Aldolase (ALDOA) (NM_000034) Human Untagged Clone

CAT#: SC320738

ALDOA (untagged)-Human aldolase A, fructose-bisphosphate (ALDOA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Aldolase
• Synonyms: ALDA; GSD12; HEL-S-87p
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000034.2 CCTAGCTTGGCGCGGAATCCGTGAATTGCCCGCGGCCCGAGGGTGCAGCTCCCGGACTGA
  CTGGCTCTGCCCTTCCCCATGGACGCCTCCTCTAGCCCGTGGAATCCAACCCCGGCTCCT
  GTCAGCAGCCCTCCCCTGCTGCTCCCCATCCCTGCCATCGTCTTCATCGCTGTGGGCATC
  TATTTGTTGCTGCTGGGTCTAGTCCTGCTGACTAGGAACTGCCTGCTGGCCCAGGGCTGC
  TGCGCGGACGGTAGCTCCCCCTGCAGGAAGCAAGGTTCCTCCGGGCCCCCAGACTGCTGC
  TGGACCTGTGCAGAAGCCTGCAACTTTCCTCTGCCTAGCCCGGCCCACTTCCTGGATGCT
  TGCTGCCCCCAGCCCACCAGAGCTGACTGGGCACCTCGCTGCCCCCGCTGCTGCCCACTC
  TGCGACTGTGCCTGTACGTGCCAGCTCCCCGACTGCCAGAGCCTCAACTGTCTCTGCTTC
  GAGATCAAGCTCCGATGAGGACCCAGGGCCCCTGCCCTCTGGGGAGCGGCCAGCCCCCAG
  GGCCCATGTGCCCTCCTCCCTGAAGAGCCTTTCCCCACGCCACTGGAACCACAGATGGCC
  TGCCGAGCACCCAGGCCTGGGAACTGGAAGTGGCAGCGCAGGGCCTGGCTCCCTGCAGGG
  CAGGACTCTTGGCCGGCTGGACGGCAGCTCCTCTGGAGGGCCAGAAAAGAGAGGGGCTAG
  TGCTCGGGCAGGTGCCCTGGCTTCCCTTCCCCTCCACACGTCAACGATTCTATTTGAAGT
  TGGGCAGGGGGGTGGCGCTGCTCACCACACACAAGTGTTATAGGAGGAGTCTGGCCCTTG
  AGTACCGGGTACGCAGGGGTGCCTCAACCACACTCCGTCCACGGACTCTCCGTTATTTTA
  GGAGGTCCCTGGCCAAAGATTTATTTCTCTTGACAACCAAGGGCCTCCGTCTGGATTTCC
  AAGGAAGAATTTCCTCTGAAGCACCGGAACTTGCTACTACCAGCACCATGCCCTACCAAT
  ATCCAGCACTGACCCCGGAGCAGAAGAAGGAGCTGTCTGACATCGCTCACCGCATCGTGG
  CACCTGGCAAGGGCATCCTGGCTGCAGATGAGTCCACTGGGAGCATTGCCAAGCGGCTGC
  AGTCCATTGGCACCGAGAACACCGAGGAGAACCGGCGCTTCTACCGCCAGCTGCTGCTGA
  CAGCTGACGACCGCGTGAACCCCTGCATTGGGGGTGTCATCCTCTTCCATGAGACACTCT
  ACCAGAAGGCGGATGATGGGCGTCCCTTCCCCCAAGTTATCAAATCCAAGGGCGGTGTTG
  TGGGCATCAAGGTAGACAAGGGCGTGGTCCCCCTGGCAGGGACAAATGGCGAGACTACCA
  CCCAAGGGTTGGATGGGCTGTCTGAGCGCTGTGCCCAGTACAAGAAGGACGGAGCTGACT
  TCGCCAAGTGGCGTTGTGTGCTGAAGATTGGGGAACACACCCCCTCAGCCCTCGCCATCA
  TGGAAAATGCCAATGTTCTGGCCCGTTATGCCAGTATCTGCCAGCAGAATGGCATTGTGC
  CCATCGTGGAGCCTGAGATCCTCCCTGATGGGGACCATGACTTGAAGCGCTGCCAGTATG
  TGACCGAGAAGGTGCTGGCTGCTGTCTACAAGGCTCTGAGTGACCACCACATCTACCTGG
  AAGGCACCTTGCTGAAGCCCAACATGGTCACCCCAGGCCATGCTTGCACTCAGAAGTTTT
  CTCATGAGGAGATTGCCATGGCGACCGTCACAGCGCTGCGCCGCACAGTGCCCCCCGCTG
  TCACTGGGATCACCTTCCTGTCTGGAGGCCAGAGTGAGGAGGAGGCGTCCATCAACCTCA
  ATGCCATTAACAAGTGCCCCCTGCTGAAGCCCTGGGCCCTGACCTTCTCCTACGGCCGAG
  CCCTGCAGGCCTCTGCCCTGAAGGCCTGGGGCGGGAAGAAGGAGAACCTGAAGGCTGCGC
  AGGAGGAGTATGTCAAGCGAGCCCTGGCCAACAGCCTTGCCTGTCAAGGAAAGTACACTC
  CGAGCGGTCAGGCTGGGGCTGCTGCCAGCGAGTCCCTCTTCGTCTCTAACCACGCCTATT
  AAGCGGAGGTGTTCCCAGGCTGCCCCCAACACTCCAGGCCCTGCCCCCTCCCACTCTTGA
  AGAGGAGGCCGCCTCCTCGGGGCTCCAGGCTGGCTTGCCCGCGCTCTTTCTTCCCTCGTG
  ACAGTGGTGTGTGGTGTCGTCTGTGAATGCTAAGTCCATCACCCTTTCCGGCACACTGCC
  AAATAAACAGCTATTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAACTCGGG
• Restriction Sites: Please inquire
• ACCN: NM_000034
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000034.2, NP_000025.1
• RefSeq Size: 2353 bp
• RefSeq ORF: 1095 bp
• Locus ID: 226
• UniProt ID: P04075
• Cytogenetics: 16p11.2
• Domains: glycolytic_enzy
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway
• Gene Summary: This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Sep 2017]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 2, 3, 4, 7, 8, 9, and 10 encode the same isoform.