USP5 (NM_001098536) Human Tagged ORF Clone

CAT#: RG224191

USP5 (tGFP-tagged) - Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1

Product Images

Circular map for RG224191

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: USP5
• Synonyms: ISOT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224191 representing NM_001098536
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGAGTGAGGAGGCGCTGCTGTCAGTATTACCGACGATCCGGGTCCCTAAGGCTGGAGACC
  GGGTCCACAAAGACGAGTGCGCCTTCTCCTTCGACACGCCGGAGTCTGAGGGGGGCCTCTACATCTGTAT
  GAACACGTTTCTGGGCTTTGGGAAACAGTATGTGGAGAGACATTTCAATAAGACCGGCCAGCGAGTCTAC
  TTGCACCTCCGGCGGACCCGGCGCCCGAAAGAGGAGGACCCTGCTACAGGCACTGGAGACCCACCCCGGA
  AGAAGCCCACGCGGCTGGCTATTGGTGTTGAAGGCGGATTTGACCTTAGCGAGGAGAAGTTTGAATTAGA
  CGAGGATGTGAAGATTGTCATTTTGCCAGATTACCTGGAGATTGCCCGGGATGGACTGGGGGGACTGCCT
  GACATTGTCAGAGATCGGGTGACCAGTGCAGTGGAGGCCCTACTGTCGGCCGACTCAGCCTCCCGCAAGC
  AGGAGGTGCAGGCATGGGATGGGGAAGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAGCAGTTGGA
  CAACCCTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGAGAGAACCTGTGGCTC
  AACCTGACTGATGGCTCCATCCTCTGTGGGCGACGCTACTTCGATGGCAGTGGGGGCAACAACCACGCTG
  TGGAGCACTACCGAGAGACAGGCTACCCGTTAGCTGTCAAGCTGGGCACCATCACCCCTGATGGAGCTGA
  CGTGTACTCATATGATGAGGATGACATGGTCCTGGACCCCAGCCTGGCTGAGCACCTGTCCCACTTCGGC
  ATCGACATGCTGAAGATGCAGAAGACAGACAAGACGATGACTGAGTTGGAGATAGACATGAACCAGCGGA
  TTGGTGAATGGGAGCTGATCCAGGAGTCAGGTGTGCCACTCAAGCCCCTGTTTGGGCCTGGCTACACAGG
  CATCCGGAACCTGGGTAACAGCTGCTACCTCAACTCTGTGGTCCAGGTGCTCTTCAGCATCCCTGACTTC
  CAGAGGAAGTATGTGGATAAGCTGGAGAAGATCTTCCAGAATGCCCCGACGGACCCTACCCAGGATTTCA
  GCACCCAGGTGGCCAAGCTGGGCCATGGCCTTCTCTCCGGGGAGTATTCCAAGCCAGTACCGGAGTCGGG
  CGATGGGGAGCGGGTGCCAGAACAGAAGGAAGTTCAAGATGGCATTGCCCCTCGGATGTTCAAGGCCCTC
  ATCGGCAAGGGCCACCCTGAATTCTCCACCAACCGGCAGCAGGATGCCCAGGAGTTCTTCCTTCACCTTA
  TCAACATGGTGGAGAGGAATTGCCGGAGCTCTGAAAATCCTAATGAAGTGTTCCGCTTCTTGGTGGAGGA
  AAAGATCAAGTGCCTGGCCACAGAGAAGGTGAAGTACACCCAGCGAGTTGACTACATCATGCAGCTGCCT
  GTGCCCATGGATGCAGCCCTTAACAAAGAGGAGCTTCTGGAGTACGAGGAGAAGAAGCGGCAAGCCGAAG
  AGGAGAAGATGGCACTGCCAGAACTGGTTCGGGCCCAGGTGCCCTTCAGCTCTTGCCTGGAGGCCTACGG
  GGCCCCTGAGCAGGTCGATGACTTCTGGAGCACGGCCCTGCAGGCCAAGTCAGTAGCTGTCAAGACCACA
  CGATTTGCCTCATTCCCTGACTACCTGGTCATCCAGATCAAGAAGTTCACCTTCGGCTTAGACTGGGTGC
  CCAAGAAACTGGATGTGTCCATCGAGATGCCAGAGGAGCTCGACATCTCCCAGTTGAGGGGCACAGGGCT
  GCAGCCCGGAGAGGAGGAGCTGCCAGACATTGCCCCACCCCTGGTCACTCCGGATGAGCCCAAAGGTAGC
  CTTGGTTTCTATGGCAACGAAGACGAAGACTCCTTCTGCTCCCCTCACTTCTCCTCTCCGACATCGCCCA
  TGCTGGATGAATCAGTCATCATCCAGCTGGTGGAGATGGGATTCCCTATGGACGCCTGCCGCAAAGCTGT
  CTACTACACGGGCAACAGCGGGGCTGAGGCCGCCATGAACTGGGTCATGTCACACATGGATGATCCAGAT
  TTTGCAAACCCCCTCATCCTGCCTGGCTCTAGTGGGCCGGGCTCCACAAGCGCAGCAGCCGACCCCCCTC
  CTGAGGACTGTGTGACCACCATTGTCTCCATGGGCTTCTCCCGGGACCAGGCCTTGAAAGCGCTGCGGGC
  CACGAACAATAGTTTAGAACGGGCTGTGGACTGGATCTTCAGTCACATTGACGACCTGGATGCTGAAGCT
  GCCATGGACATCTCAGAGGGCCGCTCAGCTGCCGACTCCATCTCTGAGTCTGTGCCAGTGGGACCTAAAG
  TCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCCTTCATTAGTCACATGGGCACCTCTACCATGTGTGG
  TCACTACGTCTGCCACATCAAGAAAGAAGGCAGATGGGTGATCTACAATGACCAGAAAGTGTGTGCCTCC
  GAGAAGCCGCCCAAGGACCTGGGCTACATCTACTTCTACCAGAGAGTGGCCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001098536
• ORF Size: 2574 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098536.2
• RefSeq Size: 3214 bp
• RefSeq ORF: 2577 bp
• Locus ID: 8078
• UniProt ID: P45974
• Cytogenetics: 12p13.31
• Protein Families: Druggable Genome, Protease
• Gene Summary: Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010]