Synaptopodin (SYNPO) (NM_001109974) Human Untagged Clone

CAT#: SC317128

SYNPO (untagged)-Human synaptopodin (SYNPO), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Synaptopodin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317128 representing NM_001109974.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGGGGTACTCAGAGGAGGCTAGCTTGCTGCGGCACCTGGAGAAGGTGGCCAGTGAGGAGGAAGAG
  GTACCACTGGTGGTTTATCTAAAGGAGAATGCAGCACTGCTGACAGCCAATGGGCTGCACCTGTCCCAA
  AACCGAGAGGCCCAGCAGTCCTCACCGGCCCCACCTCCAGCTGAGGTCCACAGCCCAGCTGCAGATGTC
  AACCAAAACCTTGCCTCGCCCAGTGCCACGCTCACCACACCAACTTCTAACAGCAGCCACAATCCGCCA
  GCCACCGATGTCAATCAGAACCCACCGGCAACTGTTGTCCCACAGAGCCTGCCACTTTCTAGCATCCAA
  CAGAATTCCTCAGAGGCCCAACTCCCATCTAATGGCACAGGGCCTGCTTCCAAACCCAGCACCCTGTGT
  GCTGATGGGCAACCCCAGGCACCGGCTGAGGAGGTGAGATGCAGCACACTCCTAATTGACAAGGTATCA
  ACTCCAGCTACCACCACCAGCACCTTCTCCAGAGAAGCTACGCTCATCCCCAGCTCCAGGCCCCCAGCC
  TCAGATTTCATGTCCAGCTCCCTGCTCATTGACATCCAGCCCAACACCCTAGTGGTGTCAGCAGATCAA
  GAGATGTCTGGGCGAGCAGCTGCCACCACGCCCACCAAGGTCTACAGTGAGGTCCACTTCACACTGGCC
  AAGCCCCCATCAGTGGTCAACAGGACGGCCAGGCCTTTTGGGATCCAGGCGCCAGGGGGCACCAGCCAG
  ATGGAGAGGAGCCCCATGCTAGAGAGACGACATTTTGGGGAGAAGGCCCCGGCTCCCCAGCCCCCCAGT
  TTGCCAGACAGGAGCCCCCGGCCACAGAGACACATAATGTCCCGCAGCCCCATGGTGGAAAGGAGGATG
  ATGGGGCAGCGAAGCCCGGCCTCAGAGAGACGCCCCTTGGGGAACTTCACTGCACCCCCCACCTACACT
  GAGACCTTGTCCACAGCCCCTCTGGCTTCCTGGGTGAGGTCTCCTCCCTCATATTCTGTCCTGTATCCC
  AGCTCCGACCCCAAGTCTTCTCATCTGAAGGGCCAGGCGGTTCCTGCCAGCAAGACGGGCATTCTGGAG
  GAGTCGATGGCCCGCCGGGGCAGCCGCAAATCCATGTTTACTTTCGTGGAGAAGCCCAAGGTGACCCCG
  AATCCAGACTTGCTGGATCTGGTACAGACAGCGGATGAGAAGCGGCGGCAGAGGGACCAGGGGGAGGTA
  GGCGTGGAGGAGGAGCCCTTCGCACTGGGGGCCGAGGCCTCCAACTTCCAGCAGGAGCCAGCACCTCGT
  GACAGGGCCAGCCCCGCGGCGGCGGAGGAGGTGGTACCAGAGTGGGCCTCCTGCCTCAAGTCACCCCGC
  ATCCAGGCCAAGCCGAAGCCCAAACCCAACCAGAACCTCTCCGAGGCCTCTGGGAAGGGAGCTGAGCTC
  TACGCCCGCCGCCAGTCACGGATGGAGAAATATGTCATCGAGTCTTCAAGCCACACGCCAGAGCTGGCC
  CGCTGCCCATCACCTACCATGTCCCTGCCTTCCTCCTGGAAATACCCCACTAACGCCCCCGGGGCCTTC
  CGAGTGGCATCCCGAAGCCCAGCCCGGACCCCGCCTGCCTCCCTCTACCATGGCTACCTGCCTGAGAAC
  GGGGTCCTGCGCCCAGAGCCCACCAAGCAGCCGCCATACCAGCTGCGCCCCTCGCTCTTTGTCCTCTCA
  CCTATCAAGGAGCCTGCCAAGGTCTCACCAAGAGCTGCCTCGCCCGCCAAGCCCAGCTCCTTGGACCTG
  GTGCCCAACCTGCCCAAGGGGGCTCTCCCTCCATCTCCTGCCCTGCCTCGGCCCTCGCGCTCCTCACCG
  GGCCTCTACACCTCCCCCGGCCAGGACAGCCTGCAGCCCACTGCCGTGAGCCCTCCTTACGGCGGTGAC
  ATCTCCCCCGTGTCTCCCTCCAGGGCGTGGTCTCCCCGAGCCAAGCAGGCCCCCAGGCCCTCCTTCTCT
  ACCCGGAACGCCGGGATCGAGGCTCAGGTGTGGAAGCCTTCCTTCTGCTTCAAGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001109974
• Insert Size: 2058 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109974.2
• RefSeq Size: 4617 bp
• RefSeq ORF: 2058 bp
• Locus ID: 11346
• UniProt ID: Q8N3V7
• Cytogenetics: 5q33.1
• MW: 73.7 kDa
• Gene Summary: Synaptopodin is an actin-associated protein that may play a role in actin-based cell shape and motility. The name synaptopodin derives from the protein's associations with postsynaptic densities and dendritic spines and with renal podocytes (Mundel et al., 1997 [PubMed 9314539]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (B), also referred to as 'Synpo-short', has a distinct C-terminus and is shorter than isoform 1.