CES2 (NM_198061) Human Untagged Clone

CAT#: SC310219

CES2 (untagged)-Human carboxylesterase 2 (CES2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CES2
• Synonyms: CE-2; CES2A1; iCE; PCE-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310219 representing NM_198061.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACTGCTCAGTCCCGCTCTCCTACCACACCCACCTTTCCCGGCCCAAGCCAGCGCACCCCGCTGACT
  CCCTGCCCAGTCCAAACTCCAAGGCTGGGCAAGGCACTGATCCACTGCTGGACAGACCCGGGGCAGCCT
  CTGGGTGAACAGCAGCGTGTCCGCCGGCAGCGAACCGAGACCAGCGAGCCGACCATGCGGCTGCACAGA
  CTTCGTGCGCGGCTGAGCGCGGTGGCCTGTGGGCTTCTGCTGCTTCTTGTCCGGGGCCAGGGCCAGGAC
  TCAGCCAGTCCCATCCGGACCACACACACGGGGCAGGTGCTGGGGAGTCTTGTCCATGTGAAGGGCGCC
  AATGCCGGGGTCCAAACCTTCCTGGGAATTCCATTTGCCAAGCCACCTCTAGGTCCGCTGCGATTTGCA
  CCCCCTGAGCCCCCTGAATCTTGGAGTGGTGTGAGGGATGGAACCACCCATCCGGCCATGTGTCTACAG
  GACCTCACCGCAGTGGAGTCAGAGTTTCTTAGCCAGTTCAACATGACCTTCCCTTCCGACTCCATGTCT
  GAGGACTGCCTGTACCTCAGCATCTACACGCCGGCCCATAGCCATGAAGGCTCTAACCTGCCGGTGATG
  GTGTGGATCCACGGTGGTGCGCTTGTTTTTGGCATGGCTTCCTTGTATGATGGTTCCATGCTGGCTGCC
  TTGGAGAACGTGGTGGTGGTCATCATCCAGTACCGCCTGGGTGTCCTGGGCTTCTTCAGCACTGGAGAC
  AAGCACGCAACCGGCAACTGGGGCTACCTGGACCAAGTGGCTGCACTACGCTGGGTCCAGCAGAATATC
  GCCCACTTTGGAGGCAACCCTGACCGTGTCACCATTTTTGGCGAGTCTGCGGGTGGCACGAGTGTGTCT
  TCGCTTGTTGTGTCCCCCATATCCCAAGGACTCTTCCACGGAGCCATCATGGAGAGTGGCGTGGCCCTC
  CTGCCCGGCCTCATTGCCAGCTCAGCTGATGTCATCTCCACGGTGGTGGCCAACCTGTCTGCCTGTGAC
  CAAGTTGACTCTGAGGCCCTGGTGGGCTGCCTGCGGGGCAAGAGTAAAGAGGAGATTCTTGCAATTAAC
  AAGCCTTTCAAGATGATCCCCGGAGTGGTGGATGGGGTCTTCCTGCCCAGGCACCCCCAGGAGCTGCTG
  GCCTCTGCCGACTTTCAGCCTGTCCCTAGCATTGTTGGTGTCAACAACAATGAATTCGGCTGGCTCATC
  CCCAAGGTCATGAGGATCTATGATACCCAGAAGGAAATGGACAGAGAGGCCTCCCAGGCTGCTCTGCAG
  AAAATGTTAACGCTGCTGATGTTGCCTCCTACATTTGGTGACCTGCTGAGGGAGGAGTACATTGGGGAC
  AATGGGGATCCCCAGACCCTCCAAGCGCAGTTCCAGGAGATGATGGCGGACTCCATGTTTGTGATCCCT
  GCACTCCAAGTAGCACATTTTCAGTGTTCCCGGGCCCCTGTGTACTTCTACGAGTTCCAGCATCAGCCC
  AGCTGGCTCAAGAACATCAGGCCACCGCACATGAAGGCAGACCATGTTAAATTCACTGAGGAAGAGGAG
  CAGCTAAGCAGGAAGATGATGAAGTACTGGGCCAACTTTGCGAGAAATGGGAACCCCAATGGCGAGGGT
  CTGCCACACTGGCCGCTGTTCGACCAGGAGGAGCAATACCTGCAGCTGAACCTACAGCCTGCGGTGGGC
  CGGGCTCTGAAGGCCCACAGGCTCCAGTTCTGGAAGAAGGCGCTGCCCCAAAAGATCCAGGAGCTCGAG
  GAGCCTGAAGAGAGACACACAGAGCTGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_198061
• Insert Size: 1824 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198061.2
• RefSeq Size: 3907 bp
• RefSeq ORF: 1824 bp
• Locus ID: 8824
• UniProt ID: O00748
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes
• MW: 67.1 kDa
• Gene Summary: This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2010]
  Transcript Variant: This variant (2) has a shorter 5' UTR and lacks an internal in-frame segment in the coding region, as compared to variant 1. The encoded isoform (2) is thus shorter than isoform 1. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by available conservation data.