Zyxin (ZYX) (NM_001010972) Human Tagged ORF Clone

CAT#: RC200960

ZYX (Myc-DDK-tagged)-Human zyxin (ZYX), transcript variant 2

Product Images

Circular map for RC200960

• 

  Coomassie blue staining of purified ZYX protein (Cat# TP300960). The protein was produced from HEK293T cells transfected with ZYX cDNA clone (Cat# RC200960) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zyxin
• Synonyms: ESP-2; HED-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200960 representing NM_001010972
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCCCCGCCCGTCTCCCGCGATCTCCGTTTCGGTCTCGGCTCCGGCTTTTTACGCCCCGCAGA
  AGAAGTTCGGCCCTGTGGTGGCCCCAAAGCCCAAAGTGAATCCCTTCCGGCCCGGGGACAGCGAGCCTCC
  CCCGGCACCCGGGGCCCAGCGCGCACAGATGGGCCGGGTGGGCGAGATTCCCCCGCCGCCCCCGGAAGAC
  TTTCCCCTGCCTCCACCTCCCCTTGCTGGGGATGGCGACGATGCAGAGGGTGCTCTGGGAGGTGCCTTCC
  CGCCGCCCCCTCCCCCGATCGAGGAATCATTTCCCCCTGCGCCTCTGGAGGAGGAGATCTTCCCTTCCCC
  GCCGCCTCCTCCGGAGGAGGAGGGAGGGCCTGAGGCCCCCATACCGCCCCCACCACAGCCCAGGGAGAAG
  GTGAGCAGTATTGATTTGGAGATCGACTCTCTGTCCTCACTGCTGGATGACATGACCAAGAATGATCCTT
  TCAAAGCCCGGGTGTCATCTGGATATGTGCCCCCACCAGTGGCCACTCCATTCAGTTCCAAGTCCAGTAC
  CAAGCCTGCAGCCGGGGGCACAGCACCCCTGCCTCCTTGGAAGTCCCCTTCCAGCTCCCAGCCTCTGCCC
  CAGGTTCCGGCTCCGGCTCAGAGCCAGACACAGTTCCATGTTCAGCCCCAGCCCCAGCCCAAGCCTCAGG
  TCCAACTCCATGTCCAGTCCCAGACCCAGCCTGTGTCTTTGGCTAACACCCAGCCCCGAGGGCCCCCAGC
  CTCATCTCCGGCTCCAGCCCCTAAGTTTTCTCCAGTGACTCCTAAGTTTACTCCTGTGGCTTCCAAGTTC
  AGTCCTGGAGCCCCAGGTGGATCTGGGTCACAACCAAATCAAAAATTGGGGCACCCCGAAGCTCTTTCTG
  CTGGCACAGGCTCCCCTCAACCTCCCAGCTTCACCTATGCCCAGCAGAGGGAGAAGCCCCGAGTGCAGGA
  GAAGCAGCACCCCGTGCCCCCACCGGCTCAGAACCAAAACCAGGTGCGCTCCCCTGGGGCCCCAGGGCCC
  CTGACTCTGAAGGAGGTGGAGGAGCTGGAGCAGCTGACCCAGCAGCTAATGCAGGACATGGAGCATCCTC
  AGAGGCAGAATGTGGCTGTCAACGAACTCTGCGGCCGATGCCATCAACCCCTGGCCCGGGCGCAGCCAGC
  CGTCCGCGCTCTAGGGCAGCTGTTCCACATCGCCTGCTTCACCTGCCACCAGTGTGCGCAGCAGCTCCAG
  GGCCAGCAGTTCTACAGTCTGGAGGGGGCGCCGTACTGCGAGGGCTGTTACACTGACACCCTGGAGAAGT
  GTAACACCTGCGGGGAGCCCATCACTGACCGCATGCTGAGGGCCACGGGCAAGGCCTATCACCCGCACTG
  CTTCACCTGTGTGGTCTGCGCCCGCCCCCTGGAGGGCACCTCCTTCATCGTGGACCAGGCCAACCGGCCC
  CACTGTGTCCCCGACTACCACAAGCAGTACGCCCCGAGGTGCTCCGTCTGCTCTGAGCCCATCATGCCTG
  AGCCTGGCCGAGATGAGACTGTGCGAGTGGTCGCCCTGGACAAGAACTTCCACATGAAGTGTTACAAGTG
  TGAGGACTGCGGGAAGCCCCTGTCGATTGAGGCAGATGACAATGGCTGCTTCCCCCTGGACGGTCACGTG
  CTCTGTCGGAAGTGCCACACTGCTAGAGCCCAGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200960 representing NM_001010972
  Red=Cloning site Green=Tags(s)
  
  MAAPRPSPAISVSVSAPAFYAPQKKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPED
  FPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREK
  VSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLP
  QVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKF
  SPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGP
  LTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQ
  GQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRP
  HCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYKCEDCGKPLSIEADDNGCFPLDGHV
  LCRKCHTARAQT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001010972
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010972.2
• RefSeq Size: 2322 bp
• RefSeq ORF: 1719 bp
• Locus ID: 7791
• UniProt ID: Q15942
• Cytogenetics: 7q34
• Protein Pathways: Focal adhesion
• MW: 61.3 kDa
• Gene Summary: Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008]