USP5 (NM_001098536) Human Untagged Clone

CAT#: SC316428

USP5 (untagged)-Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP5
• Synonyms: ISOT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC316428 representing NM_001098536.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGAGCTGAGTGAGGAGGCGCTGCTGTCAGTATTACCGACGATCCGGGTCCCTAAGGCTGGAGAC
  CGGGTCCACAAAGACGAGTGCGCCTTCTCCTTCGACACGCCGGAGTCTGAGGGGGGCCTCTACATCTGT
  ATGAACACGTTTCTGGGCTTTGGGAAACAGTATGTGGAGAGACATTTCAATAAGACCGGCCAGCGAGTC
  TACTTGCACCTCCGGCGGACCCGGCGCCCGAAAGAGGAGGACCCTGCTACAGGCACTGGAGACCCACCC
  CGGAAGAAGCCCACGCGGCTGGCTATTGGTGTTGAAGGCGGATTTGACCTTAGCGAGGAGAAGTTTGAA
  TTAGACGAGGATGTGAAGATTGTCATTTTGCCAGATTACCTGGAGATTGCCCGGGATGGACTGGGGGGA
  CTGCCTGACATTGTCAGAGATCGGGTGACCAGTGCAGTGGAGGCCCTACTGTCGGCCGACTCAGCCTCC
  CGCAAGCAGGAGGTGCAGGCATGGGATGGGGAAGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAG
  CAGTTGGACAACCCTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGAGAGAAC
  CTGTGGCTCAACCTGACTGATGGCTCCATCCTCTGTGGGCGACGCTACTTCGATGGCAGTGGGGGCAAC
  AACCACGCTGTGGAGCACTACCGAGAGACAGGCTACCCGTTAGCTGTCAAGCTGGGCACCATCACCCCT
  GATGGAGCTGACGTGTACTCATATGATGAGGATGACATGGTCCTGGACCCCAGCCTGGCTGAGCACCTG
  TCCCACTTCGGCATCGACATGCTGAAGATGCAGAAGACAGACAAGACGATGACTGAGTTGGAGATAGAC
  ATGAACCAGCGGATTGGTGAATGGGAGCTGATCCAGGAGTCAGGTGTGCCACTCAAGCCCCTGTTTGGG
  CCTGGCTACACAGGCATCCGGAACCTGGGTAACAGCTGCTACCTCAACTCTGTGGTCCAGGTGCTCTTC
  AGCATCCCTGACTTCCAGAGGAAGTATGTGGATAAGCTGGAGAAGATCTTCCAGAATGCCCCGACGGAC
  CCTACCCAGGATTTCAGCACCCAGGTGGCCAAGCTGGGCCATGGCCTTCTCTCCGGGGAGTATTCCAAG
  CCAGTACCGGAGTCGGGCGATGGGGAGCGGGTGCCAGAACAGAAGGAAGTTCAAGATGGCATTGCCCCT
  CGGATGTTCAAGGCCCTCATCGGCAAGGGCCACCCTGAATTCTCCACCAACCGGCAGCAGGATGCCCAG
  GAGTTCTTCCTTCACCTTATCAACATGGTGGAGAGGAATTGCCGGAGCTCTGAAAATCCTAATGAAGTG
  TTCCGCTTCTTGGTGGAGGAAAAGATCAAGTGCCTGGCCACAGAGAAGGTGAAGTACACCCAGCGAGTT
  GACTACATCATGCAGCTGCCTGTGCCCATGGATGCAGCCCTTAACAAAGAGGAGCTTCTGGAGTACGAG
  GAGAAGAAGCGGCAAGCCGAAGAGGAGAAGATGGCACTGCCAGAACTGGTTCGGGCCCAGGTGCCCTTC
  AGCTCTTGCCTGGAGGCCTACGGGGCCCCTGAGCAGGTCGATGACTTCTGGAGCACGGCCCTGCAGGCC
  AAGTCAGTAGCTGTCAAGACCACACGATTTGCCTCATTCCCTGACTACCTGGTCATCCAGATCAAGAAG
  TTCACCTTCGGCTTAGACTGGGTGCCCAAGAAACTGGATGTGTCCATCGAGATGCCAGAGGAGCTCGAC
  ATCTCCCAGTTGAGGGGCACAGGGCTGCAGCCCGGAGAGGAGGAGCTGCCAGACATTGCCCCACCCCTG
  GTCACTCCGGATGAGCCCAAAGGTAGCCTTGGTTTCTATGGCAACGAAGACGAAGACTCCTTCTGCTCC
  CCTCACTTCTCCTCTCCGACATCGCCCATGCTGGATGAATCAGTCATCATCCAGCTGGTGGAGATGGGA
  TTCCCTATGGACGCCTGCCGCAAAGCTGTCTACTACACGGGCAACAGCGGGGCTGAGGCCGCCATGAAC
  TGGGTCATGTCACACATGGATGATCCAGATTTTGCAAACCCCCTCATCCTGCCTGGCTCTAGTGGGCCG
  GGCTCCACAAGCGCAGCAGCCGACCCCCCTCCTGAGGACTGTGTGACCACCATTGTCTCCATGGGCTTC
  TCCCGGGACCAGGCCTTGAAAGCGCTGCGGGCCACGAACAATAGTTTAGAACGGGCTGTGGACTGGATC
  TTCAGTCACATTGACGACCTGGATGCTGAAGCTGCCATGGACATCTCAGAGGGCCGCTCAGCTGCCGAC
  TCCATCTCTGAGTCTGTGCCAGTGGGACCTAAAGTCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCC
  TTCATTAGTCACATGGGCACCTCTACCATGTGTGGTCACTACGTCTGCCACATCAAGAAAGAAGGCAGA
  TGGGTGATCTACAATGACCAGAAAGTGTGTGCCTCCGAGAAGCCGCCCAAGGACCTGGGCTACATCTAC
  TTCTACCAGAGAGTGGCCAGCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001098536
• Insert Size: 2577 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098536.1
• RefSeq Size: 3214 bp
• RefSeq ORF: 2577 bp
• Locus ID: 8078
• UniProt ID: P45974
• Cytogenetics: 12p13.31
• Protein Families: Druggable Genome, Protease
• MW: 95.8 kDa
• Gene Summary: Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010]
  Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1).