270 bp
• RefSeq ORF: 378 bp
• Locus ID: 81631
• UniProt ID: Q9GZQ8
• Cytogenetics: 16q24.2
• Domains: MAP1_LC3
• Gene Summary: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]