CYLD (NM_001042412) Human Untagged Clone

CAT#: SC311321

CYLD (untagged)-Human cylindromatosis (turban tumor syndrome) (CYLD), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYLD
• Synonyms: BRSS; CDMT; CYLD1; CYLDI; EAC; FTDALS8; MFT; MFT1; SBS; TEM; USPL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC311321 representing NM_001042412.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGTTCAGGCTTATGGAGCCAAGAAAAAGTCACTTCACCCTACTGGGAAGAGCGGATTTTTTACTTG
  CTTCTTCAAGAATGCAGCGTTACAGACAAACAAACACAAAAGCTCCTTAAAGTACCGAAGGGAAGTATA
  GGACAGTATATTCAAGATCGTTCTGTGGGGCATTCAAGGATTCCTTCTGCAAAAGGCAAGAAAAATCAG
  ATTGGATTAAAAATTCTAGAGCAACCTCATGCAGTTCTCTTTGTTGATGAAAAGGATGTTGTAGAGATA
  AATGAAAAGTTCACAGAGTTACTTTTGGCAATTACCAATTGTGAGGAGAGGTTCAGCCTGTTTAAAAAC
  AGAAACAGACTAAGTAAAGGCCTCCAAATAGACGTGGGCTGTCCTGTGAAAGTACAGCTGAGATCTGGG
  GAAGAAAAATTTCCTGGAGTTGTACGCTTCAGAGGACCCCTGTTAGCAGAGAGGACAGTCTCCGGAATA
  TTCTTTGGAGTTGAATTGCTGGAAGAAGGTCGTGGTCAAGGTTTCACTGACGGGGTGTACCAAGGGAAA
  CAGCTTTTTCAGTGTGATGAAGATTGTGGCGTGTTTGTTGCATTGGACAAGCTAGAACTCATAGAAGAT
  GATGACACTGCATTGGAAAGTGATTACGCAGGTCCTGGGGACACAATGCAGGTCGAACTTCCTCCTTTG
  GAAATAAACTCCAGAGTTTCTTTGAAGGTTGGAGAAACAATAGAATCTGGAACAGTTATATTCTGTGAT
  GTTTTGCCAGGAAAAGAAAGCTTAGGATATTTTGTTGGTGTGGACATGGATAACCCTATTGGCAACTGG
  GATGGAAGATTTGATGGAGTGCAGCTTTGTAGTTTTGCGTGTGTTGAAAGTACAATTCTATTGCACATC
  AATGATATCATCCCAGAGAGTGTGACGCAGGAAAGGAGGCCTCCCAAACTTGCCTTTATGTCAAGAGGT
  GTTGGGGACAAAGGTTCATCCAGTCATAATAAACCAAAGGCTACAGGATCTACCTCAGACCCTGGAAAT
  AGAAACAGATCTGAATTATTTTATACCTTAAATGGGTCTTCTGTTGACTCACAACCACAATCCAAATCA
  AAAAATACATGGTACATTGATGAAGTTGCAGAAGACCCTGCAAAATCTCTTACAGAGATATCTACAGAC
  TTTGACCGTTCTTCACCACCACTCCAGCCTCCTCCTGTGAACTCACTGACCACCGAGAACAGATTCCAC
  TCTTTACCATTCAGTCTCACCAAGATGCCCAATACCAATGGAAGTATTGGCCACAGTCCACTTTCTCTG
  TCAGCCCAGTCTGTAATGGAAGAGCTAAACACTGCACCCGTCCAAGAGAGTCCACCCTTGGCCATGCCT
  CCTGGGAACTCACATGGTCTAGAAGTGGGCTCATTGGCTGAAGTTAAGGAGAACCCTCCTTTCTATGGG
  GTAATCCGTTGGATCGGTCAGCCACCAGGACTGAATGAAGTGCTCGCTGGACTGGAACTGGAAGATGAG
  TGTGCAGGCTGTACGGATGGAACCTTCAGAGGCACTCGGTATTTCACCTGTGCCCTGAAGAAGGCGCTG
  TTTGTGAAACTGAAGAGCTGCAGGCCTGACTCTAGGTTTGCATCATTGCAGCCGGTTTCCAATCAGATT
  GAGCGCTGTAACTCTTTAGCATTTGGAGGCTACTTAAGTGAAGTAGTAGAAGAAAATACTCCACCAAAA
  ATGGAAAAAGAAGGCTTGGAGATAATGATTGGGAAGAAGAAAGGCATCCAGGGTCATTACAATTCTTGT
  TACTTAGACTCAACCTTATTCTGCTTATTTGCTTTTAGTTCTGTTCTGGACACTGTGTTACTTAGACCC
  AAAGAAAAGAACGATGTAGAATATTATAGTGAAACCCAAGAGCTACTGAGGACAGAAATTGTTAATCCT
  CTGAGAATATATGGATATGTGTGTGCCACAAAAATTATGAAACTGAGGAAAATACTTGAAAAGGTGGAG
  GCTGCATCAGGATTTACCTCTGAAGAAAAAGATCCTGAGGAATTCTTGAATATTCTGTTTCATCATATT
  TTAAGGGTAGAACCTTTGCTAAAAATAAGATCAGCAGGTCAAAAGGTACAAGATTGTTACTTCTATCAA
  ATTTTTATGGAAAAAAATGAGAAAGTTGGCGTTCCCACAATTCAGCAGTTGTTAGAATGGTCTTTTATC
  AACAGTAACCTGAAATTTGCAGAGGCACCATCATGTCTGATTATTCAGATGCCTCGATTTGGAAAAGAC
  TTTAAACTATTTAAAAAAATTTTTCCTTCTCTGGAATTAAATATAACAGATTTACTTGAAGACACTCCC
  AGACAGTGCCGGATATGTGGAGGGCTTGCAATGTATGAGTGTAGAGAATGCTACGACGATCCGGACATC
  TCAGCTGGAAAAATCAAGCAGTTTTGTAAAACCTGCAACACTCAAGTCCACCTTCATCCGAAGAGGCTG
  AATCATAAATATAACCCAGTGTCACTTCCCAAAGACTTACCCGACTGGGACTGGAGACACGGCTGCATC
  CCTTGCCAGAATATGGAGTTATTTGCTGTTCTCTGCATAGAAACAAGCCACTATGTTGCTTTTGTGAAG
  TATGGGAAGGACGATTCTGCCTGGCTCTTCTTTGACAGCATGGCCGATCGGGATGGTGGTCAGAATGGC
  TTCAACATTCCTCAAGTCACCCCATGCCCAGAAGTAGGAGAGTACTTGAAGATGTCTCTGGAAGACCTG
  CATTCCTTGGACTCCAGGAGAATCCAAGGCTGTGCACGAAGACTGCTTTGTGATGCATATATGTGCATG
  TACCAGAGTCCAACAATGAGTTTGTACAAATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042412
• Insert Size: 2862 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042412.1
• RefSeq Size: 8608 bp
• RefSeq ORF: 2862 bp
• Locus ID: 1540
• UniProt ID: Q9NQC7
• Cytogenetics: 16q12.1
• Protein Families: Druggable Genome
• Protein Pathways: RIG-I-like receptor signaling pathway
• MW: 107 kDa
• Gene Summary: This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) has an alternate 5' terminal exon, and lacks one exon in the 5' UTR and another in-frame exon in the coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Variants 2 and 3 encode the same isoform.