REC114 (NM_001042367) Human Untagged Clone

CAT#: SC311247

C15orf60 (untagged)-Human chromosome 15 open reading frame 60 (C15orf60)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: REC114
• Synonyms: C15orf60; CT147; OOMD10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC311247 representing NM_001042367.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGAGGCAGGAAAAGTGCCCTTGAGCCTCGGGCTTACCGGAGGAGAAGCGGCAGAGTGGCCTCTG
  CAGCGGTACGCCCGCTGCATACCCTCAAACACCAGAGACCCACCTGGGCCATGCCTGGAAGCTGGGACA
  GCCCCCTGCCCCACATGGAAGGTTTTTGATTCCAATGAAGAATCTGGATATCTTGTTCTCACCATAGTT
  ATATCAGGTCATTTCTTCATTTTCCAAGGACAGACACTACTGGAAGGGTTTTCACTCATTGGTAGCAAG
  GACTGGTTGAAGATTGTAAGACGCGTGGATTGTCTGTTGTTTGGAACAACGATAAAGGACAAGAGTCGC
  CTGTTTCGAGTACAGTTCAGTGGAGAGTCAAAGGAGCAGGCGCTGGAACACTGCTGCAGTTGTGTTCAG
  AAGCTGGCACAATACATAACCGTGCAGGTGCCTGATGGAAACATCCAGGAGCTTCAGCTGATTCCTGGC
  CCACCCAGGGCAACTGAAAGTCAAGGGAAGGATTCTGCAAAGAGTGTCCCACGGCAGCCTGGATCCCAC
  CAGCACTCAGAACAACAGCAAGTGTGTGTAACAGCGGGCACAGGCGCTCCAGACGGAAGGACCTCACTG
  ACGCAGTTAGCTCAGACTCTTCTGGCATCGGAGGAGCTGCCCCATGTCTATGAACAATCTGCATGGGGT
  GCAGAAGAGTTAGGCCCCTTCCTACGTTTGTGCCTTATGGATCAGAATTTCCCAGCATTTGTGGAAGAG
  GTAGAAAAGGAACTGAAAAAGCTGGCGGGTTTGAGAAATTAA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001042367
• Insert Size: 801 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042367.1
• RefSeq Size: 938 bp
• RefSeq ORF: 801 bp
• Locus ID: 283677
• UniProt ID: Q7Z4M0
• Cytogenetics: 15q24.1
• MW: 29.2 kDa
• Gene Summary: The protein encoded by this gene is orthologous to the mouse meiotic recombination protein REC114, which is involved in DNA double-strand break formation during meiosis. The encoded protein is conserved in most eukaryotes and was first discovered and characterized in yeast. [provided by RefSeq, Feb 2017]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).