TMEM146 (CATSPERD) (NM_152784) Human Tagged ORF Clone

CAT#: RG207400

CATSPERD (tGFP-tagged) - Human transmembrane protein 146 (TMEM146)

Product Images

Circular map for RG207400

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TMEM146
• Synonyms: TMEM146
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207400 representing NM_152784
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGTGGCGGCTGTGACCATGTGGCTCCGACCGCTGGTCACAGCTCAGCTCTGTCGTTCTCGCACAG
  TGAGGACAGGAAAAGTGTTTAATCTGATACAGGACGTTCAAGGGGACCGCCTGTATTTTCATCCTACAAC
  AACACGCTTGATTAAACATCCTTGCGAGAAAAATATAGCACTATATCTAGGGAAACAAGTTTTTTTCACA
  ATGGATAACTTTGAGACTAGTCTCCTTCCATTTACCATCCCTACATCAATGCAGGTCGGCGTACCAGAAG
  TGACATCAGCACATTTTGCTGGTTCGTTATTGCTGTTAGTAGTGGATCAAAAAGTCTATATTTATGATTA
  TGAAAATAATTCTTGGAGCATGTCTTTAGGTATAAAACACCCTGTTACACATGTCTCTGGTGATAATTGT
  TGTTATACTGGAAGTTTGTTTTGTGTGCATGTCAGTAATTTGGTTTTTGCATATTTCCGTGGAGATCAGA
  TATCCCAGACTTATATATATTATTCAAATACTGGGGGATTCAGTTTTTGGAAGTATCACTATGACAGACA
  GGCAGAAATCATTGGGTCTTTAGGCGGAATCTTCCACTTTTTTTCTTTGTCACAGGTTGCGATGCTTGTA
  GTGAATCAAGGGAAGGGCATGTTCAAGTACTCAGATCACCCCCTCAACCGGAGTTTCGGGCTGTCTTTTG
  ACTATAATGGGACTCTAGACATCCTCATCGCCCCCGGCCAGAGAGGCATCCTGCTCCTATGGTTTGAGAA
  CAGCCTGTTGTTTTCCCATAATGCAGGTCAGCTCGTCGACACCGTCCGGGTGAAAAAAGGAGACCAGACC
  TTGTTTTCTTCCATTTTTGAAGCCAAGATCACCATCCACAACATTGCTGTCACTGAAAATGAACTGGCAG
  TTATAACTCGGGAGGATAATTTGTATTATGGCAATCTGGGCATCGTGCCAAGTTCCATAATCAAATTTGC
  AGACCAATACATCTGGTCAGAAGACGTGGCCCTGATGTTCAGGAGCCCAGGGACTCTGGAAATACTGACC
  CCACTGCGTGACACAGCCTTTCCAGCTTTTGATTTCCAGAAGTGCCTCGTGAATATCCAGGCGCTTCTCA
  TGGACCCTGAACTCCACGTTGGAAAGTGCAAGATAGAGTTTCTGACAGGAGAATTTATATACAGGATGTA
  TACCATTGACATGCACAGCCAGCTGGAATTGACTGCTTCGTTGATACCCCAGCCAGGCACATCCCTGATT
  CCTCTGGTGATGGTGAGCAACCCCCACTCCCTGGGGTTCCAGGCCACCTTCTACGAGAACGGTTACACAT
  CAGATGGGAACACCAAGTACAAACTGGATATTTTCCTAAAACAGCAGCAGCACTGGGGCAGGACCGACTC
  CAACTTCACTTCCAGTTTAAAGAAAGCCACCATGTCTACCTTAACTGTGGACATAGCAAACAAGGAAATT
  TCATGTGTGGATATCAAGCCACTGTCGGCACTGATTTCAGTTGGCTGCGACCTGGATAAAAAGATCGTCA
  TCCAGAACAAAGTTTCCGCCTGTTCCATGGGCATCCTGGACCCCTTGACCCTGCAAGACAATTACAGCTT
  CATCATCGAGAAGGAATTCTACGACCCCGGCTTCCAGGGGCAGCAGTCCTCCGAGGACCTGCACGTGTTT
  TACTCCTACCAGCAGCTGGGCTGTCCTCTCCTCGTCTACTATGACACCCTATGGAAGCCCGTGGTGGAGC
  TGTGGCGAAAAGACAGTTTCCAGGAGGTCATCGACGCCGAGTATGTGTTACTGGAGGTGAACGGGCAGTT
  CTCATACTCCTATTCCCTGACGGCCCAGTCGGCCATGTGTACCTCCCAGCCGCAGAACTGGACCACCATG
  ATAAAGGAATTCGGGGGGCCCTTCTTCTGGAACAGAGAGAACTATGTGAGCTGCCACGACCCCAACAACA
  ATGCCCCTTTGAGGTGGCCAGACGTCCAGTATCAGATCTTGGGCGGCCGGACAGCAAACCAGATCATTTT
  CGGCCACAATGGCTTTTATGTCTTCTACATTTCGATCGTGGATCCGTACTACAGCTACTGTCAACTGGAG
  ACCATCTTTAGCATCTACGTGTATGGAGCATTCCCCGTGCAGCTGGTCTCTGCTGGAGTCGTCATCCTAC
  TGATCATCTCCAGCATCCTGGGGTCCGTTTGGCTGGCCTACAAGACCCCCAAGCTGCTACGCACAGCACG
  CGGCCGCAGGATCAAGAAGTGTGCGACACAGCTGTGTAGGAGATGCAAGACGGTCTGCCAGTTCAGGGCC
  TCAGCCACAGCCAGGGCAGGCACAGAGCCCCCGGGACGCCACCGCACTCCTCACGGAGGCAGGTCTGACC
  AC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207400 representing NM_152784
  Red=Cloning site Green=Tags(s)
  
  MLVAAVTMWLRPLVTAQLCRSRTVRTGKVFNLIQDVQGDRLYFHPTTTRLIKHPCEKNIALYLGKQVFFT
  MDNFETSLLPFTIPTSMQVGVPEVTSAHFAGSLLLLVVDQKVYIYDYENNSWSMSLGIKHPVTHVSGDNC
  CYTGSLFCVHVSNLVFAYFRGDQISQTYIYYSNTGGFSFWKYHYDRQAEIIGSLGGIFHFFSLSQVAMLV
  VNQGKGMFKYSDHPLNRSFGLSFDYNGTLDILIAPGQRGILLLWFENSLLFSHNAGQLVDTVRVKKGDQT
  LFSSIFEAKITIHNIAVTENELAVITREDNLYYGNLGIVPSSIIKFADQYIWSEDVALMFRSPGTLEILT
  PLRDTAFPAFDFQKCLVNIQALLMDPELHVGKCKIEFLTGEFIYRMYTIDMHSQLELTASLIPQPGTSLI
  PLVMVSNPHSLGFQATFYENGYTSDGNTKYKLDIFLKQQQHWGRTDSNFTSSLKKATMSTLTVDIANKEI
  SCVDIKPLSALISVGCDLDKKIVIQNKVSACSMGILDPLTLQDNYSFIIEKEFYDPGFQGQQSSEDLHVF
  YSYQQLGCPLLVYYDTLWKPVVELWRKDSFQEVIDAEYVLLEVNGQFSYSYSLTAQSAMCTSQPQNWTTM
  IKEFGGPFFWNRENYVSCHDPNNNAPLRWPDVQYQILGGRTANQIIFGHNGFYVFYISIVDPYYSYCQLE
  TIFSIYVYGAFPVQLVSAGVVILLIISSILGSVWLAYKTPKLLRTARGRRIKKCATQLCRRCKTVCQFRA
  SATARAGTEPPGRHRTPHGGRSDH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152784
• ORF Size: 2394 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152784.3, NP_689997.3
• RefSeq Size: 2528 bp
• RefSeq ORF: 2397 bp
• Locus ID: 257062
• UniProt ID: Q86XM0
• Cytogenetics: 19p13.3
• Protein Families: Transmembrane
• Gene Summary: Auxiliary component of the CatSper complex, a complex involved in sperm cell hyperactivation. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization. Required for CATSPER1 stability before intraflagellar transport and/or incorporation of the CatSper complex channel into the flagellar membrane.[UniProtKB/Swiss-Prot Function]