Zyxin (ZYX) (NM_001010972) Human Untagged Clone

CAT#: SC319514

ZYX (untagged)-Human zyxin (ZYX), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Zyxin
• Synonyms: ESP-2; HED-2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001010972.1 GTGTCCCTCCCCGCCCGGCTGGAGGCTGCTCCGGACCGGGACGCAGAGTCTGCGGACCCG
  GCGCCGAGGCGGCCACCCGAGACGCGGCGCGCACGCTCCGGCCTGCGCAGCCCGGCCCGG
  CCATGGCGGCCCCCCGCCCGTCTCCCGCGATCTCCGTTTCGGTCTCGGCTCCGGCTTTTT
  ACGCCCCGCAGAAGAAGTTCGGCCCTGTGGTGGCCCCAAAGCCCAAAGTGAATCCCTTCC
  GGCCCGGGGACAGCGAGCCTCCCCCGGCACCCGGGGCCCAGCGCGCACAGATGGGCCGGG
  TGGGCGAGATTCCCCCGCCGCCCCCGGAAGACTTTCCCCTGCCTCCACCTCCCCTTGCTG
  GGGATGGCGACGATGCAGAGGGTGCTCTGGGAGGTGCCTTCCCGCCGCCCCCTCCCCCGA
  TCGAGGAATCATTTCCCCCTGCGCCTCTGGAGGAGGAGATCTTCCCTTCCCCGCCGCCTC
  CTCCGGAGGAGGAGGGAGGGCCTGAGGCCCCCATACCGCCCCCACCACAGCCCAGGGAGA
  AGGTGAGCAGTATTGATTTGGAGATCGACTCTCTGTCCTCACTGCTGGATGACATGACCA
  AGAATGATCCTTTCAAAGCCCGGGTGTCATCTGGATATGTGCCCCCACCAGTGGCCACTC
  CATTCAGTTCCAAGTCCAGTACCAAGCCTGCAGCCGGGGGCACAGCACCCCTGCCTCCTT
  GGAAGTCCCCTTCCAGCTCCCAGCCTCTGCCCCAGGTTCCGGCTCCGGCTCAGAGCCAGA
  CACAGTTCCATGTTCAGCCCCAGCCCCAGCCCAAGCCTCAGGTCCAACTCCATGTCCAGT
  CCCAGACCCAGCCTGTGTCTTTGGCTAACACCCAGCCCCGAGGGCCCCCAGCCTCATCTC
  CGGCTCCAGCCCCTAAGTTTTCTCCAGTGACTCCTAAGTTTACTCCTGTGGCTTCCAAGT
  TCAGTCCTGGAGCCCCAGGTGGATCTGGGTCACAACCAAATCAAAAATTGGGGCACCCCG
  AAGCTCTTTCTGCTGGCACAGGCTCCCCTCAACCTCCCAGCTTCACCTATGCCCAGCAGA
  GGGAGAAGCCCCGAGTGCAGGAGAAGCAGCACCCCGTGCCCCCACCGGCTCAGAACCAAA
  ACCAGGTGCGCTCCCCTGGGGCCCCAGGGCCCCTGACTCTGAAGGAGGTGGAGGAGCTGG
  AGCAGCTGACCCAGCAGCTAATGCAGGACATGGAGCATCCTCAGAGGCAGAATGTGGCTG
  TCAACGAACTCTGCGGCCGATGCCATCAACCCCTGGCCCGGGCGCAGCCAGCCGTCCGCG
  CTCTAGGGCAGCTGTTCCACATCGCCTGCTTCACCTGCCACCAGTGTGCGCAGCAGCTCC
  AGGGCCAGCAGTTCTACAGTCTGGAGGGGGCGCCGTACTGCGAGGGCTGTTACACTGACA
  CCCTGGAGAAGTGTAACACCTGCGGGGAGCCCATCACTGACCGCATGCTGAGGGCCACGG
  GCAAGGCCTATCACCCGCACTGCTTCACCTGTGTGGTCTGCGCCCGCCCCCTGGAGGGCA
  CCTCCTTCATCGTGGACCAGGCCAACCGGCCCCACTGTGTCCCCGACTACCACAAGCAGT
  ACGCCCCGAGGTGCTCCGTCTGCTCTGAGCCCATCATGCCTGAGCCTGGCCGAGATGAGA
  CTGTGCGAGTGGTCGCCCTGGACAAGAACTTCCACATGAAGTGTTACAAGTGTGAGGACT
  GCGGGAAGCCCCTGTCGATTGAGGCAGATGACAATGGCTGCTTCCCCCTGGACGGTCACG
  TGCTCTGTCGGAAGTGCCACACTGCTAGAGCCCAGACCTGAGTGAGGACAGGCCCTCTTC
  AGACCGCAGTCCATGCCCCATTGTGGACCACCCACACTGAGACCACCTGCCCCCACCTCA
  GTTATTGTTTTGATGTCTAGCCCCTCCCATTTCCAACCCCTCCCTAGCATCCCAGGTGCC
  CTGACCCAGGACCCAACATGGTCTAGGGATGCAGGATCCCCGCCCTGGGGTCTGGTCCTC
  GCCCATCCTGCAGGGATTGCCCACCGTCTTCCAGACACCCCACCTGAGGGGGGCACCAGG
  TTTAGTGCTGCTGCTTTCACTGCTGCACCCGCGCCCTCGGCCGGCCCCCCGAGCAGCCTT
  TGTACTCTGCTTGCGGAGGGCTGGGAGACCCTCCAGGACATTCCCACCCTCCCCCATGCT
  GCCAAGTTGTAGCTATAGCTACAAATAAAAAAAAACCTTGTTTTCCAGAAAAAAAAAAAA
  AAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001010972
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010972.1, NP_001010972.1
• RefSeq Size: 2322 bp
• RefSeq ORF: 1719 bp
• Locus ID: 7791
• UniProt ID: Q15942
• Cytogenetics: 7q34
• Protein Pathways: Focal adhesion
• Gene Summary: Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform.