CDC20 (NM_001255) Human Untagged Clone

CAT#: SC320629

CDC20 (untagged)-Human cell division cycle 20 homolog (S. cerevisiae) (CDC20)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC20
• Synonyms: bA276H19.3; CDC20A; p55CDC
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001255.2 GAGGCGTAAGCCAGGCGTGTTAAAGCCGGTCGGAACTGCTCCGGAGGGCACGGGCTCCGT
  AGGCACCAACTGCAAGGACCCCTCCCCCTGCGGGCGCTCCCATGGCACAGTTCGCGTTCG
  AGAGTGACCTGCACTCGCTGCTTCAGCTGGATGCACCCATCCCCAATGCACCCCCTGCGC
  GCTGGCAGCGCAAAGCCAAGGAAGCCGCAGGCCCGGCCCCCTCACCCATGCGGGCCGCCA
  ACCGATCCCACAGCGCCGGCAGGACTCCGGGCCGAACTCCTGGCAAATCCAGTTCCAAGG
  TTCAGACCACTCCTAGCAAACCTGGCGGTGACCGCTATATCCCCCATCGCAGTGCTGCCC
  AGATGGAGGTGGCCAGCTTCCTCCTGAGCAAGGAGAACCAGCCTGAAAACAGCCAGACGC
  CCACCAAGAAGGAACATCAGAAAGCCTGGGCTTTGAACCTGAACGGTTTTGATGTAGAGG
  AAGCCAAGATCCTTCGGCTCAGTGGAAAACCACAAAATGCGCCAGAGGGTTATCAGAACA
  GACTGAAAGTACTCTACAGCCAAAAGGCCACTCCTGGCTCCAGCCGGAAGACCTGCCGTT
  ACATTCCTTCCCTGCCAGACCGTATCCTGGATGCGCCTGAAATCCGAAATGACTATTACC
  TGAACCTTGTGGATTGGAGTTCTGGGAATGTACTGGCCGTGGCACTGGACAACAGTGTGT
  ACCTGTGGAGTGCAAGCTCTGGTGACATCCTGCAGCTTTTGCAAATGGAGCAGCCTGGGG
  AATATATATCCTCTGTGGCCTGGATCAAAGAGGGCAACTACTTGGCTGTGGGCACCAGCA
  GTGCTGAGGTGCAGCTATGGGATGTGCAGCAGCAGAAACGGCTTCGAAATATGACCAGTC
  ACTCTGCCCGAGTGGGCTCCCTAAGCTGGAACAGCTATATCCTGTCCAGTGGTTCACGTT
  CTGGCCACATCCACCACCATGATGTTCGGGTAGCAGAACACCATGTGGCCACACTGAGTG
  GCCACAGCCAGGAAGTGTGTGGGCTGCGCTGGGCCCCAGATGGACGACATTTGGCCAGTG
  GTGGTAATGATAACTTGGTCAATGTGTGGCCTAGTGCTCCTGGAGAGGGTGGCTGGGTTC
  CTCTGCAGACATTCACCCAGCATCAAGGGGCTGTCAAGGCCGTAGCATGGTGTCCCTGGC
  AGTCCAATGTCCTGGCAACAGGAGGGGGCACCAGTGATCGACACATTCGCATCTGGAATG
  TGTGCTCTGGGGCCTGTCTGAGTGCCGTGGATGCCCATTCCCAGGTGTGCTCCATCCTCT
  GGTCTCCCCATTACAAGGAGCTCATCTCAGGCCATGGCTTTGCACAGAACCAGCTAGTTA
  TTTGGAAGTACCCAACCATGGCCAAGGTGGCTGAACTCAAAGGTCACACATCCCGGGTCC
  TGAGTCTGACCATGAGCCCAGATGGGGCCACAGTGGCATCCGCAGCAGCAGATGAGACCC
  TGAGGCTATGGCGCTGTTTTGAGTTGGACCCTGCGCGGCGGCGGGAGCGGGAGAAGGCCA
  GTGCAGCCAAAAGCAGCCTCATCCACCAAGGCATCCGCTGAAGACCAACCCATCACCTCA
  GTTGTTTTTTATTTTTCTAATAAAGTCATGTCTCCCTTCATGTTTTTTTTTTAAAAAAAA
  AAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001255
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001255.2, NP_001246.2
• RefSeq Size: 1697 bp
• RefSeq ORF: 1500 bp
• Locus ID: 991
• UniProt ID: Q12834
• Cytogenetics: 1p34.2
• Domains: WD40
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Ubiquitin mediated proteolysis
• Gene Summary: CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008]