CNO6L (CNOT6L) (NM_144571) Human Untagged Clone

CAT#: SC317951

CNOT6L (untagged)-Human CCR4-NOT transcription complex, subunit 6-like (CNOT6L)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CNO6L
• Synonyms: CCR4b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317951 representing NM_144571.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGACTAATAGGGATGCCAAAGGAAAAATATGATCCTCCAGATCCTCGCAGAATTTATACCATCATG
  TCAGCAGAGGAGGTAGCCAATGGGAAAAAATCTCACTGGGCAGAATTAGAAATCTCGGGTAGAGTGCGG
  AGCCTAAGTACATCACTTTGGTCATTGACACACTTGACAGCGCTGCACCTAAATGACAATTACCTTAGT
  CGCATTCCACCTGATATTGCCAAGCTTCATAATCTGGTTTACCTGGATCTGTCATCCAATAAACTCAGA
  AGTTTACCAGCAGAACTAGGAAACATGGTGTCTCTCAGGGAATTGCTTTTAAATAACAATCTGTTACGG
  GTTTTGCCTTATGAACTTGGTCGGCTCTTCCAGCTACAAACTCTAGGTTTGAAAGGCAATCCTTTATCA
  CAGGATATTCTCAACTTATACCAGGACCCAGATGGAACCCGAAAGCTACTGAACTTCATGCTTGACAAT
  CTCGCAGTTCATCCAGAGCAGCTTCCTCCGAGGCCATGGATTACATTAAAAGAACGAGACCAAATTCTG
  CCGTCAGCATCATTCACGGTTATGTGTTACAATGTGTTATGTGATAAATACGCTACCCGGCAGCTATAT
  GGCTATTGCCCATCCTGGGCATTAAACTGGGAATACAGGAAAAAGGGAATTATGGAAGAAATTGTTAAC
  TGTGACGCAGATATCATTAGTCTTCAGGAAGTGGAAACAGAGCAATACTTCACTCTCTTTCTGCCAGCA
  TTGAAGGAGCGTGGATATGATGGATTTTTTTCTCCAAAGTCACGTGCCAAAATCATGTCTGAGCAGGAG
  AGAAAGCATGTAGATGGTTGTGCAATATTCTTCAAAACAGAAAAATTTACATTGGTGCAGAAGCATACA
  GTGGAATTTAACCAAGTGGCGATGGCTAATTCAGATGGATCCGAAGCTATGCTGAACAGAGTGATGACA
  AAAGATAACATTGGTGTCGCTGTGGTATTAGAGGTCCACAAAGAACTATTTGGAGCAGGTATGAAGCCT
  ATTCATGCTGCAGACAAACAGCTGCTTATAGTGGCAAATGCCCACATGCATTGGGACCCAGAGTATTCT
  GATGTGAAGCTCATCCAGACCATGATGTTTGTCTCAGAGGTTAAAAACATTCTGGAGAAAGCCTCTAGT
  AGGCCTGGAAGCCCAACTGCAGATCCTAATTCCATCCCGCTGGTGCTATGTGCAGATCTTAACTCATTG
  CCAGATTCAGGTGTTGTGGAATACTTAAGCAATGGAGGAGTAGCTGACAACCATAAAGACTTCAAGGAA
  CTAAGGTACAATGAGTGTCTTATGAACTTCAGCTGCAATGGAAAGAATGGAAGCTCAGAAGGGAGAATC
  ACACATGGCTTCCAACTTAAGAGCGCCTATGAAAATAACTTGATGCCTTACACCAATTACACCTTTGAT
  TTCAAAGGCGTGATTGACTACATTTTCTATTCCAAGACTCATATGAACGTGCTTGGTGTCCTGGGGCCT
  TTAGATCCTCAATGGCTGGTTGAGAACAACATCACTGGGTGTCCACACCCTCACATCCCTTCAGACCAC
  TTCTCACTGTTAACACAACTTGAACTCCACCCTCCACTCCTGCCTCTTGTCAATGGTGTTCACTTGCCT
  AATCGGAGGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_144571
• Insert Size: 1668 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144571.2
• RefSeq Size: 8794 bp
• RefSeq ORF: 1668 bp
• Locus ID: 246175
• UniProt ID: Q96LI5
• Cytogenetics: 4q21.1
• Domains: LRR, LRR_TYP, Exo_endo_phos, LRR_PS
• Protein Pathways: RNA degradation
• MW: 63 kDa
• Gene Summary: Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in the deadenylation-dependent degradation of mRNAs through the 3' UTR AU-rich element-mediated mechanism. Involved in deadenylation-dependent degradation of CDKN1B mRNA. Its mRNA deadenylase activity can be inhibited by TOB1. Mediates cell proliferation and cell survival and prevents cellular senescence.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.