SEC11A (NM_014300) Human Untagged Clone

CAT#: SC320451

SEC11A (untagged)-Human SEC11 homolog A (S. cerevisiae) (SEC11A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEC11A
• Synonyms: 1810012E07Rik; SEC11L1; sid2895; SPC18; SPCS4A
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014300.2 GAAGGTGGCAGAGCAGCCTCCCCAACTAAGCCTGGGTCGCCGCCTCGGTGAAGGGCCGCG
  GGGCCGAGCGCGGGAAGTGGCGTCCAGTCGGTTTTTGGCAGGCGAGAGCCAGAGCGTGAG
  AACCCTCCCACGGCGAGGCCAAGCCCTGAGACTGGACTAGCAAGGTCGTGAGTCTCTCTG
  TCAGCTCTCCGCAGCGAAGGGGCGCGGGGGGCGGGGACCGTGCCCGACCGTGCCAGCTCC
  CGCTCCGGAAGCGGAAGTGCAAGCACTGCGGGGTCCTGTCCAGTGTGAGCCCGACCCGAG
  CTCCAGTAGTTCCGCCCGCTGGTCATCGCGCCCTTTCCCCTGCCGGTGTCCTGCTCGCCG
  TCCCCGCCATGCTGTCTCTAGACTTTTTGGACGATGTGCGGCGGATGAACAAGCGGCAGC
  TCTATTATCAAGTCCTAAATTTTGGAATGATTGTCTCATCGGCACTAATGATCTGGAAGG
  GGTTAATGGTAATAACTGGAAGTGAAAGTCCGATTGTAGTGGTGCTCAGTGGCAGCATGG
  AACCTGCATTTCATAGAGGAGATCTTCTCTTTCTAACAAATCGAGTTGAAGATCCCATAC
  GAGTGGGAGAAATTGTTGTTTTTAGGATAGAAGGAAGAGAGATTCCTATAGTTCACCGAG
  TCTTGAAGATTCATGAAAAGCAAAATGGGCATATCAAGTTTTTGACCAAAGGAGATAATA
  ATGCGGTTGATGACCGAGGCCTCTATAAACAAGGACAACATTGGCTAGAGAAAAAAGATG
  TTGTGGGGAGAGCCAGGGGATTTGTTCCTTATATTGGAATTGTGACGATCCTCATGAATG
  ACTATCCTAAATTTAAGTATGCAGTTCTCTTTTTGCTGGGTTTATTCGTGCTGGTTCATC
  GTGAGTAAGAAGCCTGCCTTGCTGTTCCTGGGAAGATGCCATAGTTTTCGTTACTGGATG
  TTTGGAGTAGATACTGGTCTGTGATTGGTGGAATGGAGAACACACGTGTTGGTGCTTCTG
  GGTAGCACTGGTTTGCATTAGTTTATGTTTCCATGCCAGAGTTTGTGTGGGCGGGCGCAT
  GTGCACCACAGAGTGCACTCGAGGGGACTTTCAGTCACAGGATTTCATAATTGTCATTGT
  CACACTTTCAAATTTTTGTACATCAGTGAATTTTTTTATATTAAAAGGTTGAGCCAAAGC
  CCCCAGTGTTTGTATTTTGAAGCCAAGCTTCACTTCTAAAGTGCCTACAGAGACTTGTAA
  ATGAAAATGCAGCTCTGCACGAGTTTGAAACCGTCATACCTCCTTCTATTAGGAATGGCA
  TATACTGAGGTGGTCGTAAGTCTTAACTTCTAAAATTTTAAATAAAAGACTTTGCACATT
  GAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_014300
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014300.2, NP_055115.1
• RefSeq Size: 1399 bp
• RefSeq ORF: 540 bp
• Locus ID: 23478
• UniProt ID: P67812
• Cytogenetics: 15q25.2-q25.3
• Domains: Peptidase_S26
• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Protein export
• Gene Summary: This gene encodes a member of the peptidase S26B family. The encoded protein is an 18kDa subunit of the signal peptidase complex and has been linked to cell migration and invasion, gastric cancer and lymph node metastasis. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (2) uses an alternate in-frame exon in the central coding region, compared to variant 1. It encodes a longer protein (isoform 2) compared to isoform 1.