DIAPH1 (NM_001079812) Human Untagged Clone

CAT#: SC315877

DIAPH1 (untagged)-Human diaphanous homolog 1 (Drosophila) (DIAPH1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DIAPH1
• Synonyms: DFNA1; DIA1; DRF1; hDIA1; LFHL1; SCBMS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001079812 edited
  GCCAGCGTGAACCGGGACATGGAGCCGCCCGGCGGGAGCCTGGGGCCCGGCCGCGGGACC
  CGGGACAAGAAGAAGGGCCGGAGCCCAGATGAGCTGCCCTCGGCGGGCGGCGACGGCGGC
  AAATCTAAGAAATTTCTGGAGAGATTTACCAGCATGAGAATTAAGAAGGAGAAGGAAAAG
  CCCAATTCTGCTCATAGAAATTCTTCTGCATCATATGGGGATGATCCCACAGCACAGTCA
  TTGCAAGATGTTTCAGATGAACAAGTGCTGGTTCTCTTTGAACAGATGCTGCTGGATATG
  AACCTGAATGAGGAGAAACAGCAACCTTTGAGGGAGAAGGACATCATCATCAAGAGGGAG
  ATGGTGTCCCAATACTTGTACACCTCCAAGGCTGGCATGAGCCAGAAGGAGAGCTCTAAG
  TCTGCCATGATGTATATTCAGGAGTTGAGGTCAGGCTTGCGGGATATGCCTCTGCTCAGC
  TGCCTGGAGTCCCTTCGTGTGTCTCTCAACAACAACCCTGTCAGTTGGGTGCAAACATTT
  GGTGCTGAAGGCTTGGCCTCCTTATTGGACATTCTTAAACGACTTCATGATGAGAAAGAA
  GAGACTGCTGGGAGTTACGATAGCCGGAACAAGCATGAGATCATTCGCTGCTTGAAAGCT
  TTTATGAACAACAAGTTTGGAATCAAGACCATGTTGGAGACAGAAGAAGGAATCCTACTG
  CTGGTCAGAGCCATGGATCCTGCTGTTCCCAACATGATGATTGATGCAGCTAAGCTGCTT
  TCTGCTCTTTGTATTCTACCGCAGCCAGAGGACATGAATGAAAGGGTTTTGGAGGCAATG
  ACAGAAAGAGCTGAGATGGATGAAGTGGAACGTTTCCAGCCGCTGCTGGATGGATTAAAA
  AGTGGAACCACTATTGCACTGAAGGTTGGATGCCTACAGCTGATCAATGCTCTCATCACA
  CCAGCGGAGGAACTTGACTTCCGAGTTCACATCAGAAGTGAACTGATGCGTTTGGGGCTA
  CATCAGGTGTTGCAGGACCTTCGAGAGATTGAAAATGAAGATATGAGAGTGCAACTAAAT
  GTGTTTGATGAACAAGGGGAAGAGGATTCCTATGACCTGAAGGGACGGCTGGATGACATT
  CGCATGGAGATGGATGACTTTAATGAAGTCTTTCAGATTCTCTTAAACACAGTGAAGGAT
  TCAAAGGCAGAGCCACACTTCCTTTCCATCCTGCAGCACTTACTCTTGGTCCGAAATGAC
  TATGAGGCCAGACCTCAGTACTATAAGTTGATTGAAGAATGTATTTCCCAGATAGTTCTG
  CACAAGAACGGGGCTGATCCTGACTTCAAGTGCCGGCACCTCCAGATTGAGATTGAGGGA
  TTAATTGATCAAATGATTGATAAGACAAAGGTGGAGAAATCTGAAGCCAAAGCTGCAGAG
  CTGGAAAAGAAGTTGGACTCAGAGTTAACAGCCCGACATGAGCTACAGGTGGAAATGAAA
  AAGATGGAAAGTGACTTTGAGCAGAAGCTTCAAGATCTTCAGGGAGAAAAAGATGCACTG
  CATTCTGAAAAGCAGCAAATTGCCACAGAGAAACAGGACCTGGAAGCAGAGGTGTCCCAG
  CTCACAGGAGAGGTTGCCAAGCTGACAAAGGAACTGGAAGATGCCAAGAAAGAAATGGCT
  TCCCTCTCTGCGGCAGCTATTACTGTACCTCCTTCTGTTCCTAGTCGTGCTCCTGTTCCC
  CCTGCCCCTCCTTTACCTGGTGACTCTGGCACTATTATTCCACCACCACCTGCTCCTGGG
  GATAGTACCACTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCACCTCCTTTGCCTGGGGGT
  GTTTGCATCTCCTCACCCCCTTCTTTACCTGGAGGTACTGCTATCTCTCCACCCCCTCCT
  TTGTCTGGGGATGCTACCATCCCTCCACCCCCTCCTTTGCCTGAGGGTGTTGGCATCCCT
  TCACCCTCTTCTTTGCCTGGAGGTACTGCCATCCCCCCACCTCCTCCTTTGCCTGGGAGT
  GCTAGAATCCCCCCACCACCACCTCCTTTGCCTGGGAGTGCTGGAATTCCCCCCCCACCT
  CCTCCCTTGCCTGGAGAAGCAGGAATGCCACCTCCTCCTCCCCCTCTTCCTGGTGGTCCT
  GGAATCCCTCCACCTCCTCCATTTCCCGGAGGCCCTGGCATTCCTCCACCTCCACCCGGA
  ATGGGTATGCCTCCACCTCCCCCATTTGGATTTGGAGTTCCTGCAGCCCCAGTTCTGCCA
  TTTGGATTAACCCCCAAAAAGCTTTATAAGCCAGAGGTGCAGCTCCGGAGGCCAAACTGG
  TCCAAGCTTGTGGCTGAGGACCTCTCCCAGGACTGCTTCTGGACAAAGGTGAAGGAGGAC
  CGCTTTGAGAACAATGAACTTTTCGCCAAACTTACCCTTACCTTCTCTGCCCAGACCAAG
  ACTTCCAAAGCCAAGAAGGATCAAGAAGGTGGAGAAGAAAAGAAATCTGTGCAAAAGAAA
  AAAGTAAAAGAGTTAAAGGTGTTGGATTCAAAGACAGCCCAGAATCTCTCAATCTTTTTG
  GGTTCCTTCCGCATGCCCTATCAAGAGATTAAGAATGTCATCCTGGAGGTGAATGAGGCT
  GTTCTGACTGAGTCTATGATCCAGAACCTCATTAAGCAAATGCCAGAGCCAGAGCAGTTA
  AAAATGCTTTCTGAACTGAAGGATGAATATGATGACCTGGCTGAGTCAGAGCAGTTTGGC
  GTGGTGATGGGCACTGTGCCCCGACTGCGGCCTCGCCTCAATGCCATTCTCTTCAAGCTA
  CAATTCAGCGAGCAAGTGGAGAATATCAAGCCAGAGATTGTGTCTGTCACTGCTGCATGT
  GAGGAGTTACGTAAGAGTGAGAGCTTTTCCAATCTCCTAGAGATTACCTTGCTTGTTGGA
  AATTACATGAATGCTGGCTCCAGAAATGCTGGTGCTTTTGGCTTCAATATCAGCTTCCTC
  TGTAAGCTTCGAGACACCAAGTCCACAGATCAGAAGATGACGTTGTTACACTTCTTGGCT
  GAGTTGTGTGAGAATGACTATCCCGATGTCCTCAAGTTTCCAGACGAGCTTGCCCATGTG
  GAGAAAGCCAGCCGAGTTTCTGCTGAAAACTTGCAAAAGAACCTAGATCAGATGAAGAAA
  CAAATTTCTGATGTGGAACGTGATGTTCAGAATTTCCCAGCTGCCACAGATGAAAAAGAC
  AAGTTTGTTGAAAAAATGACCAGCTTTGTGAAGGATGCACAGGAACAGTATAACAAGCTG
  CGGATGATGCATTCTAACATGGAGACCCTCTATAAGGAGCTGGGCGAGTACTTCCTCTTT
  GACCCCAAGAAGTTGTCTGTTGAAGAATTTTTCATGGATCTTCACAATTTTCGGAATATG
  TTTTTGCAAGCAGTCAAGGAGAACCAGAAGCGGCGGGAGACAGAAGAAAAGATGAGGCGA
  GCAAAACTAGCCAAGGAGAAGGCAGAGAAGGAGCGGCTAGAGAAGCAGCAGAAGAGAGAG
  CAACTCATAGACATGAATGCAGAGGGCGATGAGACAGGTGTGATGGACAGTCTTCTAGAA
  GCCCTGCAGTCAGGGGCAGCATTCCGACGGAAGAGAGGGCCCCGTCAAGCCAACAGGAAG
  GCCGGGTGTGCAGTCACATCTCTGCTAGCTTCGGAGCTGACCAAGGATGATGCCATGGCT
  GCTGTTCCTGCCAAGGTGTCCAAGAACAGTGAGACATTCCCCACAATCCTTGAGGAAGCC
  AAGGAGTTGGTTGGCCGTGCAAGCTAATGTGGGTCCTGTGACCGCGGCAGCTCCTCAGCG
  GAGCCGCAGACTGTCCTGCA
  
• Restriction Sites: Please inquire
• ACCN: NM_001079812
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone is found to be a good match to NM_001079812.1 except for 3bp deletion.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079812.1, NP_001073280.1
• RefSeq Size: 5718 bp
• RefSeq ORF: 3792 bp
• Locus ID: 1729
• UniProt ID: O60610
• Cytogenetics: 5q31.3
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Focal adhesion, Regulation of actin cytoskeleton
• Gene Summary: This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, as compared to variant 1, which results in a shorter isoform (2).