TMEM146 (CATSPERD) (NM_152784) Human Untagged Clone

CAT#: SC318282

CATSPERD (untagged)-Human transmembrane protein 146 (TMEM146)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CATSPERD
• Synonyms: TMEM146
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318282 representing NM_152784.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGATGTTGATGCTGGTGGCGGCTGTGACCATGTGGCTCCGACCGCTGGTCACAGCTCAGCTCTGT
  CGTTCTCGCACAGTGAGGACAGGAAAAGTGTTTAATCTGATACAGGACGTTCAAGGGGACCGCCTGTAT
  TTTCATCCTACAACAACACGCTTGATTAAACATCCTTGCGAGAAAAATATAGCACTATATCTAGGGAAA
  CAAGTTTTTTTCACAATGGATAACTTTGAGACTAGTCTCCTTCCATTTACCATCCCTACATCAATGCAG
  GTCGGCGTACCAGAAGTGACATCAGCACATTTTGCTGGTTCGTTATTGCTGTTAGTAGTGGATCAAAAA
  GTCTATATTTATGATTATGAAAATAATTCTTGGAGCATGTCTTTAGGTATAAAACACCCTGTTACACAT
  GTCTCTGGTGATAATTGTTGTTATACTGGAAGTTTGTTTTGTGTGCATGTCAGTAATTTGGTTTTTGCA
  TATTTCCGTGGAGATCAGATATCCCAGACTTATATATATTATTCAAATACTGGGGGATTCAGTTTTTGG
  AAGTATCACTATGACAGACAGGCAGAAATCATTGGGTCTTTAGGCGGAATCTTCCACTTTTTTTCTTTG
  TCACAGGTTGCGATGCTTGTAGTGAATCAAGGGAAGGGCATGTTCAAGTACTCAGATCACCCCCTCAAC
  CGGAGTTTCGGGCTGTCTTTTGACTATAATGGGACTCTAGACATCCTCATCGCCCCCGGCCAGAGAGGC
  ATCCTGCTCCTATGGTTTGAGAACAGCCTGTTGTTTTCCCATAATGCAGGTCAGCTCGTCGACACCGTC
  CGGGTGAAAAAAGGAGACCAGACCTTGTTTTCTTCCATTTTTGAAGCCAAGATCACCATCCACAACATT
  GCTGTCACTGAAAATGAACTGGCAGTTATAACTCGGGAGGATAATTTGTATTATGGCAATCTGGGCATC
  GTGCCAAGTTCCATAATCAAATTTGCAGACCAATACATCTGGTCAGAAGACGTGGCCCTGATGTTCAGG
  AGCCCAGGGACTCTGGAAATACTGACCCCACTGCGTGACACAGCCTTTCCAGCTTTTGATTTCCAGAAG
  TGCCTCGTGAATATCCAGGCGCTTCTCATGGACCCTGAACTCCACGTTGGAAAGTGCAAGATAGAGTTT
  CTGACAGGAGAATTTATATACAGGATGTATACCATTGACATGCACAGCCAGCTGGAATTGACTGCTTCG
  TTGATACCCCAGCCAGGCACATCCCTGATTCCTCTGGTGATGGTGAGCAACCCCCACTCCCTGGGGTTC
  CAGGCCACCTTCTACGAGAACGGTTACACATCAGATGGGAACACCAAGTACAAACTGGATATTTTCCTA
  AAACAGCAGCAGCACTGGGGCAGGACCGACTCCAACTTCACTTCCAGTTTAAAGAAAGCCACCATGTCT
  ACCTTAACTGTGGACATAGCAAACAAGGAAATTTCATGTGTGGATATCAAGCCACTGTCGACACTGATT
  TCAGTTGGCTGCGACCTGGATAAAAAGATCGTCATCCAGAACAAAGTTTCCGCCTGTTCCATGGGCATC
  CTGGACCCCTTGACCCTGCAAGACAATTACAGCTTCATCATCGAGAAGGAATTCTACGACCCCGGCTTC
  CAGGGGCAGCAGTCCTCCGAGGACCTGCACGTGTTTTACTCCTACCAGCAGCTGGGCTGTCCTCTCCTC
  GTCTACTATGACACCCTATGGAAGCCCGTGGTGGAGCTGTGGCGAAAAGACAGTTTCCAGGAGGTCATC
  GACGCCGAGTATGTGTTACTGGAGGTGAACGGGCAGTTCTCATACTCCTATTCCCTGACGGCCCAGTCG
  GCCATGTGTACCTCCCAGCCGCAGAACTGGACCACCATGATAAAGGAATTCGGGGGGCCCTTCTTCTGG
  AACAGAGAGAACTATGTGAGCTGCCACGACCCCAACAACAATGCCCCTTTGAGGTGGCCAGACGTCCAG
  TATCAGATCTTGGGCGGCCGGACAGCAAACCAGATCATTTTCGGCCACAATGGCTTTTATGTCTTCTAC
  ATTTCGATCGTGGATCCGTACTACAGCTACTGTCAACTGGAGACCATCTTTAGCATCTACGTGTATGGA
  GCATTCCCCGTGCAGCTGGTCTCTGCTGGAGTCGTCATCCTACTGATCATCTCCAGCATCCTGGGGTCC
  GTTTGGCTGGCCTACAAGACCCCCAAGCTGCTACGCACAGCACGCGGCCGCAGGATCAAGAAGTGTGCG
  ACACAGCTGTGTAGGAGATGCAAGACGGTCTGCCAGTTCAGGGCCTCAGCCACAGCCAGGGCAGGCACA
  GAGCCCCCGGGACGCCACCGCACTCCTCACGGAGGCAGGTCTGACCACTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_152784
• Insert Size: 2397 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152784.3
• RefSeq Size: 2528 bp
• RefSeq ORF: 2397 bp
• Locus ID: 257062
• UniProt ID: Q86XM0
• Cytogenetics: 19p13.3
• Protein Families: Transmembrane
• MW: 90.5 kDa
• Gene Summary: Auxiliary component of the CatSper complex, a complex involved in sperm cell hyperactivation. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization. Required for CATSPER1 stability before intraflagellar transport and/or incorporation of the CatSper complex channel into the flagellar membrane.[UniProtKB/Swiss-Prot Function]