POMGNT1 (NM_017739) Human Untagged Clone

CAT#: SC319371

POMGNT1 (untagged)-Human protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (POMGNT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POMGNT1
• Synonyms: gnT-I.2; GNTI.2; GnT I.2; LGMD2O; LGMDR15; MEB; MGAT1.2; RP76
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017739.2 GGCCGGGGCGGGGCCGCAAGCGGCATGGAGGAGGCGGAGGCCGCGGCGAGCCGGGCCGAG
  CAGTGAGGGCCCTAGCGGGGCCCGAGCGGGGCCCGGGGCCCCTAAGCCATTCCTGAAGTC
  ATGGGCTGGCCAGGACATTGGTGACCCGCCAATCCGGTATGGACGACTGGAAGCCCAGCC
  CCCTCATCAAGCCCTTTGGGGCTCGGAAGAAGCGGAGCTGGTACCTTACCTGGAAGTATA
  AACTGACAAACCAGCGGGCCCTGCGGAGATTCTGTCAGACAGGGGCCGTGCTTTTCCTGC
  TGGTGACTGTCATTGTCAATATCAAGTTGATCCTGGACACTCGGCGAGCCATCAGTGAAG
  CCAATGAAGACCCAGAGCCAGAGCAAGACTATGATGAGGCCCTAGGCCGCCTGGAGCCCC
  CACGGCGCAGAGGCAGTGGTCCCCGGCGGGTCCTGGACGTAGAGGTGTATTCAAGTCGCA
  GCAAAGTATATGTGGCAGTGGATGGCACCACGGTGCTGGAGGATGAGGCCCGGGAGCAGG
  GCCGGGGCATCCATGTCATTGTCCTCAACCAGGCCACGGGCCACGTGATGGCAAAACGTG
  TGTTTGACACGTACTCACCTCATGAGGATGAGGCCATGGTGCTATTCCTCAACATGGTAG
  CGCCCGGCCGAGTGCTCATCTGCACTGTCAAGGATGAGGGCTCCTTCCACCTCAAGGACA
  CAGCCAAGGCTCTGCTGAGGAGCCTGGGCAGCCAGGCTGGCCCTGCCCTGGGCTGGAGGG
  ACACATGGGCCTTCGTGGGACGAAAAGGAGGTCCTGTCTTCGGGGAGAAACATTCTAAAT
  CACCTGCCCTCTCTTCCTGGGGGGACCCAGTCCTGCTGAAGACAGATGTGCCATTGAGCT
  CAGCAGTAGAGGCAGAGTGCCACTGGGCAGACACAGAGCTGAACCGTCGCCGCCGGCGCT
  TCTGCAGCAAAGTTGAGGGCTATGGAAGTGTATGCAGCTGCAAGGACCCCACACCCATCG
  AGTTCAGCCCTGACCCACTCCCAGACAACAAGGTCCTCAATGTGCCTGTGGCTGTCATTG
  CAGGGAACCGACCCAATTACCTGTACAGGATGCTGCGCTCTCTGCTTTCAGCCCAGGGGG
  TGTCTCCTCAGATGATAACAGTTTTCATTGACGGCTACTATGAGGAACCCATGGATGTGG
  TGGCACTGTTTGGTCTGAGGGGCATCCAGCATACTCCCATCAGCATCAAGAATGCCCGCG
  TGTCTCAGCACTACAAGGCCAGCCTCACTGCCACTTTCAACCTGTTTCCGGAGGCCAAGT
  TTGCTGTGGTTCTGGAAGAGGACCTGGACATTGCTGTGGATTTTTTCAGTTTCCTGAGCC
  AATCCATCCACCTACTGGAGGAGGATGACAGCCTGTACTGCATCTCTGCCTGGAATGACC
  AGGGGTATGAACACACGGCTGAGGACCCAGCACTACTGTACCGTGTGGAGACCATGCCTG
  GGCTGGGCTGGGTGCTCAGGAGGTCCTTGTACAAGGAGGAGCTTGAGCCCAAGTGGCCTA
  CACCGGAAAAGCTCTGGGATTGGGACATGTGGATGCGGATGCCTGAACAACGCCGGGGCC
  GAGAGTGCATCATCCCTGACGTTTCCCGATCCTACCACTTTGGCATCGTCGGCCTCAACA
  TGAATGGCTACTTTCACGAGGCCTACTTCAAGAAGCACAAGTTCAACACGGTTCCAGGTG
  TCCAGCTCAGGAATGTGGACAGTCTGAAGAAAGAAGCTTATGAAGTGGAAGTTCACAGGC
  TGCTCAGTGAGGCTGAGGTTCTGGACCACAGCAAGAACCCTTGTGAAGACTCTTTCCTGC
  CAGACACAGAGGGCCACACCTACGTGGCCTTTATTCGAATGGAGAAAGATGATGACTTCA
  CCACCTGGACCCAGCTTGCCAAGTGCCTCCATATCTGGGACCTGGATGTGCGTGGCAACC
  ATCGGGGCCTGTGGAGATTGTTTCGGAAGAAGAACCACTTCCTGGTGGTGGGGGTCCCGG
  CTTCCCCCTACTCAGTGAAGAAGCCACCCTCAGTCACCCCAATTTTCCTGGAGCCACCCC
  CAAAGGAGGAGGGAGCCCCAGGAGCCCCAGAACAGACATGAGACCTCCTCCAGGACCCTG
  CGGGGCTGGGTACTGTGTACCCCCAGGCTGGCTAGCCCTTCCCTCCATCCTGTAGGATTT
  TGTAGATGCTGGTAGGGGCTGGGGCTACCTTGTTTTTAACATGAGACTTAATTACTAACT
  CCAAGGGGAGGGTTCCCCTGCTCCAACACCCCGTTCCTGAGTTAAAAGTCTATTTATTTA
  CTTCCTTGTTGGAGAAGGGCAGGAGAGTACCTGGGAATCATTACGATCCCTAGCAGCTCA
  TCCTGCCCTTTGAATACCCTCACTTTCCAGGCCTGGCTCAGAATCTAACCTATTTATTGA
  CTGTCCTGAGGGCCTTGAAAACAGGCCGAACCTGGAGGGCCTGGATTTCTTTTTGGGCTG
  GAATGCTGCCCTGAGGGTGGGGCTGGCTCTTACTCAGGAAACTGCTGTGCCCAACCCATG
  GACAGGCCCAGCTGGGGCCCACATGCTGACACAGACTCACTCAGAGACCCTTAGACACTG
  GACCAGGCCTCCTCTCAGCCTTCTCTTTGTCCAGATTTCCAAAGCTGGATAAGTTGGTCA
  TTGATTAAAAAAGGAGAAGCCCTCTGAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_017739
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017739.2, NP_060209.2
• RefSeq Size: 2753 bp
• RefSeq ORF: 1983 bp
• Locus ID: 55624
• UniProt ID: Q8WZA1
• Cytogenetics: 1p34.1
• Domains: GNT-I
• Protein Pathways: O-Mannosyl glycan biosynthesis
• Gene Summary: This gene encodes a type II transmembrane protein that resides in the Golgi apparatus. It participates in O-mannosyl glycosylation and is specific for alpha linked terminal mannose. Mutations in this gene may be associated with muscle-eye-brain disease and several congenital muscular dystrophies. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the shorter isoform (1).