Glutathione Reductase (GSR) (NM_000637) Human Untagged Clone

CAT#: SC320532

GSR (untagged)-Human glutathione reductase (GSR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Glutathione Reductase
• Synonyms: GR; GSRD; HEL-75; HEL-S-122m
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000637 edited
  GTGAGGCTGCGCTTGCCCGGGGCCCGCGCCCCCCTACCCCGGGGACCGCCCCCGGGCCGC
  CCGCCCCACTTGGCGCGCCACTTCCGCGTGCATGGCCCTGCTGCCCCGAGCCCTGAGCGC
  CGGCGCGGGACCGAGCTGGCGGCGGGCGGCGCGCGCCTTCCGAGGCTTCCTGCTGCTTCT
  GCCCGAGCCCGCGGCCCTCACGCGCGCCCTCTCCCGTGCCATGGCCTGCAGGCAGGAGCC
  GCAGCCGCAGGGCCCGCCGCCCGCTGCTGGCGCCGTGGCCTCCTATGACTACCTGGTGAT
  CGGGGGCGGCTCGGGCGGGCTGGCCAGCGCGCGCAGGGCGGCCGAGCTGGGTGCCAGGGC
  CGCCGTGGTGGAGAGCCACAAGCTGGGTGGCACTTGCGTGAATGTTGGATGTGTACCCAA
  AAAGGTAATGTGGAACACAGCTGTCCACTCTGAATTCATGCATGATCATGCTGATTATGG
  CTTTCCAAGTTGTGAGGGTAAATTCAATTGGCGTGTTATTAAGGAAAAGCGGGATGCCTA
  TGTGAGCCGCCTGAATGCCATCTATCAAAACAATCTCACCAAGTCCCATATAGAAATCAT
  CCGTGGCCATGCAGCCTTCACGAGTGATCCCAAGCCCACAATAGAGGTCAGTGGGAAAAA
  GTACACCGCCCCACACATCCTGATCGCCACAGGTGGTATGCCCTCCACCCCTCATGAGAG
  CCAGATCCCCGGTGCCAGCTTAGGAATAACCAGCGATGGATTTTTTCAGCTGGAAGAATT
  GCCCGGCCGCAGCGTCATTGTTGGTGCAGGTTACATTGCTGTGGAGATGGCAGGGATCCT
  GTCAGCCCTGGGTTCTAAGACATCACTGATGATACGGCATGATAAGGTACTTAGAAGTTT
  TGATTCAATGATCAGCACCAACTGCACGGAGGAGCTGGAGAACGCTGGCGTGGAGGTGCT
  GAAGTTCTCCCAGGTCAAGGAGGTTAAAAAGACTTTGTCGGGCTTGGAAGTCAGCATGGT
  TACTGCAGTTCCCGGTAGGCTACCAGTCATGACCATGATTCCAGATGTTGACTGCCTGCT
  CTGGGCCATTGGGCGGGTCCCGAATACCAAGGACCTGAGTTTAAACAAACTGGGGATTCA
  AACCGATGACAAGGGTCATATCATCGTAGACGAATTCCAGAATACCAACGTCAAAGGCAT
  CTATGCAGTTGGGGATGTATGTGGAAAAGCTCTTCTTACTCCAGTTGCAATAGCTGCTGG
  CCGAAAACTTGCCCATCGACTTTTTGAATATAAGGAAGATTCCAAATTAGATTATAACAA
  CATCCCAACTGTGGTCTTCAGCCACCCCCCTATTGGGACAGTGGGACTCACGGAAGATGA
  AGCCATTCATAAATATGGAATAGAAAATGTGAAGACCTATTCAACGAGCTTTACCCCGAT
  GTATCACGCAGTTACCAAAAGGAAAACAAAATGTGTGATGAAAATGGTCTGTGCTAACAA
  GGAAGAAAAGGTGGTTGGGATCCATATGCAGGGACTTGGGTGTGATGAAATGCTGCAGGG
  TTTTGCTGTTGCAGTGAAGATGGGAGCAACGAAGGCAGACTTTGACAACACAGTCGCCAT
  TCACCCTACCTCTTCAGAAGAGCTGGTCACACTTCGTTGAGAACCAGGAGACACGTGTGG
  CGGGCAGTGGGACCCATAGATCTTCTGAAATGAAACAAATAATCACATTGAAAAAAAAAA
  AAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000637
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000637.2, NP_000628.2
• RefSeq Size: 2262 bp
• RefSeq ORF: 1569 bp
• Locus ID: 2936
• UniProt ID: P00390
• Cytogenetics: 8p12
• Domains: pyr_redox, pyr_redox_dim
• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism
• Gene Summary: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.