PIGB (NM_004855) Human Tagged ORF Clone

CAT#: RG204441

PIGB (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class B (PIGB)

Product Images

Circular map for RG204441

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PIGB
• Synonyms: DEE80; EIEE80; GPI-MT-III; PIG-B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204441 representing NM_004855
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGGCCCCTAAGCAAGTGCGGAATGGAGCCGGGGGGCGGAGATGCCAGCCTCACTTTGCATGGTC
  TCCAGAACCGCTCCCACGGCAAGATAAAGCTGCGAAAGAGAAAGTCTACCTTGTACTTCAACACCCAGGA
  GAAGAGCGCCAGGCGCCGCGGGGATCTTCTTGGAGAAAATATTTATCTGCTCTTGTTTACCTTAGCTTTA
  CGAATATTAAACTGCTTTTTAGTGCAGACAAGTTTTGTTCCAGATGAATACTGGCAGTCTCTTGAAGTTT
  CACATCACATGGTTTTCAATTATGGTTATTTGACTTGGGAATGGACAGAGAGACTGAGGAGTTACACTTA
  TCCCTTAATCTTTGCAAGCATTTACAAGATTCTTCATCTTTTAGGGAAAGATAGTGTTCAGTTGCTGATT
  TGGATTCCTAGACTTGCCCAAGCACTTCTGTCTGCTGTAGCAGATGTGAGACTTTACTCATTAATGAAGC
  AACTAGAAAATCAGGAAGTGGCAAGATGGGTGTTTTTTTGCCAGTTGTGCTCCTGGTTCACATGGTATTG
  CTGTACCAGAACCCTTACAAACACCATGGAAACTGTTCTCACTATAATTGCTCTTTTCTACTATCCTTTG
  GAAGGTTCAAAGTCTATGAACAGTGTCAAATACTCATCCCTGGTGGCACTTGCCTTCATAATTCGTCCCA
  CAGCTGTCATTCTGTGGACACCTTTGCTCTTCAGACATTTCTGTCAAGAACCAAGAAAGCTTGATCTTAT
  TCTACATCATTTTTTACCTGTAGGCTTTGTTACTTTGAGTTTGTCTCTGATGATTGATCGTATTTTTTTT
  GGCCAATGGACTCTGGTTCAATTTAATTTTTTGAAATTTAACGTGCTGCAGAACTTGGGAACATTTTATG
  GTTCTCATCCATGGCACTGGTACTTCAGTCAAGGATTTCCAGTTATCTTGGGTACTCACTTACCCTTCTT
  TATTCATGGCTGCTATCTAGCACCAAAGAGATACCGGATACTTTTGGTGACTGTGCTGTGGACACTGCTT
  GTTTATAGCATGTTGAGCCACAAAGAATTCAGGTTTATTTATCCAGTTTTACCATTCTGTATGGTGTTCT
  GTGGATACTCATTAACCCACCTGAAAACATGGAAGAAACCAGCTCTAAGTTTCCTGTTTTTATCAAATTT
  GTTCCTCGCCCTTTATACTGGTTTAGTTCATCAACGAGGTACTCTTGATGTCATGAGTCATATTCAAAAA
  GTTTGTTACAACAATCCCAATAAATCTTCAGCTTCAATATTTATAATGATGCCTTGCCACTCTACTCCTT
  ATTACAGCCATGTTCACTGCCCACTTCCCATGAGATTTCTCCAGTGCCCGCCAGACCTGACTGGAAAAAG
  TCATTATCTTGATGAAGCAGATGTATTTTACCTAAATCCCTTAAACTGGTCACATAGAGAGTTTCATGAT
  GATGCATCATTGCCTACTCACTTGATCACCTTCAGCATTTTGGAAGAGGAAATAAGTGCTTTCCTAATTT
  CAAGCAATTATAAAAGAACTGCTGTTTTCTTCCACACTCACTTGCCAGAGGGTCGAATTGGAAGTCACAT
  ATATGTCTATGAACGGAAGTTAAAAGGGAAATTCAACATGAAGATGAAATTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204441 representing NM_004855
  Red=Cloning site Green=Tags(s)
  
  MRRPLSKCGMEPGGGDASLTLHGLQNRSHGKIKLRKRKSTLYFNTQEKSARRRGDLLGENIYLLLFTLAL
  RILNCFLVQTSFVPDEYWQSLEVSHHMVFNYGYLTWEWTERLRSYTYPLIFASIYKILHLLGKDSVQLLI
  WIPRLAQALLSAVADVRLYSLMKQLENQEVARWVFFCQLCSWFTWYCCTRTLTNTMETVLTIIALFYYPL
  EGSKSMNSVKYSSLVALAFIIRPTAVILWTPLLFRHFCQEPRKLDLILHHFLPVGFVTLSLSLMIDRIFF
  GQWTLVQFNFLKFNVLQNLGTFYGSHPWHWYFSQGFPVILGTHLPFFIHGCYLAPKRYRILLVTVLWTLL
  VYSMLSHKEFRFIYPVLPFCMVFCGYSLTHLKTWKKPALSFLFLSNLFLALYTGLVHQRGTLDVMSHIQK
  VCYNNPNKSSASIFIMMPCHSTPYYSHVHCPLPMRFLQCPPDLTGKSHYLDEADVFYLNPLNWSHREFHD
  DASLPTHLITFSILEEEISAFLISSNYKRTAVFFHTHLPEGRIGSHIYVYERKLKGKFNMKMKF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004855
• ORF Size: 1662 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004855.2
• RefSeq Size: 2227 bp
• RefSeq ORF: 1665 bp
• Locus ID: 9488
• UniProt ID: Q92521
• Cytogenetics: 15q21.3
• Domains: PMP
• Protein Families: Transmembrane
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• Gene Summary: This gene encodes a transmembrane protein that is located in the endoplasmic reticulum and is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene is thought to encode a member of a family of dolichol-phosphate-mannose (Dol-P-Man) dependent mannosyltransferases. [provided by RefSeq, Jul 2008]