PISD (NM_014338) Human Untagged Clone

CAT#: SC319343

PISD (untagged)-Human phosphatidylserine decarboxylase (PISD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PISD
• Synonyms: DJ858B16; dJ858B16.2; LIBF; PSD; PSDC; PSSC
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014338.3 GGAAGCTGGAGCCACCTGCGGCTCTGAGAGGGACAGACGGTGCTCAGCCTGTGTCCTGGG
  GCAAAGCTGGATTCATTTTCCTCCCTGGCGATCTTCTGGGGCCCACAGCTGGTGATATGG
  AGGCACTGACACCCTGGTCTCGGCGCTTGTTTTAGAAGAGGCAGAACAGTGATAAAGACA
  AGGATCTAAATGGGCAAGGACGCCCTGCCCTCACTGGGCTCACTGGCAGCCCTGACCCTC
  TATTCTGCACCCTGTGAGCCACCGAGACTGAACCAAACCCAGGAAGCGGCGCCACCAACC
  AGCCTCGCAGAGGCAGAGGCCCTGGAGCAGTGACAGCACAGGAGATCTGCTGGCTCGGGG
  GAAGGGGAGGAAAGAGGAAAACAAAGGGAGTGATCGAGTGAGCCTCGCCCCGCCCTCCCT
  GCGCCGGCCCATGATGTGTCAGTCAGAGGCGCGGCAAGGACCAGAGCTCCGCGCGGCGAA
  ATGGTTGCACTTCCCCCAGCTGGCCCTGAGGCGGAGGCTGGGGCAGCTGAGCTGCATGTC
  CAGACCCGCTCTGAAACTGCGCTCCTGGCCCTTGACCGTCCTCTACTACCTCCTGCCCTT
  CGGCGCCCTCAGACCGCTCAGCCGGGTGGGATGGAGGCCCGTAAGCAGGGTGGCTTTGTA
  CAAGTCAGTGCCAACGCGCTTGCTGTCACGGGCCTGGGGTCGCCTCAATCAGGTGGAGCT
  GCCACACTGGCTGCGCAGGCCCGTCTACAGCCTGTACATCTGGACGTTTGGGGTGAACAT
  GAAAGAGGCCGCTGTGGAGGACCTGCATCACTACCGCAACCTCAGCGAGTTCTTCCGGCG
  CAAGCTGAAGCCGCAGGCCCGGCCTGTCTGTGGCCTGCACAGCGTGATTAGCCCATCGGA
  TGGAAGGATCCTCAACTTTGGGCAGGTGAAGAACTGTGAGGTGGAGCAGGTAAAGGGGGT
  CACCTACTCCCTGGAGTCGTTCCTGGGCCCGCGTATGTGCACAGAGGACCTGCCCTTCCC
  ACCAGCCGCGTCGTGTGACTCCTTCAAGAACCAGCTGGTCACCCGGGAAGGGAATGAGCT
  CTATCACTGTGTCATCTACCTGGCCCCTGGGGACTACCACTGCTTCCACTCCCCCACCGA
  CTGGACTGTGTCCCACCGGCGCCACTTCCCAGGCTCCCTGATGTCAGTGAACCCTGGCAT
  GGCTCGCTGGATCAAAGAGCTCTTCTGCCATAACGAGCGGGTGGTCCTGACGGGGGACTG
  GAAACATGGCTTCTTCTCACTGACAGCTGTGGGGGCCACCAACGTGGGCTCCATTCGCAT
  CTACTTTGACCGGGACCTGCACACAAACAGCCCAAGGCACAGCAAGGGCTCCTACAATGA
  CTTCAGCTTCGTGACGCACACCAATAGAGAGGGCGTCCCCATGCGTAAGGGCGAGCACCT
  GGGCGAGTTCAACCTGGGCTCCACCATCGTGCTCATCTTCGAGGCCCCCAAGGACTTCAA
  TTTCCAGCTGAAAACAGGACAGAAAATCCGCTTTGGGGAAGCCCTGGGCTCGCTCTAGAG
  TCTCTTTCCTGATTATGGCTGCTAAGGGATCTTTTCCAAACAGAGTGAGGGTCTTTTCAA
  GAGGGAGGCCCATGAGGCCATCCAGGTAAGGGCCTGCCTCAGCGTGGTTGGGAGTCTGAC
  CAGGTAGGACTTGAATGATTCGGCTACCACCTGTTCCAGAGGTGCAGACAAGAGGTGGCG
  AGAGCCCCCATCATGCCCCTCAACCTATCCCGTTCCTTCTGCCTACAAATAAAAAGTGCA
  GGCTGGAATGATCTCAGTCACATTTGGATCTTTTTAAACACTGTATAGACGGAAGAGCCT
  GCATTCCTGACCGAACCTTCAGTTGGTCTCGGTTGTCGTTTTTTCTTGCTGCTCCTCCCC
  CCATCACCTGAGCTGTTTTCTGTTGGCCCCTTTTGTTTTTTGGCCTTAACGCTCCTGCTG
  CACAGGGTGAGGTGCCTCCTTGGCACAGACTGTGGATGCCTCTCCCCCAGCAGAGCCACA
  CAGCCTTCGTGACAACTGCTTTCCGTTCCCACATTCACCTCATCCTGCTCTTTAGAAAAA
  GCAGTCTTTGTGCTTGTGGCTGAACGCATCACCCTGGACTCTGCTAGTGTCTTCTGAGGA
  CACTGATGACACTGATTAATGATACAGACCTTTGCAGGACCTGATGAGTGACCCTTCTGG
  AGCTGGCCAGGTCCTCTGCAGCAGGCAAGACCAATCAATCACTGAACCTGCCTCATGGCA
  CCAGAGTGAACAGGGCAGGCAGGTAGTAGGCCCAGCTGGGGAAATGGGAGAGTTCCTGTC
  CCCCTCCACATATCCCTACATGAAATATGGGAAAGTTGCTGCTATTGATTCAGGGTCTGT
  CTTGGAGGCAGAGGACCCTTGGTGGATAGTTGGTCAGTGCCTGGAAAACCTGTCCCAGTT
  TATCAGGAACGCAGGCCTGGGGAGCCCCCAGTGGCGGGGACAGGGCCAGATTTCATGTTG
  ACCCTGGGGATGCTGTGAATTTCTCCTGCAGGAGAGACATCATTGAATTTTTTCAACTGT
  ATCAGTAGCACAGTATTTTTGTATGAAAAGTGGGAGACTTCTGAACAGTAATTCATTTAA
  TTGCAAAGCATTTTGAAATAAAAAAAATCAAACTTAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_014338
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014338.3, NP_055153.1
• RefSeq Size: 2693 bp
• RefSeq ORF: 1128 bp
• Locus ID: 23761
• UniProt ID: Q9UG56
• Cytogenetics: 22q12.2
• Domains: PS_Dcarbxylase
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene catalyzes the conversion of phosphatidylserine to phosphatidylethanolamine in the inner mitochondrial membrane. The encoded protein is active in phospholipid metabolism and interorganelle trafficking of phosphatidylserine. [provided by RefSeq, May 2016]
  Transcript Variant: This variant (5), as well as variants 6, 7, 8, and 9, encodes isoform d.