HSPA2 (NM_021979) Human Untagged Clone

CAT#: SC319383

HSPA2 (untagged)-Human heat shock 70kDa protein 2 (HSPA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSPA2
• Synonyms: HSP70-2; HSP70-3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021979.2 CTCTCTTGCCTGCCGCCATGTGCCTTTCGCCTTCTGCCGTGATTGTGAGGCCTCCCCAGC
  CATATGGAAGTTCAGGATGTCTGCCCGTGGCCCGGCTATCGGCATCGACCTGGGCACCAC
  CTATTCGTGCGTCGGGGTCTTCCAACATGGCAAGGTGGAGATCATCGCCAACGACCAGGG
  CAATCGCACCACCCCCAGCTACGTGGCCTTCACGGACACCGAGCGCCTCATCGGCGACGC
  CGCCAAGAACCAGGTGGCCATGAACCCCACCAACACCATCTTCGACGCCAAGAGGCTGAT
  TGGACGGAAATTCGAGGATGCCACAGTGCAGTCGGATATGAAACACTGGCCGTTCCGGGT
  GGTGAGCGAGGGAGGCAAGCCCAAAGTGCAAGTAGAGTACAAGGGGGAGACCAAGACCTT
  CTTCCCAGAGGAGATATCCTCCATGGTCCTCACGAAGATGAAGGAGATCGCGGAAGCCTA
  CCTGGGGGGCAAGGTGCACAGCGCGGTCATAACGGTCCCGGCCTATTTCAACGACTCGCA
  GCGCCAGGCCACCAAGGACGCAGGCACCATCACGGGGCTCAATGTGCTGCGCATCATCAA
  CGAGCCCACGGCGGCGGCCATCGCCTACGGCCTGGACAAGAAGGGCTGCGCGGGCGGCGA
  GAAGAACGTGCTCATCTTTGACCTGGGCGGTGGCACTTTCGACGTGTCCATCCTGACCAT
  CGAGGATGGCATCTTCGAGGTGAAGTCCACGGCCGGCGACACCCACCTGGGCGGTGAGGA
  CTTCGACAACCGCATGGTGAGCCACCTGGCGGAGGAGTTCAAGCGCAAGCACAAGAAGGA
  CATTGGGCCCAACAAGCGCGCCGTGAGGCGGCTGCGCACCGCTTGCGAGCGCGCCAAGCG
  CACCCTGAGCTCGTCCACGCAGGCGAGCATCGAGATCGACTCGCTCTACGAGGGCGTGGA
  CTTCTATACGTCCATCACGCGCGCCCGCTTCGAGGAGCTCAATGCCGACCTCTTTCGCGG
  GACCCTGGAGCCGGTGGAGAAGGCGCTGCGCGACGCCAAGCTGGACAAGGGCCAGATCCA
  GGAGATCGTGCTGGTGGGCGGCTCCACTCGTATCCCCAAGATCCAGAAGCTGCTGCAGGA
  TTTCTTCAACGGCAAGGAGCTGAACAAGAGCATCAACCCCGACGAGGCGGTGGCCTATGG
  CGCCGCGGTGCAGGCGGCCATCCTCATCGGCGACAAATCAGAGAATGTGCAGGACCTGCT
  GCTACTCGACGTGACCCCGTTGTCGCTGGGCATCGAGACAGCTGGCGGTGTCATGACCCC
  ACTCATCAAGAGGAACACCACGATCCCCACCAAGCAGACGCAGACCTTCACCACCTACTC
  GGACAACCAGAGCAGCGTACTGGTGCAGGTATACGAGGGCGAACGGGCCATGACCAAGGA
  CAATAACCTGCTGGGCAAGTTCGACCTGACCGGGATTCCCCCTGCGCCTCGCGGGGTCCC
  CCAAATCGAGGTTACCTTCGACATTGACGCCAATGGCATCCTTAACGTTACCGCCGCCGA
  CAAGAGCACCGGTAAGGAAAACAAAATCACCATCACCAATGACAAAGGTCGTCTGAGCAA
  GGACGACATTGACCGGATGGTGCAGGAGGCGGAGCGGTACAAATCGGAAGATGAGGCGAA
  TCGCGACCGAGTCGCGGCCAAAAACGCCCTGGAGTCCTATACCTACAACATCAAGCAGAC
  GGTGGAAGACGAGAAACTGAGGGGCAAGATTAGCGAGCAGGACAAAAACAAGATCCTCGA
  CAAGTGTCAGGAGGTGATCAACTGGCTCGACCGAAACCAGATGGCAGAGAAAGATGAGTA
  TGAACACAAGCAGAAAGAGCTCGAAAGAGTTTGCAACCCCATCATCAGCAAACTTTACCA
  AGGTGGTCCTGGCGGCGGCAGCGGCGGCGGCGGTTCAGGAGCCTCCGGGGGACCCACCAT
  CGAAGAAGTGGACTAAGCTTGCACTCAAGTCAGCGTAAACCTCTTTGCCTTTCTCTCTCT
  CTCTTTTTTTTTGTTTGTTTCTTTGAAATGTCCTTGTGCCAAGTACGAGATCTATTGTTG
  GAAGTCTTTGGTATATGCAAATGAAAGGAGAGGTGCAACAACTTAGTTTAATTATAAAAG
  TTCCAAAGTTTGTTTTTTAAAAACATTATTCGAGGTTTCTCTTTAATGCATTTTGCGTGT
  TTGCTGACTTGAGCATTTTTGATTAGTTCGTGCATGGAGATTTGTTTGAGATGAGAAACC
  TTAAGTTTGCACACCTGTTCTGTAGAAGCTTGGAAACAGTAAAATATATAGGAGCTTAAA
  TTGTTTATTTTTATGTACTACTTTAAAACTAAACTGAACATTGCAGTAATGTTAAGGACA
  GGTATACTTTTTGCAAACAAATGCATAAATGCAAATGTAAAGTAAAGCTGAAATTGATCT
  CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_021979
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021979.2, NP_068814.2
• RefSeq Size: 2496 bp
• RefSeq ORF: 1920 bp
• Locus ID: 3306
• UniProt ID: P54652
• Cytogenetics: 14q23.3
• Domains: HSP70
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome
• Gene Summary: Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release (PubMed:26865365). Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells (By similarity).[UniProtKB/Swiss-Prot Function]