Cathepsin K (CTSK) (NM_000396) Human Untagged Clone

CAT#: SC319622

CTSK (untagged)-Human cathepsin K (CTSK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cathepsin K
• Synonyms: CTS02; CTSO; CTSO1; CTSO2; PKND; PYCD
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000396.2 GATCACTGGAGCTGACTTCCGCAATCCCGATGGAATAAATCTAGCACCCCTGATGGTGTG
  CCCACACTTTGCTGCCGAAACGAAGCCAGACAACAGATTTCCATCAGCAGGATGTGGGGG
  CTCAAGGTTCTGCTGCTACCTGTGGTGAGCTTTGCTCTGTACCCTGAGGAGATACTGGAC
  ACCCACTGGGAGCTATGGAAGAAGACCCACAGGAAGCAATATAACAACAAGGTGGATGAA
  ATCTCTCGGCGTTTAATTTGGGAAAAAAACCTGAAGTATATTTCCATCCATAACCTTGAG
  GCTTCTCTTGGTGTCCATACATATGAACTGGCTATGAACCACCTGGGGGACATGACCAGT
  GAAGAGGTGGTTCAGAAGATGACTGGACTCAAAGTACCCCTGTCTCATTCCCGCAGTAAT
  GACACCCTTTATATCCCAGAATGGGAAGGTAGAGCCCCAGACTCTGTCGACTATCGAAAG
  AAAGGATATGTTACTCCTGTCAAAAATCAGGGTCAGTGTGGTTCCTGTTGGGCTTTTAGC
  TCTGTGGGTGCCCTGGAGGGCCAACTCAAGAAGAAAACTGGCAAACTCTTAAATCTGAGT
  CCCCAGAACCTAGTGGATTGTGTGTCTGAGAATGATGGCTGTGGAGGGGGCTACATGACC
  AATGCCTTCCAATATGTGCAGAAGAACCGGGGTATTGACTCTGAAGATGCCTACCCATAT
  GTGGGACAGGAAGAGAGTTGTATGTACAACCCAACAGGCAAGGCAGCTAAATGCAGAGGG
  TACAGAGAGATCCCCGAGGGGAATGAGAAAGCCCTGAAGAGGGCAGTGGCCCGAGTGGGA
  CCTGTCTCTGTGGCCATTGATGCAAGCCTGACCTCCTTCCAGTTTTACAGCAAAGGTGTG
  TATTATGATGAAAGCTGCAATAGCGATAATCTGAACCATGCGGTTTTGGCAGTGGGATAT
  GGAATCCAGAAGGGAAACAAGCACTGGATAATTAAAAACAGCTGGGGAGAAAACTGGGGA
  AACAAAGGATATATCCTCATGGCTCGAAATAAGAACAACGCCTGTGGCATTGCCAACCTG
  GCCAGCTTCCCCAAGATGTGACTCCAGCCAGCCAAATCCATCCTGCTCTTCCATTTCTTC
  CACGATGGTGCAGTGTAACGATGCACTTTGGAAGGGAGTTGGTGTGCTATTTTTGAAGCA
  GATGTGGTGATACTGAGATTGTCTGTTCAGTTTCCCCATTTGTTTGTGCTTCAAATGATC
  CTTCCTACTTTGCTTCTCTCCACCCATGACCTTTTTCACTGTGGCCATCAGGACTTTCCC
  TGACAGCTGTGTACTCTTAGGCTAAGAGATGTGACTACAGCCTGCCCCTGACTGTGTTGT
  CCCAGGGCTGATGCTGTACAGGTACAGGCTGGAGATTTTCACATAGGTTAGATTCTCATT
  CACGGGACTAGTTAGCTTTAAGCACCCTAGAGGACTAGGGTAATCTGACTTCTCACTTCC
  TAAGTTCCCTTCTATATCCTCAAGGTAGAAATGTCTATGTTTTCTACTCCAATTCATAAA
  TCTATTCATAAGTCTTTGGTACAAGTTTACATGATAAAAAGAAATGTGATTTGTCTTCCC
  TTCTTTGCACTTTTGAAATAAAGTATTTATCTCCTGTCTACAGTTTAATAAATAAAAAAA
  AAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000396
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000396.2, NP_000387.1
• RefSeq Size: 1702 bp
• RefSeq ORF: 990 bp
• Locus ID: 1513
• UniProt ID: P43235
• Cytogenetics: 1q21.3
• Domains: Pept_C1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome
• Gene Summary: The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]