DARC (ACKR1) (NM_002036) Human Untagged Clone

CAT#: SC321096

DARC (untagged)-Human Duffy blood group, chemokine receptor (DARC), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DARC
• Synonyms: CCBP1; CD234; DARC; DARC/ACKR1; Dfy; FY; GPD; GpFy; WBCQ1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002036.2 GCAGGCAGTGGGCGTGGGGTAAGGCTTCCTGATGCCCCCTGTCCCTGCCCAGAACCTGAT
  GGCCCTCATTAGTCCTTGGCTCTTATCTTGGAAGCACAGGCGCTGACAGCCGTCCCAGCC
  CTTCTGTCTGCGGGCCTGAACCAAACGGTGCCATGGGGAACTGTCTGCACAGGGCGGAGC
  TCTCCCCCTCAACTGAGAACTCAAGTCAGCTGGACTTCGAAGATGTATGGAATTCTTCCT
  ATGGTGTGAATGATTCCTTCCCAGATGGAGACTATGGTGCCAACCTGGAAGCAGCTGCCC
  CCTGCCACTCCTGTAACCTGCTGGATGACTCTGCACTGCCCTTCTTCATCCTCACCAGTG
  TCCTGGGTATCCTAGCTAGCAGCACTGTCCTCTTCATGCTTTTCAGACCTCTCTTCCGCT
  GGCAGCTCTGCCCTGGCTGGCCTGTCCTGGCACAGCTGGCTGTGGGCAGTGCCCTCTTCA
  GCATTGTGGTGCCCGTCTTGGCCCCAGGGCTAGGTAGCACTCGCAGCTCTGCCCTGTGTA
  GCCTGGGCTACTGTGTCTGGTATGGCTCAGCCTTTGCCCAGGCTTTGCTGCTAGGGTGCC
  ATGCCTCCCTGGGCCACAGACTGGGTGCAGGCCAGGTCCCAGGCCTCACCCTGGGGCTCA
  CTGTGGGAATTTGGGGAGTGGCTGCCCTACTGACACTGCCTGTCACCCTGGCCAGTGGTG
  CTTCTGGTGGACTCTGCACCCTGATATACAGCACGGAGCTGAAGGCTTTGCAGGCCACAC
  ACACTGTAGCCTGTCTTGCCATCTTTGTCTTGTTGCCATTGGGTTTGTTTGGAGCCAAGG
  GGCTGAAGAAGGCATTGGGTATGGGGCCAGGCCCCTGGATGAACATCCTGTGGGCCTGGT
  TTATTTTCTGGTGGCCTCATGGGGTGGTTCTAGGACTGGATTTCCTGGTGAGGTCCAAGC
  TGTTGCTGTTGTCAACATGTCTGGCCCAGCAGGCTCTGGACCTGCTGCTGAACCTGGCAG
  AAGCCCTGGCAATTTTGCACTGTGTGGCTACGCCCCTGCTCCTCGCCCTATTCTGCCACC
  AGGCCACCCGCACCCTCTTGCCCTCTCTGCCCCTCCCTGAAGGATGGTTTTCTCATCTGG
  ACACCCTTGGAAGCAAATCCTAGTTCTCTTCCCACCTGTCAACCTGAATTAAAGTCTACA
  CTGCCTTTGTGATAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002036
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002036.2, NP_002027.2
• RefSeq Size: 1317 bp
• RefSeq ORF: 1011 bp
• Locus ID: 2532
• UniProt ID: Q16570
• Cytogenetics: 1q23.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: The protein encoded by this gene is a glycosylated membrane protein and a non-specific receptor for several chemokines. The encoded protein is the receptor for the human malarial parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a.