PIGB (NM_004855) Human Untagged Clone

CAT#: SC310271

PIGB (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class B (PIGB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIGB
• Synonyms: DEE80; EIEE80; GPI-MT-III; PIG-B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310271 representing NM_004855.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGGAGGCCCCTAAGCAAGTGCGGAATGGAGCCGGGGGGCGGAGATGCCAGCCTCACTTTGCATGGT
  CTCCAGAACCGCTCCCACGGCAAGATAAAGCTGCGAAAGAGAAAGTCTACCTTGTACTTCAACACCCAG
  GAGAAGAGCGCCAGGCGCCGCGGGGATCTTCTTGGAGAAAATATTTATCTGCTCTTGTTTACCATAGCT
  TTACGAATATTAAACTGCTTTTTAGTGCAGACAAGTTTTGTTCCAGATGAATACTGGCAGTCTCTTGAA
  GTTTCACATCACATGGTTTTCAATTATGGTTATTTGACTTGGGAATGGACAGAGAGACTGAGGAGTTAC
  ACTTATCCCTTAATCTTTGCAAGCATTTACAAGATTCTTCATCTTTTAGGGAAAGATAGTGTTCAGTTG
  CTGATTTGGATTCCTAGACTTGCCCAAGCACTTCTGTCTGCTGTAGCAGATGTGAGACTTTACTCATTA
  ATGAAGCAACTAGAAAATCAGGAAGTGGCAAGATGGGTGTTTTTTTGCCAGTTGTGCTCCTGGTTCACA
  TGGTATTGCTGTACCAGAACCCTTACAAACACCATGGAAACTGTTCTCACTATAATTGCTCTTTTCTAC
  TATCCTTTGGAAGGTTCAAAGTCTATGAACAGTGTCAAATACTCATCCCTGGTGGCACTTGCCTTCATA
  ATTCGTCCCACAGCTGTCATTCTGTGGACACCTTTGCTCTTCAGACATTTCTGTCAAGAACCAAGAAAG
  CTTGATCTTATTCTACATCATTTTTTACCTGTAGGCTTTGTTACTTTGAGTTTGTCTCTGATGATTGAT
  CGTATTTTTTTTGGCCAATGGACTCTGGTTCAATTTAATTTTTTGAAATTTAACGTGCTGCAGAACTGG
  GGAACATTTTATGGTTCTCATCCATGGCACTGGTACTTCAGTCAAGGATTTCCAGTTATCTTGGGTACT
  CACTTACCCTTCTTTATTCATGGCTGCTATCTAGCACCAAAGAGATACCGGATACTTTTGGTGACTGTG
  CTGTGGACACTGCTTGTTTATAGCATGTTGAGCCACAAAGAATTCAGGTTTATTTATCCAGTTTTACCA
  TTCTGTATGGTGTTCTGTGGATACTCATTAACCCACCTGAAAACATGGAAGAAACCAGCTCTAAGTTTC
  CTGTTTTTATCAAATTTGTTCCTCGCCCTTTATACTGGTTTAGTTCATCAACGAGGTACTCTTGATGTC
  ATGAGTCATATTCAAAAAGTTTGTTACAACAATCCCAATAAATCTTCAGCTTCAATATTTATAATGATG
  CCTTGCCACTCTACTCCTTATTACAGCCATGTTCACTGCCCACTTCCCATGAGATTTCTCCAGTGCCCG
  CCAGACCTGACTGGAAAAAGTCATTATCTTGATGAAGCAGATGTATTTTACCTAAATCCCTTAAACTGG
  TTACATAGAGAGTTTCATGATGATGCATCATTGCCTACTCACTTGATCACCTTCAGCATTTTGGAAGAG
  GAAATAAGCGCTTTCCTAATTTCAAGCAATTATAAAAGAACTGCTGTTTTCTTCCACACTCACTTGCCA
  GAGGGTCGAATTGGAAGTCACATATATGTCTATGAACGGAAGTTAAAAGGGAAATTCAACATGAAGATG
  AAATTCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004855
• Insert Size: 1665 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004855.4
• RefSeq Size: 2227 bp
• RefSeq ORF: 1665 bp
• Locus ID: 9488
• UniProt ID: Q92521
• Cytogenetics: 15q21.3
• Domains: PMP
• Protein Families: Transmembrane
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• MW: 65.1 kDa
• Gene Summary: This gene encodes a transmembrane protein that is located in the endoplasmic reticulum and is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene is thought to encode a member of a family of dolichol-phosphate-mannose (Dol-P-Man) dependent mannosyltransferases. [provided by RefSeq, Jul 2008]