Phospholipase C beta 2 (PLCB2) (NM_004573) Human Untagged Clone

CAT#: SC318538

PLCB2 (untagged)-Human phospholipase C, beta 2 (PLCB2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Phospholipase C beta 2
• Synonyms: PLC-beta-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318538 representing NM_004573.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTCTGCTCAACCCTGTCCTGCTGCCCCCCAAGGTGAAGGCCTATCTGAGCCAAGGGGAGCGCTTC
  ATCAAATGGGATGATGAAACTACAGTTGCCTCTCCAGTTATCCTCCGTGTGGATCCTAAGGGCTACTAC
  TTATACTGGACGTATCAAAGTAAGGAGATGGAGTTTCTGGATATCACCAGCATCCGGGATACTCGCTTT
  GGGAAGTTTGCCAAGATGCCCAAGAGCCAGAAGCTCCGGGACGTCTTCAACATGGACTTTCCTGATAAC
  AGTTTCCTGCTGAAGACACTCACGGTGGTGTCCGGCCCGGACATGGTGGACCTCACCTTCCACAACTTC
  GTCTCCTACAAGGAGAACGTGGGCAAGGCCTGGGCTGAGGACGTACTGGCCCTAGTCAAACATCCGCTG
  ACGGCCAACGCCTCCCGCAGCACCTTCCTGGACAAGATCCTTGTGAAGCTCAAGATGCAGCTCAACTCT
  GAAGGGAAGATTCCGGTGAAGAACTTTTTCCAGATGTTTCCTGCTGACCGCAAGCGGGTGGAAGCTGCT
  CTCAGTGCCTGCCACCTCCCCAAAGGCAAAAATGACGCCATCAATCCTGAGGACTTCCCAGAACCTGTC
  TACAAGAGTTTCCTCATGAGCCTCTGTCCTCGGCCAGAAATAGATGAGATCTTCACTTCTTACCATGCT
  AAGGCCAAACCCTACATGACGAAGGAGCACCTGACCAAATTCATCAACCAGAAACAGCGGGACTCCCGG
  CTTAACTCCCTGCTGTTCCCGCCAGCACGGCCTGACCAGGTGCAGGGCCTCATCGACAAGTATGAGCCC
  AGTGGCATCAATGCACAGAGGGGCCAGCTGTCACCTGAAGGCATGGTCTGGTTTCTCTGTGGGCCAGAG
  AACAGCGTGCTGGCCCAGGACAAGCTGCTGCTCCACCACGACATGACGCAGCCACTCAATCATTACTTC
  ATCAACTCGTCCCACAACACCTACCTGACAGCCGGCCAGTTCTCAGGCCTCTCCTCGGCTGAGATGTAC
  CGCCAGGTGCTGCTCTCTGGCTGCCGTTGCGTGGAGCTAGACTGCTGGAAGGGGAAACCCCCTGACGAG
  GAGCCCATTATCACCCATGGCTTCACCATGACCACAGACATCTTCTTCAAAGAAGCAATTGAGGCTATT
  GCAGAAAGCGCCTTTAAGACCTCCCCCTATCCCATCATCCTGTCGTTTGAGAACCATGTGGACTCACCC
  CGCCAGCAGGCTAAGATGGCTGAGTATTGCCGGACGATCTTTGGGGATATGCTGCTCACAGAGCCCCTG
  GAAAAGTTCCCACTAAAACCAGGTGTCCCCCTGCCCAGCCCTGAGGATCTCAGGGGCAAGATCCTCATC
  AAGAACAAGAAGAACCAGTTTTCTGGCCCCACCTCCTCCAGTAAGGATACTGGTGGGGAGGCTGAGGGC
  AGCAGCCCACCCAGTGCCCCTGCAGGTGAGGGCACAGTGTGGGCTGGCGAGGAAGGGACTGAGCTGGAG
  GAGGAGGAGGTGGAAGAGGAAGAGGAGGAGGAGTCAGGAAACCTGGATGAAGAAGAGATTAAGAAGATG
  CAGTCGGATGAGGGCACAGCGGGCCTGGAAGTGACGGCTTATGAGGAGATGTCCAGCCTAGTCAATTAC
  ATCCAGCCCACCAAGTTCGTCTCCTTTGAGTTCTCTGCCCAAAAGAACCGAAGTTATGTCATCTCGTCC
  TTCACAGAGCTCAAGGCATATGACCTGCTCTCCAAGGCCTCGGTGCAGTTTGTGGACTACAACAAGCGC
  CAGATGAGCCGCATTTACCCCAAGGGAACCCGCATGGACTCCTCCAACTACATGCCCCAGATGTTCTGG
  AATGCTGGATGCCAGATGGTTGCCCTCAACTTCCAGACGATGGACTTGCCCATGCAGCAGAACATGGCA
  GTATTTGAGTTCAACGGGCAGAGCGGCTACCTCCTCAAGCATGAGTTCATGCGCCGGCCGGACAAGCAG
  TTCAACCCCTTCTCAGTGGACCGCATCGACGTGGTGGTGGCCACCACCCTTTCCATTACGGTGATCTCT
  GGGCAGTTCCTGTCAGAACGCAGCGTGCGCACCTATGTAGAAGTGGAGCTGTTTGGCCTTCCTGGGGAC
  CCCAAGAGGCGCTATCGAACTAAGCTGTCACCCAGTACTAACTCCATCAATCCTGTCTGGAAGGAGGAG
  CCCTTTGTCTTTGAGAAGATCTTGATGCCTGAGCTGGCCTCCCTCAGAGTGGCTGTGATGGAGGAAGGC
  AACAAGTTTCTTGGACACCGCATCATCCCCATCAATGCCCTAAATTCTGGGTACCACCACCTGTGCCTG
  CACAGTGAGAGCAACATGCCCCTCACCATGCCTGCGCTCTTCATCTTCCTGGAGATGAAGGACTACATA
  CCTGGTGCTTGGGCAGATCTCACTGTGGCCCTCGCCAACCCCATTAAGTTCTTCAGTGCCCATGACACG
  AAGTCTGTGAAGCTCAAGGAGGCCATGGGAGGTCTGCCTGAGAAGCCCTTCCCACTGGCGAGTCCAGTT
  GCCAGCCAGGTCAATGGGGCGTTGGCCCCAACGAGCAATGGGTCACCAGCAGCCAGGGCCGGGGCCAGG
  GAAGAGGCTATGAAAGAAGCTGCGGAGCCGCGGACCGCCAGCCTGGAGGAGCTCCGGGAGCTAAAGGGC
  GTGGTGAAGCTGCAGCGGCGGCACGAGAAGGAGCTGCGAGAGTTGGAGCGGCGCGGAGCGCGGCGCTGG
  GAGGAGCTGCTGCAGCGGGGCGCGGCGCAGCTGGCGGAGCTCGGGCCACCGGGCGTGGGGGGCGTCGGG
  GCCTGCAAGCTCGGTCCCGGCAAGGGCTCTCGCAAGAAGAGGAGCCTGCCCCGCGAGGAGAGCGCCGGA
  GCCGCGCCGGGCGAGGGCCCTGAGGGCGTGGACGGGCGCGTGCGGGAGCTGAAAGACAGGCTGGAGCTG
  GAGCTGCTGCGGCAGGGCGAGGAGCAGTACGAGTGCGTTCTGAAGCGCAAGGAGCAGCACGTGGCCGAG
  CAAATCTCCAAAATGATGGAGCTGGCCAGAGAGAAACAGGCGGCAGAGCTGAAGGCCCTGAAGGAGACG
  TCGGAGAACGACACCAAAGAGATGAAGAAAAAGCTGGAGACAAAGAGACTGGAGCGGATCCAGGGCATG
  ACCAAAGTCACCACAGACAAGATGGCCCAGGAGAGGTTGAAGAGAGAGATTAACAACTCCCACATCCAG
  GAAGTAGTGCAGGTGATCAAGCAGATGACGGAGAACTTGGAGAGGCACCAGGAGAAGCTGGAGGAGAAG
  CAGGCGGCTTGCCTGGAACAGATACGGGAGATGGAAAAGCAGTTCCAGAAGGAGGCGCTGGCAGAGTAC
  GAGGCCAGGATGAAGGGTCTGGAGGCAGAGGTGAAGGAGTCGGTGAGGGCCTGCCTCAGGACCTGCTTT
  CCCTCCGAGGCCAAGGACAAGCCTGAGAGGGCCTGCGAGTGCCCCCCAGAGCTGTGTGAGCAGGACCCA
  CTCATAGCAAAGGCAGATGCCCAGGAGAGCCGCCTCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004573
• Insert Size: 3558 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004573.2
• RefSeq Size: 4694 bp
• RefSeq ORF: 3558 bp
• Locus ID: 5330
• UniProt ID: Q00722
• Cytogenetics: 15q15.1
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway, Chemokine signaling pathway, Gap junction, GnRH signaling pathway, Huntington's disease, Inositol phosphate metabolism, Long-term depression, Long-term potentiation, Melanogenesis, Metabolic pathways, Phosphatidylinositol signaling system, Taste transduction, Vascular smooth muscle contraction, Wnt signaling pathway
• MW: 134 kDa
• Gene Summary: The protein encoded by this gene is a phosphodiesterase that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to the second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol. The encoded protein is activated by G proteins and has been shown to be involved in the type 2 taste receptor signal transduction pathway. In addition, nuclear factor kappa B can regulate the transcription of this gene, whose protein product is also an important regulator of platelet responses. [provided by RefSeq, Jan 2017]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).