DDX47 (NM_016355) Human Untagged Clone

CAT#: SC322007

DDX47 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 47 (DDX47), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX47
• Synonyms: E4-DBP; HQ0256; MSTP162; RRP3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322007 CAAGATGGCGGCACCCGAGGAACACGATTCTCCGACCGAAGCGTCCCAGCCGATTGTGGA
  AGAGGAGGAAACTAAAACATTTAAAGACCTGGGTGTGACAGATGTGTTGTGTGAAGCTTG
  TGACCAGTTGGGATGGACAAAACCCACCAAGATCCAGATTGAAGCTATTCCTTTGGCCTT
  ACAAGGTCGTGATATCATTGGGCTTGCAGAAACTGGCTCTGGAAAGACAGGCGCCTTTGC
  TTTGCCCATTCTAAACGCACTGCTGGAGACCCCGCAGCGTTTGTTTGCCCTAGTTCTTAC
  CCCGACTCGGGAGCTGGCCTTTCAGATCTCAGAGCAGTTTGAAGCCCTGGGGTCCTCTAT
  TGGAGTGCAGAGTGCTGTGATTGTAGGTGGAATTGATTCAATGTCTCAATCTTTGGCCCT
  TGCAAAAAAACCACATATAATAATAGCAACTCCTGGTCGACTGATTGACCACTTGGAAAA
  TACGAAAGGTTTCAACTTGAGAGCTCTCAAATACTTGGTCATGGATGAAGCCGACCGAAT
  ACTGAATATGGATTTTGAGACAGAGGTTGACAAGATCCTCAAAGTGATTCCTCGAGATCG
  GAAAACATTCCTCTTCTCTGCCACCATGACCAAGAAGGTTCAAAAACTTCAGCGAGCAGC
  TCTGAAGAATCCTGTGAAATGTGCCGTTTCCTCTAAATACCAGACAGTTGAAAAATTACA
  GCAATATTATATTTTTATTCCCTCTAAATTCAAGGATACCTACCTGGTTTATATTCTAAA
  TGAATTGGCTGGAAACTCCTTTATGATATTCTGCAGCACCTGTAATAATACCCAGAGAAC
  AGCTTTGCTACTGCGAAATCTTGGCTTCACTGCCATCCCCCTCCATGGACAAATGAGTCA
  GAGTAAGCGCCTAGGATCCCTTAATAAGTTTAAGGCCAAGGCCCGTTCCATTCTTCTAGC
  AACTGACGTTGCCAGCCGAGGTTTGGACATACCTCATGTAGATGTGGTTGTCAACTTTGA
  CATTCCTACCCATTCCAAGGATTACATCCATCGAGTAGGTCGAACAGCTAGAGCTGGGCG
  CTCCGGAAAGGCTATTACTTTTGTCACACAGTATGATGTGGAACTCTTCCAGCGCATAGA
  ACACTTAATTGGGAAGAAACTACCAGGTTTTCCAACACAGGATGATGAGGTTATGATGCT
  GACAGAACGCGTCGCTGAAGCCCAAAGGTTTGCCCGAATGGAGTTAAGGGAGCATGGAGA
  AAAGAAGAAACGCTCGCGAGAGGATGCTGGAGATAATGATGACACAGAGGGTGCTATTGG
  TGTCAGGAACAAGGTGGCTGGAGGAAAAATGAAGAAGCGGAAAGGCCGTTAATCACTTTT
  ATGAAGGCTCGAGTTCTGCTGTTCTGTAAAAGAGAATTGGAGAATGAAACCTGCTCCAAC
  AGAGATCATGAGACTGAAATTGGTCAGAATTGTGTCCAGAATGTGCTCAGCTAATTCAGT
  ATTCTTCCCCATTCTGGGTTGGAGTTTACTGCAGAGTAATTCTTACAGTGCTGATGTCAA
  GACTGTTACTGTTCTTCGACTTTGATTCCTTGCTCATGACATGAGTAGGGTGTGCTCTTC
  TGTCACTTCACACAGACCTTTTGCCTTTTTTAGCTGCAAGTCAAGGACTAGGTTGATGAT
  GCCCATGACCTGTAATTGTAAAGAAGCTTGGACATCTGCAAATGATATTTAAACCATCTT
  GGCTTGTGCTTTATTCAAACTAATGTGAAACAATAAATTTAAATATTATTTTTAAAAGAA
  AAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_016355
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016355.3, NP_057439.2
• RefSeq Size: 1836 bp
• RefSeq ORF: 1368 bp
• Locus ID: 51202
• UniProt ID: Q9H0S4
• Cytogenetics: 12p13.1
• Domains: DEAD, helicase_C
• Gene Summary: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).