SART1 (NM_005146) Human Untagged Clone

CAT#: SC319408

SART1 (untagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SART1
• Synonyms: Ara1; HAF; HOMS1; SART1259; SNRNP110; Snu66
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005146.4 CTGGGCTCGGCGGCAGCCGGGCTCGGAGTGGACGTGCCACTATGGGGTCGTCCAAGAAGC
  ATCGCGGAGAGAAGGAGGCGGCCGGGACGACGGCGGCGGCCGGCACCGGGGGTGCCACCG
  AGCAGCCGCCGCGGCACCGGGAACACAAAAAACACAAGCACCGGAGTGGCGGCAGTGGCG
  GTAGCGGTGGCGAACGACGGAAGCGGAGCCGGGAACGTGGGGGCGAGCGCGGGAGCGGGC
  GGCGCGGGGCCGAAGCTGAGGCCCGGAGCAGCACGCACGGGCGGGAGCGCAGCCAGGCAG
  AGCCCTCCGAGCGGCGCGTGAAGCGGGAGAAGCGCGATGACGGCTACGAGGCCGCTGCCA
  GCTCCAAAACTAGCTCAGGCGATGCCTCCTCACTCAGCATCGAGGAGACTAACAAACTCC
  GGGCAAAGTTGGGGCTGAAACCCTTGGAGGTTAATGCCATCAAGAAGGAGGCGGGCACCA
  AGGAGGAGCCCGTGACAGCTGATGTCATCAACCCTATGGCCTTGCGACAGCGAGAGGAGC
  TGCGGGAGAAGCTGGCAGCTGCCAAGGAGAAGCGCCTGCTGAACCAAAAGCTGGGGAAGA
  TAAAGACCCTAGGAGAGGATGACCCCTGGCTGGACGACACTGCAGCCTGGATCGAGAGGA
  GCCGGCAGCTGCAGAAGGAGAAGGACCTGGCAGAGAAGAGGGCCAAGTTACTGGAGGAGA
  TGGACCAAGAGTTTGGTGTCAGCACTCTGGTGGAGGAGGAGTTCGGGCAGAGGCGGCAGG
  ACCTGTACAGTGCCCGGGACCTGCAGGGCCTCACCGTGGAGCATGCCATTGATTCCTTCC
  GAGAAGGGGAGACAATGATTCTTACCCTCAAGGACAAAGGCGTGCTGCAGGAGGAGGAGG
  ACGTGCTGGTGAACGTGAACCTGGTGGATAAGGAGCGGGCAGAGAAAAATGTGGAGCTGC
  GGAAGAAGAAGCCTGACTACCTGCCCTATGCCGAGGACGAGAGCGTGGACGACCTGGCGC
  AGCAAAAACCTCGCTCTATCCTGTCCAAGTATGACGAAGAGCTTGAAGGGGAGCGGCCAC
  ATTCCTTCCGCTTGGAGCAGGGCGGCACGGCTGATGGCCTGCGGGAGCGGGAGCTGGAGG
  AGATCCGGGCCAAGCTGCGGCTGCAGGCTCAGTCCCTGAGCACAGTGGGGCCCCGGCTGG
  CCTCCGAATACCTCACGCCTGAGGAGATGGTGACCTTTAAAAAGACCAAGCGGAGGGTGA
  AGAAAATCCGCAAGAAGGAGAAGGAGGTAGTAGTGCGGGCAGATGACTTGCTGCCTCTCG
  GGGACCAGACTCAGGATGGGGACTTTGGTTCCAGACTGCGGGGACGGGGTCGCCGCCGAG
  TGTCCGAAGTGGAGGAGGAGAAGGAGCCTGTGCCTCAGCCCCTGCCGTCGGACGACACCC
  GAGTGGAGAACATGGACATCAGTGATGAGGAGGAAGGTGGAGCTCCACCGCCGGGGTCCC
  CGCAGGTGCTGGAGGAGGACGAGGCGGAGCTGGAGCTGCAGAAGCAGCTGGAGAAGGGAC
  GCCGGCTGCGACAGTTACAGCAGCTACAGCAGCTGCGAGACAGTGGCGAGAAGGTGGTGG
  AGATTGTGAAGAAGCTGGAGTCTCGCCAGCGGGGCTGGGAGGAGGATGAGGATCCCGAGC
  GGAAGGGGGCCATCGTGTTCAACGCCACGTCCGAGTTCTGCCGCACCTTGGGGGAGATCC
  CCACCTACGGGCTGGCTGGCAATCGCGAGGAGCAGGAGGAGCTCATGGACTTTGAACGGG
  ATGAGGAGCGCTCAGCCAACGGTGGCTCCGAATCTGACGGGGAGGAGAACATCGGCTGGA
  GCACGGTGAACCTGGACGAGGAGAAGCAGCAGCAGGATTTCTCTGCTTCCTCCACCACCA
  TCCTGGACGAGGAACCGATCGTGAATAGGGGGCTGGCAGCTGCCCTGCTCCTGTGTCAGA
  ACAAAGGGCTGCTGGAGACCACAGTGCAGAAGGTGGCCCGGGTGAAGGCCCCCAACAAGT
  CGCTGCCCTCAGCCGTGTACTGCATCGAGGATAAGATGGCCATCGATGACAAGTACAGCC
  GGAGGGAGGAATACCGAGGCTTCACACAGGACTTCAAGGAGAAGGACGGCTACAAACCCG
  ACGTTAAGATCGAATACGTGGATGAGACGGGCCGGAAACTCACACCCAAGGAGGCTTTCC
  GGCAGCTGTCGCACCGCTTCCATGGCAAGGGCTCAGGCAAGATGAAGACAGAGCGGCGGA
  TGAAGAAGCTGGACGAGGAGGCGCTCCTGAAGAAGATGAGCTCCAGCGACACGCCCCTGG
  GCACCGTGGCCCTGCTCCAGGAGAAGCAGAAGGCTCAGAAGACCCCCTACATCGTGCTCA
  GCGGCAGCGGCAAGAGCATGAACGCGAACACCATCACCAAGTGACAGCGCCCTCCCGCCC
  CGGCCCTGCCTCAACCTTCATATTAAATAAAGCTCCCTCCTTAAAAAAAAAAAAAAAAAA
  AAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005146
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005146.4, NP_005137.1
• RefSeq Size: 3601 bp
• RefSeq ORF: 2403 bp
• Locus ID: 9092
• UniProt ID: O43290
• Cytogenetics: 11q13.1
• Domains: SART-1
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. [provided by RefSeq, Jul 2008]