GLUD2 (NM_012084) Human Untagged Clone

CAT#: SC321890

GLUD2 (untagged)-Human glutamate dehydrogenase 2 (GLUD2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLUD2
• Synonyms: GDH2; GLUDP1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012084.3 TTCCTTCCTAGTCGCGGGGAGTCTGAGAAAGCGCACCTGTTCCGCGACCGTCACGCACCC
  CTCCTCCGCCTGCCGCGATGTACCGCTACCTGGCCAAAGCGCTGCTGCCGTCCCGGGCCG
  GGCCCGCTGCCCTGGGCTCCGCGGCCAACCACTCGGCCGCGTTGCTGGGCCGGGGCCGCG
  GACAGCCCGCCGCCGCCTCGCAGCCGGGGCTCGCATTGGCCGCCCGGCGCCACTACAGCG
  AGTTGGTGGCCGACCGCGAGGACGACCCCAACTTCTTCAAGATGGTGGAGGGCTTCTTCG
  ATCGCGGCGCCAGCATCGTGGAGGACAAGTTGGTGAAGGACCTGAGGACCCAGGAAAGCG
  AGGAGCAGAAGCGGAACCGGGTGCGCGGCATCCTGCGGATCATCAAGCCCTGCAACCATG
  TGCTGAGTCTCTCCTTCCCCATCCGGCGCGACGACGGCTCCTGGGAGGTCATCGAAGGCT
  ACCGGGCCCAGCACAGCCAGCACCGCACGCCCTGCAAGGGAGGTATCCGTTACAGCACTG
  ATGTGAGTGTAGATGAAGTAAAAGCTTTGGCTTCTCTGATGACATACAAGTGTGCAGTGG
  TTGATGTGCCGTTTGGGGGTGCTAAAGCTGGTGTTAAGATCAATCCCAAGAACTATACCG
  AAAATGAATTGGAAAAGATCACAAGGAGGTTCACCATGGAGCTAGCAAAGAAGGGCTTTA
  TTGGTCCTGGCGTTGATGTGCCTGCTCCAGACATGAACACAGGTGAGCGGGAGATGTCCT
  GGATTGCTGATACCTATGCCAGCACCATAGGGCACTATGATATTAATGCACACGCCTGTG
  TTACTGGTAAACCCATCAGCCAAGGGGGAATCCATGGACGCATCTCTGCTACTGGCCGTG
  GTGTCTTCCATGGGATTGAAAACTTCATCAATGAAGCTTCTTACATGAGCATTTTAGGAA
  TGACACCAGGGTTTAGAGATAAAACATTTGTTGTTCAGGGATTTGGTAATGTGGGCCTAC
  ACTCTATGAGATATTTACATCGTTTTGGTGCTAAATGTATTGCTGTTGGTGAGTCTGATG
  GGAGTATATGGAATCCAGATGGTATTGACCCAAAGGAACTGGAAGACTTCAAATTGCAAC
  ATGGGTCCATTCTGGGCTTCCCCAAGGCAAAGCCCTATGAAGGAAGCATCTTGGAGGTCG
  ACTGTGACATACTGATCCCAGCTGCCACTGAGAAGCAGTTGACCAAATCCAACGCACCCA
  GAGTCAAAGCCAAGATCATTGCTGAAGGTGCCAATGGGCCAACAACTCCAGAAGCTGATA
  AGATCTTCCTGGAGAGAAACATTTTGGTTATTCCAGATCTCTACTTGAATGCTGGAGGAG
  TGACAGTATCTTACTTTGAGTGGCTGAAGAATCTAAATCATGTCAGCTATGGCCGTTTGA
  CCTTCAAATATGAAAGGGATTCTAACTACCACTTGCTCCTGTCTGTTCAAGAGAGTTTAG
  AAAGAAAATTTGGAAAGCATGGTGGAACTATTCCCATTGTACCCACGGCAGAGTTCCAAG
  ACAGTATATCGGGTGCATCTGAGAAAGACATTGTGCACTCTGCCTTGGCATACACAATGG
  AGCGTTCTGCCAGGCAAATTATGCACACAGCCATGAAGTATAACCTGGGATTGGACCTGA
  GAACAGCTGCCTATGTCAATGCCATTGAAAAAGTCTTCAAAGTGTACAGTGAAGCTGGTG
  TGACCTTCACATAGATGGATCATGGCTGACTTCCTCACTAACCTCTTCACGTGTAACTTC
  TGCAGACCTACCACAAGTTTACATGTAACCACAGAAATCCCTTTCTCTCCTGACTCATTA
  CTAATGGATACCATTCTCAACAAGTCAATCCAAATCAGCCCGTTAAGGAGAAAGAAATTA
  ATATACAAGCTGAGTGTGAAAGTAGAAATCACCTACACCAGAGAGCTATTTTGGTATTTT
  GCCTTTAAATAAAAAGCCTCCTCCATATGGCTGTGCAGCCTTGCTCTGTGGCTTTTCCCA
  GCACAATCAGTGCTAGTGCTGGGGAAGGGACAGTCAAGAGCAGTCAGTTGCTTACTTATT
  TTGCTCTGGATGAGTCTGGGACACGCTGTAACTTTAACACATTTAAGAAGAAGGTGTGTG
  GCCTTTTCAGAAGGTGGCATGGTCCTCAAGTGAGTTCTTAGTATTTTATATCAGCAAAAT
  AACTCAATTTTGCAGATTGCAAACAAATATAAAAGCTGTTTCTGTTTATGAATTTTATTC
  TTTTAGAATAGAATAAGTACATGCTGCTGTAATAAAATTGCCTTTAATCACTTAAAAAAA
  AAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_012084
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012084.3, NP_036216.2
• RefSeq Size: 2348 bp
• RefSeq ORF: 1677 bp
• Locus ID: 2747
• UniProt ID: P49448
• Cytogenetics: Xq24
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism
• Gene Summary: The protein encoded by this gene is localized to the mitochondrion and acts as a homohexamer to recycle glutamate during neurotransmission. The encoded enzyme catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate. This gene is intronless.[provided by RefSeq, Jan 2010]